Acetyl xylan esterase 7A, Bacillus subtilis

Acetyl xylan esterase 7A (BsAxe7A), assigned the E.C. number 3.1.1.72 and 3.1.1.41, is a derivative of Bacillus subtilis. It is an esterase that removes acetyl groups from a number of O-acetylated small substrates, such as acetylated xylose, short xylooligosaccharides and cephalosporin C. The recombinant BsAxe7A, purified from Escherichia coli, is a single-domain Carbohydrate Esterase family 7 (CE7) enzyme (see more details at www.cazy.org).The protein is supplied in a solution containing 35 mM NaHepes buffer (pH 7.5), 750 mM NaCl, 200 mM Imidazole, 3.5 mM CaCl2, and 25% (v/v) glycerol, at a concentration of 1 mg/mL. Bulk quantities of this product can be made available upon request. Shipping Conditions: Shipping: Dry Ice to RT. Storage Conditions: -30°C to -15°C. Class: Carbohydrate Esterase. Activity: Acetyl xylan esterases. Family: CE7. Ec Number: 3.1.1.72 and 3.1.1.41. Organism: Bacillus subtilis. Architecture: CE7. Optimal pH: 6.5. Optimal Temperature: 37°C. Substrates: 1,2,3,4-Tetra-O-acetyl-xylopyranose, 2,3,2',3',4'-Penta-acetyl-xylobiose, 3'-O-Acetyl-2'-deoxyadenosine, 4-nitrophenyl acetate, acetyl groups from O-acetylated small molecules, Cephalosporin C