3-Phosphoshikimate 1-carboxyvinyltransferase (aroA), Salmonella typhimurium, recombinant

Organism: Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720). Source: E.coli. Expression Region: 1-427aa. Protein Length: Full Length. Tag Info: N-terminal 10xHis-tagged and C-terminal Myc-tagged. Target Protein Sequence: MESLTLQPIA RVDGAINLPG SKSVSNRALL LAALACGKTA LTNLLDSDDV RHMLNALSAL GINYTLSADR TRCDITGNGG ALRAPGALEL FLGNAGTAMR PLAAALCLGQ NEIVLTGEPR MKERPIGHLV DSLRQGGANI DYLEQENYPP LRLRGGFTGG DIEVDGSVSS QFLTALLMTA PLAPKDTIIR VKGELVSKPY IDITLNLMKT FGVEIANHHY QQFVVKGGQQ YHSPGRYLVE GDASSASYFL AAGAIKGGTV KVTGIGRKSM QGDIRFADVL EKMGATITWG DDFIACTRGE LHAIDMDMNH IPDAAMTIAT TALFAKGTTT LRNIYNWRVK ETDRLFAMAT ELRKVGAEVE EGHDYIRITP PAKLQHADIG TYNDHRMAMC FSLVALSDTP VTILDPKCTA KTFPDYFEQL ARMSTPA. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Catalyzes the transfer of the enolpyruvyl moiety of phosphoenolpyruvate (PEP) to the 5-hydroxyl of shikimate-3-phosphate to produce enolpyruvyl shikimate-3-phosphate and inorganic phosphate. Reference: "Complete genome sequence of Salmonella enterica serovar Typhimurium LT2." McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P., Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D., Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E. Wilson R.K. Nature 413:852-856(2001). Function: nan