Primers

Primers are short oligonucleotides, that usually consist of 20 to 40 nucleotides. In molecular biology, primers are mostly used for PCR (Polymerase Chain Reaction), that allows the amplification of DNA in vitro. The amplification is achieved by DNA-polymerases, to which the primers serve as starting points during DNA-synthesis. Primers can be used to obtain specific DNA-fragments for cloning experiments or, in the form of qRT-PCR (quantitative Real-Time PCR), for quantitative detection of nucleic acids.

Primers are short oligonucleotides, that usually consist of 20 to 40 nucleotides . In molecular biology, primers are mostly used for PCR (Polymerase Chain Reaction), that allows the... read more »
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Primers

Primers are short oligonucleotides, that usually consist of 20 to 40 nucleotides. In molecular biology, primers are mostly used for PCR (Polymerase Chain Reaction), that allows the amplification of DNA in vitro. The amplification is achieved by DNA-polymerases, to which the primers serve as starting points during DNA-synthesis. Primers can be used to obtain specific DNA-fragments for cloning experiments or, in the form of qRT-PCR (quantitative Real-Time PCR), for quantitative detection of nucleic acids.

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Lor, Mouse loricrin, Real Time PCR Primer Set
Lor, Mouse loricrin, Real Time PCR Primer Set

Item number: VMPS-3536

Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with water as needed prior to use. This amount is sufficient for 1000 x 20µl PCR reactions assuming a final primer concentration of 0.1 µM. Add cDNA template. Recommended...
Keywords: Lor, Loricrin
Application: RNA quantification
45.00€ *
Review
Nos3, Mouse nitric oxide synthase 3, endothelial cell, Real Time PCR Primer Set
Nos3, Mouse nitric oxide synthase 3, endothelial cell,...

Item number: VMPS-4290

Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with water as needed prior to use. This amount is sufficient for 1000 x 20µl PCR reactions assuming a final primer concentration of 0.1 µM. Add cDNA template. Recommended...
Keywords: Nos3, eNOS, cNOS, Ecnos, EC-NOS, NOSIII, NOS type III, EC=1.14.13.39, Endothelial NOS, Constitutive NOS, Nitric oxide...
Application: RNA quantification
45.00€ *
Review
Ocln, Mouse occludin, Real Time PCR Primer Set
Ocln, Mouse occludin, Real Time PCR Primer Set

Item number: VMPS-4418

Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with water as needed prior to use. This amount is sufficient for 1000 x 20µl PCR reactions assuming a final primer concentration of 0.1 µM. Add cDNA template. Recommended...
Keywords: Ocln, Occludin, mCG_116163, Ocln protein, Occludin, isoform CRA_a
Application: RNA quantification
45.00€ *
Review
Pparg, Mouse peroxisome proliferator activated receptor gamma, Real Time PCR Primer Set
Pparg, Mouse peroxisome proliferator activated receptor...

Item number: VMPS-4942

Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with water as needed prior to use. This amount is sufficient for 1000 x 20µl PCR reactions assuming a final primer concentration of 0.1 µM. Add cDNA template. Recommended...
Keywords: Pparg, Pparg protein
Application: RNA quantification
45.00€ *
Review
Ptgs2, Mouse prostaglandin-endoperoxide synthase 2, Real Time PCR Primer Set
Ptgs2, Mouse prostaglandin-endoperoxide synthase 2, Real...

Item number: VMPS-5166

Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with water as needed prior to use. This amount is sufficient for 1000 x 20µl PCR reactions assuming a final primer concentration of 0.1 µM. Add cDNA template. Recommended...
Keywords: Ptgs2, PES-2, COX-2, PHS II, PGHS-2, Gripghs, EC=1.14.99.1, TIS10 protein, PGH synthase 2, Cyclooxygenase-2, Prostaglandin...
Application: RNA quantification
45.00€ *
Review
Axl, Mouse AXL receptor tyrosine kinase, Real Time PCR Primer Set
Axl, Mouse AXL receptor tyrosine kinase, Real Time PCR...

Item number: VMPS-562

Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with water as needed prior to use. This amount is sufficient for 1000 x 20µl PCR reactions assuming a final primer concentration of 0.1 µM. Add cDNA template. Recommended...
Keywords: Axl, Putative uncharacterized protein
Application: RNA quantification
45.00€ *
Review
Snca, Mouse synuclein, alpha, Real Time PCR Primer Set
Snca, Mouse synuclein, alpha, Real Time PCR Primer Set

Item number: VMPS-6150

Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with water as needed prior to use. This amount is sufficient for 1000 x 20µl PCR reactions assuming a final primer concentration of 0.1 µM. Add cDNA template. Recommended...
Keywords: Syn, NACP, Snca, Alpha-synuclein, Non-A beta component of AD amyloid, Non-A4 component of amyloid precursor
Application: RNA quantification
45.00€ *
Review
Bdnf, Mouse brain derived neurotrophic factor, Real Time PCR Primer Set
Bdnf, Mouse brain derived neurotrophic factor, Real Time...

Item number: VMPS-641

Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with water as needed prior to use. This amount is sufficient for 1000 x 20µl PCR reactions assuming a final primer concentration of 0.1 µM. Add cDNA template. Recommended...
Keywords: Bdnf, BDNF, Brain-derived neurotrophic factor
Application: RNA quantification
45.00€ *
Review
Tktl1, Mouse transketolase-like 1, Real Time PCR Primer Set
Tktl1, Mouse transketolase-like 1, Real Time PCR Primer Set

Item number: VMPS-6625

Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with water as needed prior to use. This amount is sufficient for 1000 x 20µl PCR reactions assuming a final primer concentration of 0.1 µM. Add cDNA template. Recommended...
Keywords: Tktl1, Transketolase-like 1
Application: RNA quantification
45.00€ *
Review
Col1A1, Rat collagen, type I, alpha 1, Real Time PCR Primer Set
Col1A1, Rat collagen, type I, alpha 1, Real Time PCR...

Item number: VRPS-1237

Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with water as needed prior to use. This amount is sufficient for 1000 x 20µl PCR reactions assuming a final primer concentration of 0.1 µM. Add cDNA template. Recommended...
Keywords: Col1a1, Alpha-1 type I collagen, Collagen alpha-1(I) chain
Application: RNA quantification
54.00€ *
Review
Creb1, Rat cAMP responsive element binding protein 1, Real Time PCR Primer Set
Creb1, Rat cAMP responsive element binding protein 1,...

Item number: VRPS-1319

Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with water as needed prior to use. This amount is sufficient for 1000 x 20µl PCR reactions assuming a final primer concentration of 0.1 µM. Add cDNA template. Recommended...
Keywords: Creb1, CREB-1, cAMP-responsive element-binding protein 1, Cyclic AMP-responsive element-binding protein 1
Application: RNA quantification
54.00€ *
Review
Mef2A, Rat In multiple Geneids, Real Time PCR Primer Set
Mef2A, Rat In multiple Geneids, Real Time PCR Primer Set

Item number: VRPS-3589

Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with water as needed prior to use. This amount is sufficient for 1000 x 20µl PCR reactions assuming a final primer concentration of 0.1 µM. Add cDNA template. Recommended...
Keywords: Mef2a, Myocyte-specific enhancer factor 2A
Application: RNA quantification
54.00€ *
Review
24 from 2073 pages