Primers

Primers are short oligonucleotides, that usually consist of 20 to 40 nucleotides. In molecular biology, primers are mostly used for PCR (Polymerase Chain Reaction), that allows the amplification of DNA in vitro. The amplification is achieved by DNA-polymerases, to which the primers serve as starting points during DNA-synthesis. Primers can be used to obtain specific DNA-fragments for cloning experiments or, in the form of qRT-PCR (quantitative Real-Time PCR), for quantitative detection of nucleic acids.

Primers are short oligonucleotides, that usually consist of 20 to 40 nucleotides . In molecular biology, primers are mostly used for PCR (Polymerase Chain Reaction), that allows the... read more »
Close window
Primers

Primers are short oligonucleotides, that usually consist of 20 to 40 nucleotides. In molecular biology, primers are mostly used for PCR (Polymerase Chain Reaction), that allows the amplification of DNA in vitro. The amplification is achieved by DNA-polymerases, to which the primers serve as starting points during DNA-synthesis. Primers can be used to obtain specific DNA-fragments for cloning experiments or, in the form of qRT-PCR (quantitative Real-Time PCR), for quantitative detection of nucleic acids.

12 from 2073 pages
No results were found for the filter!
Tfam, Mouse transcription factor A, mitochondrial, Real Time PCR Primer Set
Tfam, Mouse transcription factor A, mitochondrial, Real...

Item number: VMPS-6543

Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with water as needed prior to use. This amount is sufficient for 1000 x 20µl PCR reactions assuming a final primer concentration of 0.1 µM. Add cDNA template. Recommended...
Keywords: Tfam, Hmgts, mtTFA, TS-HMG, Transcription factor A, mitochondrial, Testis-specific high mobility group protein
Application: RNA quantification
45.00€ *
Review
Tgfb1, Mouse transforming growth factor, beta 1, Real Time PCR Primer Set
Tgfb1, Mouse transforming growth factor, beta 1, Real...

Item number: VMPS-6559

Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with water as needed prior to use. This amount is sufficient for 1000 x 20µl PCR reactions assuming a final primer concentration of 0.1 µM. Add cDNA template. Recommended...
Keywords: Tgfb1, mCG_7649, Transforming growth factor, beta 1
Application: RNA quantification
45.00€ *
Review
Il1A, Rat interleukin 1 alpha, Real Time PCR Primer Set
Il1A, Rat interleukin 1 alpha, Real Time PCR Primer Set

Item number: VRPS-2928

Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with water as needed prior to use. This amount is sufficient for 1000 x 20µl PCR reactions assuming a final primer concentration of 0.1 µM. Add cDNA template. Recommended...
Keywords: Il1a, IL-1 alpha, Interleukin-1 alpha
Application: RNA quantification
54.00€ *
Review
Ppia, Rat Peptidylprolyl isomerase A (cyclophilin A), Real Time PCR Primer Set
Ppia, Rat Peptidylprolyl isomerase A (cyclophilin A),...

Item number: VRPS-6988

Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with water as needed prior to use. This amount is sufficient for 1000 x 20µl PCR reactions assuming a final primer concentration of 0.1 µM. Add cDNA template. Recommended...
Keywords: p31, Ppia, p1B15, PPIase A, Rotamase A, EC=5.2.1.8, Cyclophilin A, Cyclosporin A-binding protein, Peptidyl-prolyl...
Application: RNA quantification
54.00€ *
Review
Human Apoptosis Primer Library
Human Apoptosis Primer Library

Item number: HPA-1

Masterplate for SYBR green based Real Time PCR. Contains 88 primer sets directed against apoptosis genes and 8 housekeeping gene primer sets. Provided in a 96-well microplate (20 µl - 10 µM). Sufficient for 100 PCR arrays (based on 20 µl assay volume per reaction).
Keywords: HPA-I
Application: Gene expression profiling, PCR array
714.00€ *
Review
CAT, Human catalase, Real Time PCR Primer Set
CAT, Human catalase, Real Time PCR Primer Set

Item number: VHPS-1541

Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with water as needed prior to use. This amount is sufficient for 1000 x 20µl PCR reactions assuming a final primer concentration of 0.1 µM. Add cDNA template. Recommended...
Keywords: CAT, Catalase, EC=1.11.1.6
Application: RNA quantification
45.00€ *
Review
DMP1, Human dentin matrix acidic phosphoprotein 1, Real Time PCR Primer Set
DMP1, Human dentin matrix acidic phosphoprotein 1, Real...

Item number: VHPS-2650

Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with water as needed prior to use. This amount is sufficient for 1000 x 20µl PCR reactions assuming a final primer concentration of 0.1 µM. Add cDNA template. Recommended...
Keywords: DMP1, DMP-1, Dentin matrix protein 1, Dentin matrix acidic phosphoprotein 1
Application: RNA quantification
45.00€ *
Review
DSC2, Human desmocollin 2, Real Time PCR Primer Set
DSC2, Human desmocollin 2, Real Time PCR Primer Set

Item number: VHPS-2744

Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with water as needed prior to use. This amount is sufficient for 1000 x 20µl PCR reactions assuming a final primer concentration of 0.1 µM. Add cDNA template. Recommended...
Keywords: DSC2, CDHF2, Desmocollin-3, Desmocollin-2, Cadherin family member 2, Desmosomal glycoprotein II, Desmosomal glycoprotein III
Application: RNA quantification
45.00€ *
Review
ALB, Human albumin, Real Time PCR Primer Set
ALB, Human albumin, Real Time PCR Primer Set

Item number: VHPS-287

Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with water as needed prior to use. This amount is sufficient for 1000 x 20µl PCR reactions assuming a final primer concentration of 0.1 µM. Add cDNA template. Recommended...
Keywords: ALB, Serum albumin
Application: RNA quantification
45.00€ *
Review
GSTM1, Human glutathione S-transferase mu 1, Real Time PCR Primer Set
GSTM1, Human glutathione S-transferase mu 1, Real Time...

Item number: VHPS-3897

Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with water as needed prior to use. This amount is sufficient for 1000 x 20µl PCR reactions assuming a final primer concentration of 0.1 µM. Add cDNA template. Recommended...
Keywords: GST1, GTH4, GSTM1, GSTM1-1, GSTM1a-1a, GSTM1b-1b, EC=2.5.1.18, GST class-mu 1, GST HB subunit 4, Glutathione S-transferase...
Application: RNA quantification
45.00€ *
Review
GSTM3, Human glutathione S-transferase mu 3 (brain), Real Time PCR Primer Set
GSTM3, Human glutathione S-transferase mu 3 (brain), Real...

Item number: VHPS-3898

Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with water as needed prior to use. This amount is sufficient for 1000 x 20µl PCR reactions assuming a final primer concentration of 0.1 µM. Add cDNA template. Recommended...
Keywords: GST5, GSTM3, GSTM3-3, hGSTM3-3, EC=2.5.1.18, GST class-mu 3, Glutathione S-transferase Mu 3
Application: RNA quantification
45.00€ *
Review
HLA-DRA, Human major histocompatibility complex, class II, DR alpha, Real Time PCR Primer Set
HLA-DRA, Human major histocompatibility complex, class...

Item number: VHPS-4159

Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with water as needed prior to use. This amount is sufficient for 1000 x 20µl PCR reactions assuming a final primer concentration of 0.1 µM. Add cDNA template. Recommended...
Keywords: MHC class II antigen DRA, HLA class II histocompatibility antigen, DR alpha chain
Application: RNA quantification
45.00€ *
Review
12 from 2073 pages