Acid sphingomyelinase-like phosphodiesterase 3b (Smpdl3b), mouse, recombinant

Organism: Mus musculus (Mouse). Source: E.coli. Expression Region: 19-456aa. Protein Length: Full Length of Mature Protein. Tag Info: N-terminal 10xHis-tagged and C-terminal Myc-tagged. Target Protein Sequence: QLGRFWHISD LHLDPNYTVS KDPLQVCPSA GSQPVLNAGP WGDYLCDSPW ALINSSLYAM KEIEPKPDFI LWTGDDTPHV PNESLGEAAV LAIVERLTNL IKEVFPDTKV YAALGNHDFH PKNQFPAQSN RIYNQVAELW RPWLSNESYA LFKRGAFYSE KLPGPSRAGR VVVLNTNLYY SNNEQTAGMA DPGEQFRWLG DVLSNASRDG EMVYVIGHVP PGFFEKTQNK AWFRESFNEE YLKVIQKHHR VIAGQFFGHH HTDSFRMFYD NTGAPINVMF LTPGVTPWKT TLPGVVDGAN NPGIRIFEYD RATLNLKDLV TYFLNLRQAN VQETPRWEQE YRLTEAYQVP DASVSSMHTA LTRIASEPHI LQRYYVYNSV SYNHLTCEDS CRIEHVCAIQ HVAFNTYATC LHGLGAKLVP GFLLILTLLP SLHVLEVL. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Lipid-modulating phosphodiesterase. Active on the surface of macrophages and dendritic cells and strongly influences macrophage lipid composition and membrane fluidity (PubMed:26095358). Acts as a negative regulator of Toll-like receptor signaling (PubMed:26095358, PubMed:27687724). Has in vitro phosphodiesterase activity, but the physiological substrate is unknown (PubMed:26095358, PubMed:27687724). Lacks activity with phosphocholine-containing lipids, but can cleave CDP-choline, and can release phosphate from ATP and ADP (in vitro) (PubMed:27687724). Reference: "The lipid-modifying enzyme SMPDL3B negatively regulates innate immunity." Heinz L.X., Baumann C.L., Koeberlin M.S., Snijder B., Gawish R., Shui G., Sharif O., Aspalter I.M., Mueller A.C., Kandasamy R.K., Breitwieser F.P., Pichlmair A., Bruckner M., Rebsamen M., Blueml S., Karonitsch T., Fauster A., Colinge J. Superti-Furga G. Cell Rep. 11:1919-1928(2015). Function: nan