50S ribosomal protein L2 (rplB), Mycobacterium tuberculosis, recombinant

Organism: Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv). Source: E.coli. Expression Region: 1-280aa. Protein Length: Full Length. Tag Info: N-terminal 6xHis-tagged. Target Protein Sequence: MAIRKYKPTT PGRRGASVSD FAEITRSTPE KSLVRPLHGR GGRNAHGRIT TRHKGGGHKR AYRMIDFRRN DKDGVNAKVA HIEYDPNRTA RIALLHYLDG EKRYIIAPNG LSQGDVVESG ANADIKPGNN LPLRNIPAGT LIHAVELRPG GGAKLARSAG SSIQLLGKEA SYASLRMPSG EIRRVDVRCR ATVGEVGNAE QANINWGKAG RMRWKGKRPS VRGVVMNPVD HPHGGGEGKT SGGRHPVSPW GKPEGRTRNA NKSSNKFIVR RRRTGKKHSR. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: One of the primary rRNA binding proteins. Required for association of the 30S and 50S subunits to form the 70S ribosome, for tRNA binding and peptide bond formation. It has been suggested to have peptidyltransferase activity, this is somewhat controversial. Makes several contacts with the 16S rRNA in the 70S ribosome. Reference: "Structural insights into species-specific features of the ribosome from the human pathogen Mycobacterium tuberculosis." Yang K., Chang J.Y., Cui Z., Li X., Meng R., Duan L., Thongchol J., Jakana J., Huwe C.M., Sacchettini J.C., Zhang J. Nucleic Acids Res 45:10884-10894(2017). Function: nan