Acyl-CoA-binding domain-containing protein 5 (ACBD5), partial, human, recombinant

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 1-461aa. Protein Length: Partial. Tag Info: N-terminal 6xHis-SUMO-tagged. Target Protein Sequence: MFQFHAGSWE SWCCCCLIPA DRPWDRGQHW QLEMADTRSV HETRFEAAVK VIQSLPKNGS FQPTNEMMLK FYSFYKQATE GPCKLSRPGF WDPIGRYKWD AWSSLGDMTK EEAMIAYVEE MKKIIETMPM TEKVEELLRV IGPFYEIVED KKSGRSSDIT SVRLEKISKC LEDLGNVLTS TPNAKTVNGK AESSDSGAES EEEEAQEEVK GAEQSDNDKK MMKKSADHKN LEVIVTNGYD KDGFVQDIQN DIHASSSLNG RSTEEVKPID ENLGQTGKSA VCIHQDINDD HVEDVTGIQH LTSDSDSEVY CDSMEQFGQE ESLDSFTSNN GPFQYYLGGH SSQPMENSGF REDIQVPPGN GNIGNMQVVA VEGKGEVKHG GEDGRNNSGA PHREKRGGET DEFSNVRRGR GHRMQHLSEG TKGRQVGSGG DGERWGSDRG SRGSLNEQIA LVLMRLQEDM Q. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Acyl-CoA binding protein which acts as the peroxisome receptor for pexophagy but is dispensable for aggrephagy and nonselective autophagy. Binds medium- and long-chain acyl-CoA esters. Reference: Peroxisomal Atg37 binds Atg30 or palmitoyl-CoA to regulate phagophore formation during pexophagy.Nazarko T.Y., Ozeki K., Till A., Ramakrishnan G., Lotfi P., Yan M., Subramani S.J. Cell Biol. 204:541-557(2014). Function: Acyl-CoA binding protein which acts as the peroxisome receptor for pexophagy but is dispensable for aggrephagy and nonselective autophagy. Binds medium- and long-chain acyl-CoA esters.