1-Acylglycerol-3-phosphate O-acyltransferase ABHD5 (ABHD5), human, recombinant

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 2-349aa. Protein Length: Full Length of Mature Protein. Tag Info: N-terminal 6xHis-SUMO-tagged. Target Protein Sequence: AAEEEEVDSA DTGERSGWLT GWLPTWCPTS ISHLKEAEEK MLKCVPCTYK KEPVRISNGN KIWTLKFSHN ISNKTPLVLL HGFGGGLGLW ALNFGDLCTN RPVYAFDLLG FGRSSRPRFD SDAEEVENQF VESIEEWRCA LGLDKMILLG HNLGGFLAAA YSLKYPSRVN HLILVEPWGF PERPDLADQD RPIPVWIRAL GAALTPFNPL AGLRIAGPFG LSLVQRLRPD FKRKYSSMFE DDTVTEYIYH CNVQTPSGET AFKNMTIPYG WAKRPMLQRI GKMHPDIPVS VIFGARSCID GNSGTSIQSL RPHSYVKTIA ILGAGHYVYA DQPEEFNQKV KEICDTVD. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Lysophosphatidic acid acyltransferase which functions in phosphatidic acid biosynthesis. May regulate the cellular storage of triacylglycerol through activation of the phospholipase PNPLA2. Involved in keratinocyte differentiation. Reference: "Mutations in CGI-58, the gene encoding a new protein of the esterase/lipase/thioesterase subfamily, in Chanarin-Dorfman syndrome."Lefevre C., Jobard F., Caux F., Bouadjar B., Karaduman A., Heilig R., Lakhdar H., Wollenberg A., Verret J.-L., Weissenbach J., Oezguec M., Lathrop M., Prud'homme J.-F., Fischer J.Am. J. Hum. Genet. 69:1002-1012(2001). Function: Lysophosphatidic acid acyltransferase which functions in phosphatidic acid biosynthesis