3-Oxo-5-alpha-steroid 4-dehydrogenase 2 (SRD5A2), partial, human, recombinant

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 29-71aa. Protein Length: Partial. Tag Info: N-terminal 6xHis-KSI-tagged. Target Protein Sequence: KPSGYGKHTE SLKPAATRLP ARAAWFLQEL PSFAVPAGIL ARQ. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Converts testosterone (T) into 5-alpha-dihydrotestosterone (DHT) and progesterone or corticosterone into their corresponding 5-alpha-3-oxosteroids. It plays a central role in sexual differentiation and androgen physiology. Reference: "Biochemical and pharmacogenetic dissection of human steroid 5 alpha-reductase type II." Makridakis N.M., di Salle E., Reichardt J.K. Pharmacogenetics 10:407-413(2000). Function: nan