5'-Nucleotidase/5'-NT/CD73 (AAH65937.1, C-Fc) (recombinant human)

Activity: Immobilized Human CD73-Fc(Cat#PKSH033885) at 2µg/ml (100 µl/well) can bind Anti-Human CD73 mAb* .*: Biotinylated by NHS-biotin prior to testing. The ED50 of Anti-Human CD73 mAb* is 0.31 ug/ml. Protein Construction: Recombinant Human 5'-Nucleotidase is produced by our Mammalian expression system and the target gene encoding Trp27-Lys547 is expressed with a Fc tag at the C-terminus. Sequence: Trp27-Lys547. Fusion tag: C-Fc Endotoxin: < 1.0 EU per µg as determined by the LAL method. Apparent Molecular Mass: 85-100 kDa. Protein function: CD73 is a glycosyl phosphatidylinositol (GPI) anchored membrane protein that belongs to the 5'-nucleotidase family. CD73 is an ecto 5'Nucleotidase expressed by most cell types. CD73 hydrolyzes extracellular nucleotides into membrane permeable nucleosides. CD73 is one of several enzymes responsible for the production of extracellular adenosine, a signaling molecule that is involved in responses to inflammation and tissue injury. CD73 is a lymphocyte maturation marker that has functions independent of its catalytic activity. CD73 is also a regulator of leukocyte extravasation, a function that requires its 5'Nucleotidase activity. Defects in NT5E are the cause of calcification of joints and arteries (CAJA). The recombinant CD73 lacking GPI anchor is secreted as a monomer.