60 kDa chaperonin (groL), Yersinia pestis, recombinant

Organism: Yersinia pestis (strain Pestoides F). Source: E.coli. Expression Region: 1-548aa. Protein Length: Full Length. Tag Info: N-terminal 6xHis-SUMO-tagged. Target Protein Sequence: MAAKDVKFGN DARIKMLRGV NILADAVKVT LGPKGRNVVL DKSFGSPTIT KDGVSVAREI ELEDKFENMG AQMVKEVASK ANDAAGDGTT TATVLAQSII TEGLKAVAAG MNPMDLKRGI DKAVIAAVEE LKKLSVPCSD SKAIAQVGTI SANSDSTVGE LIAQAMEKVG KEGVITVEEG SGLQDELDVV EGMQFDRGYL SPYFINKPET GSIELESPFI LLADKKISNI REMLPVLEAV AKAGKPLLII AEDVEGEALA TLVVNTMRGI VKVAAVKAPG FGDRRKAMLQ DIATLTAGTV ISEEIGLELE KTTLEDLGQA KRVVINKDTT IIIDGVGDEA AIQGRVAQIR QQIEDATSDY DKEKLQERVA KLAGGVAVIK VGAATEVEMK EKKARVEDAL HATRAAVEEG VVAGGGVALI RAAHAIAGLK GDNEDQNVGI KVALRAMESP LRQIVVNAGE EASVIANKVK AGEGSFGYNA YTEEYGDMIA MGILDPTKVT RSALQYAASI AGLMITTECM VTDLPRDDKG ADMGAGGMGG MGGMGGMM. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Prevents misfolding and promotes the refolding and proper assbly of unfolded polypeptides generated under stress conditions. Reference: Complete sequence of chromosome of Yersinia pestis Pestoides F.US DOE Joint Genome InstituteCopeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Di Bartolo G., Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F. , Land M., Hauser L., Worsham P., Chu M., Bearden S., Garcia E., Richardson P. Function: Prevents misfolding and promotes the refolding and proper assembly of unfolded polypeptides generated under stress conditions.