Primers

Primers are short oligonucleotides, that usually consist of 20 to 40 nucleotides. In molecular biology, primers are mostly used for PCR (Polymerase Chain Reaction), that allows the amplification of DNA in vitro. The amplification is achieved by DNA-polymerases, to which the primers serve as starting points during DNA-synthesis. Primers can be used to obtain specific DNA-fragments for cloning experiments or, in the form of qRT-PCR (quantitative Real-Time PCR), for quantitative detection of nucleic acids.

Primers are short oligonucleotides, that usually consist of 20 to 40 nucleotides . In molecular biology, primers are mostly used for PCR (Polymerase Chain Reaction), that allows the... read more »

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Primers

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Bdnf, Rat brain derived neurotrophic factor, Real Time...

Item number: VRPS-628

Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with water as needed prior to use. This amount is sufficient for 1000 x 20µl PCR reactions assuming a final primer concentration of 0.1 µM. Add cDNA template. Recommended...

Keywords:	Bdnf, BDNF, Brain-derived neurotrophic factor
Application:	RNA quantification

54.00€ *

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Custom Rat PCR Primer Sets

Item number: CRPS-1

Please inform Biomol about the Unigene Symbol of Accession Number of the corresponding gene. Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with water as needed prior to use. This amount is sufficient for 1000 x 20µl...

Keywords:	PCR, gene expression analysis
Application:	RNA quantification

54.00€ *

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Human Caspase Gene Family Primer Sets

Item number: HGF-3

Includes validated primer sets for Human CASP1, CASP2, CASP3, CASP4, CASP5, CASP6, CASP7, CASP8, CASP9, CASP10, CASP14. Contains 20 µl (50 µM) of each primer set (For/Rev combined) in individual tubes. Perform up to 500 assays (based on 20 µl assay volume) for each gene.

Application:

RT-PCR, Gene expression analysis

180.00€ *

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Human Tumor Necrosis Factor (TNF) Gene Family Primer Sets

Item number: HGF-5

Includes validated primer sets for Human TNF, LTA, LTB, TNFSF4, TNFSF5, TNFSF6, TNFSF7, TNFSF8, TNFSF9, TNFSF10, TNFSF11, TNFSF13, TNFSF13B, TNFSF14, TNFSF15, TNFSF18. Contains 20 µl (50 µM) of each primer set (For/Rev combined) in individual tubes. Perform up to 500 assays (based on 20 µl assay volume) for each gene.

Application:

RT-PCR, Gene expression analysis

253.00€ *

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Human Hypoxia Signaling Primer Library

Item number: HHYP-1

Masterplate for SYBR green based Real Time PCR. Contains 88 primer sets directed against pathway-related genes and 8 housekeeping gene primer sets. Provided in a 96-well microplate (20 µl - 10 µM). Sufficient for 100 PCR arrays (based on 20 µl assay volume per reaction).

Keywords:	HHYP-I
Application:	Gene expression profiling, PCR array

896.00€ *

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Human Ligand Gated Ion Channels Primer Library

Item number: HLGI-1

Keywords:	HLGI-I
Application:	Gene expression profiling, PCR array

1,258.00€ *

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Human SMAD Signaling Primer Library

Item number: HSMAD-1

Masterplate for SYBR green based Real Time PCR. Contains 88 primer sets directed against SMAD signaling genes and 8 housekeeping gene primer sets. Provided in a 96-well microplate (20 µl - 10 µM). Perform up to 100 PCR arrays (based on 20 µl assay volume per reaction). Just add cDNA template and SYBR green master mix.

Keywords:	HSMAD-I
Application:	Gene expression profiling, PCR array

896.00€ *

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Human TGF Beta Signaling Primer Library

Item number: HTGFB-1

Masterplate for SYBR green based Real Time PCR. Contains 88 primer sets directed against TGF beta signaling genes and 8 housekeeping gene primer sets. Provided in a 96-well microplate (20 µl - 10 µM). Perform up to 100 PCR arrays (based on 20 µl assay volume per reaction). Just add cDNA template and SYBR green...

Keywords:	HTGFB-I
Application:	Gene expression profiling, PCR array

896.00€ *

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Human Tumor Invasion/Metastasis Primer Library

Item number: HTIM-1

Masterplate for SYBR green based Real Time PCR. Contains 88 primer sets directed against tumor invasion/metastasis genes and 8 housekeeping gene primer sets. Provided in a 96-well microplate (20 µl - 10 µM). Sufficient for 100 PCR arrays (based on 20 µl assay volume per reaction).

Keywords:	HTIM-I
Application:	Gene expression profiling, PCR array

714.00€ *

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Human Toll-like Receptor Signaling Primer Library

Item number: HTLR-1

Masterplate for SYBR green based Real Time PCR. Contains 88 primer sets directed against Toll-like Receptor signaling genes and 8 housekeeping gene primer sets. Provided in a 96-well microplate (20 µl - 10 µM). Sufficient for 100 PCR arrays (based on 20 µl assay volume per reaction).

Keywords:	HTLR-I, TLR
Application:	Gene expression profiling, PCR array

896.00€ *

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Mouse Angiogenesis Primer Library

Item number: MAPL-1

Masterplate for SYBR green based Real Time PCR. Contains 88 primer sets directed against angiogenesis genes and 8 housekeeping gene primer sets. Provided in a 96-well microplate (20 µl - 10 µM). Sufficient for 100 PCR arrays (based on 20 µl assay volume per reaction).

Keywords:	MAPL-I
Application:	Gene expression profiling, PCR array

714.00€ *

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Mouse Cytokine Primer Library II

Item number: MCA-2

Masterplate for SYBR green based Real Time PCR. Contains 88 primer sets directed against cytokine genes and 8 housekeeping gene primer sets. Provided in a 96-well microplate (20 µl - 10 µM). Sufficient for 100 PCR arrays (based on 20 µl assay volume per reaction).

Keywords:	MCA-II
Application:	Gene expression profiling, PCR array

714.00€ *

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