Acetyl-coenzyme A synthetase 2-like, mitochondrial (ACSS1), human, recombinant

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 38-689aa. Protein Length: Full Length of Mature Protein. Tag Info: N-terminal 6xHis-tagged. Target Protein Sequence: ASGPSGSAPA VAAAAAQPGS YPALSAQAAR EPAAFWGPLA RDTLVWDTPY HTVWDCDFST GKIGWFLGGQ LNVSVNCLDQ HVRKSPESVA LIWERDEPGT EVRITYRELL ETTCRLANTL KRHGVHRGDR VAIYMPVSPL AVAAMLACAR IGAVHTVIFA GFSAESLAGR INDAKCKVVI TFNQGLRGGR VVELKKIVDE AVKHCPTVQH VLVAHRTDNK VHMGDLDVPL EQEMAKEDPV CAPESMGSED MLFMLYTSGS TGMPKGIVHT QAGYLLYAAL THKLVFDHQP GDIFGCVADI GWITGHSYVV YGPLCNGATS VLFESTPVYP NAGRYWETVE RLKINQFYGA PTAVRLLLKY GDAWVKKYDR SSLRTLGSVG EPINCEAWEW LHRVVGDSRC TLVDTWWQTE TGGICIAPRP SEEGAEILPA MAMRPFFGIV PVLMDEKGSV VEGSNVSGAL CISQAWPGMA RTIYGDHQRF VDAYFKAYPG YYFTGDGAYR TEGGYYQITG RMDDVINISG HRLGTAEIED AIADHPAVPE SAVIGYPHDI KGEAAFAFIV VKDSAGDSDV VVQELKSMVA TKIAKYAVPD EILVVKRLPK TRSGKVMRRL LRKIITSEAQ ELGDTTTLED PSIIAEILSV YQKCKDKQAA AK. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Important for maintaining normal body temperature during fasting and for energy homeostasis. Essential for energy expenditure under ketogenic conditions. Converts acetate to acetyl-CoA so that it can be used for oxidation through the tricarboxylic cycle to produce ATP and CO2. Reference: "Prediction of the coding sequences of unidentified human genes. XX. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro." Nagase T., Nakayama M., Nakajima D., Kikuno R., Ohara O. DNA Res. 8:85-95(2001). Function: Important for maintaining normal body temperature during fasting and for energy homeostasis. Essential for energy expenditure under ketogenic conditions (By similarity). Converts acetate to acetyl-CoA so that it can be used for oxidation through the tricarboxylic cycle to produce ATP and CO(2).