Primers

Primers are short oligonucleotides, that usually consist of 20 to 40 nucleotides. In molecular biology, primers are mostly used for PCR (Polymerase Chain Reaction), that allows the amplification of DNA in vitro. The amplification is achieved by DNA-polymerases, to which the primers serve as starting points during DNA-synthesis. Primers can be used to obtain specific DNA-fragments for cloning experiments or, in the form of qRT-PCR (quantitative Real-Time PCR), for quantitative detection of nucleic acids.

Primers are short oligonucleotides, that usually consist of 20 to 40 nucleotides . In molecular biology, primers are mostly used for PCR (Polymerase Chain Reaction), that allows the... read more »
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Primers

Primers are short oligonucleotides, that usually consist of 20 to 40 nucleotides. In molecular biology, primers are mostly used for PCR (Polymerase Chain Reaction), that allows the amplification of DNA in vitro. The amplification is achieved by DNA-polymerases, to which the primers serve as starting points during DNA-synthesis. Primers can be used to obtain specific DNA-fragments for cloning experiments or, in the form of qRT-PCR (quantitative Real-Time PCR), for quantitative detection of nucleic acids.

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IL10, Human interleukin 10, Real Time PCR Primer Set
IL10, Human interleukin 10, Real Time PCR Primer Set

Item number: VHPS-4508

Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with water as needed prior to use. This amount is sufficient for 1000 x 20µl PCR reactions assuming a final primer concentration of 0.1 µM. Add cDNA template. Recommended...
Keywords: IL10, CSIF, IL-10, Interleukin-10, Cytokine synthesis inhibitory factor
Application: RNA quantification
45.00€ *
Review
COL1A1, Human collagen, type I, alpha 1, Real Time PCR Primer Set
COL1A1, Human collagen, type I, alpha 1, Real Time PCR...

Item number: VHPS-2103

Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with water as needed prior to use. This amount is sufficient for 1000 x 20µl PCR reactions assuming a final primer concentration of 0.1 µM. Add cDNA template. Recommended...
Keywords: COL1A1, Alpha-1 type I collagen, Collagen alpha-1(I) chain
Application: RNA quantification
45.00€ *
Review
CCL2, Human chemokine (C-C motif) ligand 2, Real Time PCR Primer Set
CCL2, Human chemokine (C-C motif) ligand 2, Real Time PCR...

Item number: VHPS-1614

Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with water as needed prior to use. This amount is sufficient for 1000 x 20µl PCR reactions assuming a final primer concentration of 0.1 µM. Add cDNA template. Recommended...
Keywords: CCL2, MCP1, MCAF, HC11, MCP-1, C-C motif chemokine 2, Small-inducible cytokine A2, Monocyte secretory protein JE, Monocyte...
Application: RNA quantification
45.00€ *
Review
HPRT1, Human hypoxanthine phosphoribosyltransferase 1, Real Time PCR Primer Set
HPRT1, Human hypoxanthine phosphoribosyltransferase 1,...

Item number: VHPS-4263

Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with water as needed prior to use. This amount is sufficient for 1000 x 20µl PCR reactions assuming a final primer concentration of 0.1 µM. Add cDNA template. Recommended...
Keywords: HPRT, HPRT1, HGPRT, HGPRTase, EC=2.4.2.8, Hypoxanthine-guanine phosphoribosyltransferase
Application: RNA quantification
45.00€ *
Review
B2M, Human beta-2-microglobulin, Real Time PCR Primer Set
B2M, Human beta-2-microglobulin, Real Time PCR Primer Set

Item number: VHPS-705

Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with water as needed prior to use. This amount is sufficient for 1000 x 20µl PCR reactions assuming a final primer concentration of 0.1 µM. Add cDNA template. Recommended...
Keywords: B2M, CDABP0092, Beta-2-microglobulin, Beta-2-microglobulin form pI 5.3
Application: RNA quantification
45.00€ *
Review
Il10, Mouse interleukin 10, Real Time PCR Primer Set
Il10, Mouse interleukin 10, Real Time PCR Primer Set

Item number: VMPS-3052

Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with water as needed prior to use. This amount is sufficient for 1000 x 20µl PCR reactions assuming a final primer concentration of 0.1 µM. Add cDNA template. Recommended...
Keywords: Il10, CSIF, IL-10, Interleukin-10, Cytokine synthesis inhibitory factor
Application: RNA quantification
45.00€ *
Review
Pgk1, Mouse phosphoglycerate kinase 1, Real Time PCR Primer Set
Pgk1, Mouse phosphoglycerate kinase 1, Real Time PCR...

Item number: VMPS-4697

Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with water as needed prior to use. This amount is sufficient for 1000 x 20µl PCR reactions assuming a final primer concentration of 0.1 µM. Add cDNA template. Recommended...
Keywords: Pgk1, EC=2.7.2.3, Phosphoglycerate kinase 1
Application: RNA quantification
45.00€ *
Review
Ccl2, Mouse chemokine (C-C motif) ligand 2, Real Time PCR Primer Set
Ccl2, Mouse chemokine (C-C motif) ligand 2, Real Time PCR...

Item number: VMPS-904

Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with water as needed prior to use. This amount is sufficient for 1000 x 20µl PCR reactions assuming a final primer concentration of 0.1 µM. Add cDNA template. Recommended...
Keywords: Je, Ccl2, MCP-1, C-C motif chemokine 2, Small-inducible cytokine A2, Monocyte chemotactic protein 1, Monocyte...
Application: RNA quantification
45.00€ *
Review
VIM, Human vimentin, Real Time PCR Primer Set
VIM, Human vimentin, Real Time PCR Primer Set

Item number: VHPS-9912

Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with water as needed prior to use. This amount is sufficient for 1000 x 20µl PCR reactions assuming a final primer concentration of 0.1 µM. Add cDNA template. Recommended...
Keywords: VIM, Vimentin
Application: RNA quantification
45.00€ *
Review
CCL8, Human chemokine (C-C motif) ligand 8, Real Time PCR Primer Set
CCL8, Human chemokine (C-C motif) ligand 8, Real Time PCR...

Item number: VHPS-1625

Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with water as needed prior to use. This amount is sufficient for 1000 x 20µl PCR reactions assuming a final primer concentration of 0.1 µM. Add cDNA template. Recommended...
Keywords: MCP2, CCL8, HC14, MCP-2, MCP-2(6-76), C-C motif chemokine 8, Small-inducible cytokine A8, Monocyte chemotactic protein 2,...
Application: RNA quantification
45.00€ *
Review
CDH1, Human cadherin 1, type 1, E-cadherin (epithelial), Real Time PCR Primer Set
CDH1, Human cadherin 1, type 1, E-cadherin (epithelial),...

Item number: VHPS-1738

Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with water as needed prior to use. This amount is sufficient for 1000 x 20µl PCR reactions assuming a final primer concentration of 0.1 µM. Add cDNA template. Recommended...
Keywords: CDH1, hCG_28201, Cadherin 1, type 1, E-cadherin (Epithelial), isoform CRA_b, cDNA, FLJ92924, Homo sapiens cadherin 1, type...
Application: RNA quantification
45.00€ *
Review
MMP2, Human matrix metallopeptidase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase), R
MMP2, Human matrix metallopeptidase 2 (gelatinase A,...

Item number: VHPS-5759

Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with water as needed prior to use. This amount is sufficient for 1000 x 20µl PCR reactions assuming a final primer concentration of 0.1 µM. Add cDNA template. Recommended...
Keywords: PEX, MMP2, CLG4A, MMP-2, TBE-1, EC=3.4.24.24, Gelatinase A, 72 kDa gelatinase, Matrix metalloproteinase-2, 72 kDa type IV...
Application: RNA quantification
45.00€ *
Review
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