A disintegrin and metalloproteinase with thrombospondin motifs 5 (Adamts5), mouse, recombinant

Organism: Mus musculus (Mouse). Source: E.coli. Expression Region: 262-930aa. Protein Length: Full Length of Mature Protein. Tag Info: N-terminal 6xHis-tagged. Target Protein Sequence: SISRARQVEL LLVADSSMAR MYGRGLQHYL LTLASIANRL YSHASIENHI RLAVVKVVVL TDKDTSLEVS KNAATTLKNF CKWQHQHNQL GDDHEEHYDA AILFTREDLC GHHSCDTLGM ADVGTICSPE RSCAVIEDDG LHAAFTVAHE IGHLLGLSHD DSKFCEENFG TTEDKRLMSS ILTSIDASKP WSKCTSATIT EFLDDGHGNC LLDLPRKQIL GPEELPGQTY DATQQCNLTF GPEYSVCPGM DVCARLWCAV VRQGQMVCLT KKLPAVEGTP CGKGRVCLQG KCVDKTKKKY YSTSSHGNWG SWGPWGQCSR SCGGGVQFAY RHCNNPAPRN SGRYCTGKRA IYRSCSVTPC PPNGKSFRHE QCEAKNGYQS DAKGVKTFVE WVPKYAGVLP ADVCKLTCRA KGTGYYVVFS PKVTDGTECR PYSNSVCVRG RCVRTGCDGI IGSKLQYDKC GVCGGDNSSC TKIIGTFNKK SKGYTDVVRI PEGATHIKVR QFKAKDQTRF TAYLALKKKT GEYLINGKYM ISTSETIIDI NGTVMNYSGW SHRDDFLHGM GYSATKEILI VQILATDPTK ALDVRYSFFV PKKTTQKVNS VISHGSNKVG PHSTQLQWVT GPWLACSRTC DTGWHTRTVQ CQDGNRKLAK GCLLSQRPSA FKQCLLKKC. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Cleaves aggrecan, a cartilage proteoglycan, and may be involved in its turnover. May play an important role in the destruction of aggrecan in arthritic diseases. May play a role in proteolytic processing mostly during the peri-implantation period. Reference: "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."The MGC Project Team Genome Res. 14:2121-2127(2004). Function: Metalloproteinase that plays an important role in connective tissue organization, development, inflammation, arthritis, and cell migration. ADAMTS5 is an extracellular matrix (ECM) degrading enzyme that show proteolytic activity toward the hyalectan group of chondroitin sulfate proteoglycans (CSPGs) including aggrecan, versican, brevican and neurocan. Cleavage within the hyalectans occurs at Glu-Xaa recognition motifs. Plays a role in embryonic development, including limb and cardiac morphogenesis, and skeletal muscle development through its versican remodeling properties. Participates in the development of brown adipose tissue and browning of white adipose tissue