4,5-DOPA dioxygenase extradiol (DODA), Portulaca grandiflora, recombinant

Organism: Portulaca grandiflora (Rose moss). Source: E.coli. Expression Region: 1-271aa. Protein Length: Full Length. Tag Info: N-terminal 10xHis-SUMO-tagged and C-terminal Myc-tagged. Target Protein Sequence: MGVGKEVSFK ESFFLSHGNP AMLADESFIA RNFLLGWKKN VFPVKPKSIL VVSAHWETDV PCVSAGQYPN VIYDFTEVPA SMFQMKYPAP GCPKLAKRVQ ELLIAGGFKS AKLDEERGFD HSSWVPLSMM CPEADIPVCQ LSVQPGLDAT HHFNVGRALA PLKGEGVLFI GSGGAVHPSD DTPHWFDGVA PWAAEFDQWL EDALLEGRYE DVNNYQTKAP EGWKLAHPIP EHFLPLHVAM GAGGEKSKAE LIYRTWDHGT LGYASYKFTS I. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Opens the cyclic ring of dihydroxy-phenylalanine (DOPA) between carbons 4 and 5, thus producing an unstable seco-DOPA that rearranges nonenzymatically to betalamic acid. Reference: "Characterization and functional identification of a novel plant 4,5-extradiol dioxygenase involved in betalain pigment biosynthesis in Portulaca grandiflora." Christinet L., Burdet F.X., Zaiko M., Hinz U.G., Zryd J.-P. Plant Physiol. 134:265-274(2004). Function: Opens the cyclic ring of dihydroxy-phenylalanine (DOPA) between carbons 4 and 5, thus producing an unstable seco-DOPA that rearranges nonenzymatically to betalamic acid.