2-5-Oligoadenylate synthase-like protein (OASL), human, recombinant

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 2-514aa. Protein Length: Full Length of Mature Protein. Tag Info: N-terminal 6xHis-tagged. Target Protein Sequence: ALMQELYSTP ASRLDSFVAQ WLQPHREWKE EVLDAVRTVE EFLRQEHFQG KRGLDQDVRV LKVVKVGSFG NGTVLRSTRE VELVAFLSCF HSFQEAAKHH KDVLRLIWKT MWQSQDLLDL GLEDLRMEQR VPDALVFTIQ TRGTAEPITV TIVPAYRALG PSLPNSQPPP EVYVSLIKAC GGPGNFCPSF SELQRNFVKH RPTKLKSLLR LVKHWYQQYV KARSPRANLP PLYALELLTI YAWEMGTEED ENFMLDEGFT TVMDLLLEYE VICIYWTKYY TLHNAIIEDC VRKQLKKERP IILDPADPTL NVAEGYRWDI VAQRASQCLK QDCCYDNREN PISSWNVKRA RDIHLTVEQR GYPDFNLIVN PYEPIRKVKE KIRRTRGYSG LQRLSFQVPG SERQLLSSRC SLAKYGIFSH THIYLLETIP SEIQVFVKNP DGGSYAYAIN PNSFILGLKQ QIEDQQGLPK KQQQLEFQGQ VLQDWLGLGI YGIQDSDTLI LSKKKGEALF PAS. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Does not have 2'-5'-OAS activity, but can bind double-stranded RNA. Displays antiviral activity against encephalomyocarditis virus (EMCV) and hepatitis C virus (HCV) via an alternative antiviral pathway independent of RNase L. Reference: "2',5'-Oligoadenylate synthetase-like gene highly induced by hepatitis C virus infection in human liver is inhibitory to viral replication in vitro." Ishibashi M., Wakita T., Esumi M. Biochem. Biophys. Res. Commun. 392:397-402(2010). Function: nan