5'-AMP-activated protein kinase subunit gamma-2 (PRKAG2), human, recombinant

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 1-328aa. Protein Length: Full Length of Isoform B. Tag Info: N-terminal 6xHis-tagged. Target Protein Sequence: MLEKLEFEDE AVEDSESGVY MRFMRSHKCY DIVPTSSKLV VFDTTLQVKK AFFALVANGV RAAPLWESKK QSFVGMLTIT DFINILHRYY KSPMVQIYEL EEHKIETWRE LYLQETFKPL VNISPDASLF DAVYSLIKNK IHRLPVIDPI SGNALYILTH KRILKFLQLF MSDMPKPAFM KQNLDELGIG TYHNIAFIHP DTPIIKALNI FVERRISALP VVDESGKVVD IYSKFDVINL AAEKTYNNLD ITVTQALQHR SQYFEGVVKC NKLEILETIV DRIVRAEVHR LVVVNEADSI VGIISLSDIL QALILTPAGA KQKETETE. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: AMP/ATP-binding subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton, probably by indirectly activating myosin. Gamma non-catalytic subunit mediates binding to AMP, ADP and ATP, leading to activate or inhibit AMPK: AMP-binding results in allosteric activation of alpha catalytic subunit (PRKAA1 or PRKAA2) both by inducing phosphorylation and preventing dephosphorylation of catalytic subunits. ADP also stimulates phosphorylation, without stimulating already phosphorylated catalytic subunit. ATP promotes dephosphorylation of catalytic subunit, rendering the AMPK enzyme inactive. Reference: "CBS domains form energy-sensing modules whose binding of adenosine ligands is disrupted by disease mutations."Scott J.W., Hawley S.A., Green K.A., Anis M., Stewart G., Scullion G.A., Norman D.G., Hardie D.G.J. Clin. Invest. 113:274-284(2004). Function: AMP/ATP-binding subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes