3-Phosphoshikimate 1-carboxyvinyltransferase 1,chloroplastic (EPSPS-1), Nicotiana tabacum, recombina

Organism: Nicotiana tabacum(Common tobacco). Source: E.coli. Expression Region: 75-518aa. Protein Length: Full Length of Mature Protein. Tag Info: N-terminal 10xHis-tagged. Target Protein Sequence: KPNEIVLQPI KDISGTVKLP GSKSLSNRIL LLAALSKGRT VVDNLLSSDD IHYMLGALKT LGLHVEDDNE NQRAIVEGCG GQFPVGKKSE EEIQLFLGNA GTAMRPLTAA VTVAGGHSRY VLDGVPRMRE RPIGDLVDGL KQLGAEVDCF LGTNCPPVRI VSKGGLPGGK VKLSGSISSQ YLTALLMAAP LALGDVEIEI IDKLISVPYV EMTLKLMERF GVSVEHTSSW DKFLVRGGQK YKSPGKAYVE GDASSASYFL AGAAVTGGTV TVEGCGTSSL QGDVKFAEVL EKMGAEVTWT ENSVTVKGPP RNSSGMKHLR AVDVNMNKMP DVAMTLAVVA LFADGPTAIR DVASWRVKET ERMIAICTEL RKLGATVVEG SDYCIITPPE KLNVTEIDTY DDHRMAMAFS LAACADVPVT IKDPGCTRKT FPNYFDVLQQ YSKH. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Catalyzes the transfer of the enolpyruvyl moiety of phosphoenolpyruvate to the 5-hydroxyl of shikimate-3-phosphate to produce enolpyruvyl shikimate-3-phosphate and inorganic phosphate. Reference: "Expression and stability of amplified genes encoding 5-enolpyruvylshikimate-3-phosphate synthase in glyphosate-tolerant tobacco cells." Wang Y., Jones J., Weller S., Goldsbrough P.B. Plant Mol. Biol. 17:1127-1138(1991). Function: nan