Primers

Primers are short oligonucleotides, that usually consist of 20 to 40 nucleotides. In molecular biology, primers are mostly used for PCR (Polymerase Chain Reaction), that allows the amplification of DNA in vitro. The amplification is achieved by DNA-polymerases, to which the primers serve as starting points during DNA-synthesis. Primers can be used to obtain specific DNA-fragments for cloning experiments or, in the form of qRT-PCR (quantitative Real-Time PCR), for quantitative detection of nucleic acids.

Primers are short oligonucleotides, that usually consist of 20 to 40 nucleotides . In molecular biology, primers are mostly used for PCR (Polymerase Chain Reaction), that allows the... read more »
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Primers

Primers are short oligonucleotides, that usually consist of 20 to 40 nucleotides. In molecular biology, primers are mostly used for PCR (Polymerase Chain Reaction), that allows the amplification of DNA in vitro. The amplification is achieved by DNA-polymerases, to which the primers serve as starting points during DNA-synthesis. Primers can be used to obtain specific DNA-fragments for cloning experiments or, in the form of qRT-PCR (quantitative Real-Time PCR), for quantitative detection of nucleic acids.

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Gapdh, Rat glyceraldehyde phosphate dehydrogenase (Gapd), Real Time PCR Primer Set
Gapdh, Rat glyceraldehyde phosphate dehydrogenase (Gapd),...

Item number: VRPS-6986

Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with water as needed prior to use. This amount is sufficient for 1000 x 20µl PCR reactions assuming a final primer concentration of 0.1 µM. Add cDNA template. Recommended...
Keywords: Gapd, Gapdh, GAPDH, BARS-38, EC=1.2.1.12, EC=2.6.99.-, Peptidyl-cysteine S-nitrosylase GAPDH, Glyceraldehyde-3-phosphate...
Application: RNA quantification
54.00€ *
Review
Human Ferroptosis PCR Primer Library
Human Ferroptosis PCR Primer Library

Item number: HFER-1

Masterplate for SYBR green based Real Time PCR. Contains 88 primer sets directed against pathway-related genes and 8 housekeeping gene primer sets. Provided in a 96-well microplate (20 µl - 10 µM). Sufficient for 100 PCR arrays (based on 20 µl assay volume per reaction).
Keywords: HFER-I
Application: Gene expression profiling, PCR array
896.00€ *
Review
Mouse Autophagy Primer Library
Mouse Autophagy Primer Library

Item number: MATPL-1

Masterplate for SYBR green based Real Time PCR. Contains 88 primer sets directed against autophagy genes and 8 housekeeping gene primer sets. Provided in a 96-well microplate (20 µl - 10 µM). Sufficient for 100 PCR arrays (based on 20 µl assay volume per reaction).
Keywords: MATPL-I
Application: Gene expression profiling, PCR array
714.00€ *
Review
CCL5, Human chemokine (C-C motif) ligand 5, Real Time PCR Primer Set
CCL5, Human chemokine (C-C motif) ligand 5, Real Time PCR...

Item number: VHPS-1623

Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with water as needed prior to use. This amount is sufficient for 1000 x 20µl PCR reactions assuming a final primer concentration of 0.1 µM. Add cDNA template. Recommended...
Keywords: CCL5, hCG_32592, Chemokine ligand 5, Chemokine (C-C motif) ligand 5
Application: RNA quantification
45.00€ *
Review
CDKN1A, Human cyclin-dependent kinase inhibitor 1A (p21, Cip1), Real Time PCR Primer Set
CDKN1A, Human cyclin-dependent kinase inhibitor 1A (p21,...

Item number: VHPS-1770

Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with water as needed prior to use. This amount is sufficient for 1000 x 20µl PCR reactions assuming a final primer concentration of 0.1 µM. Add cDNA template. Recommended...
Keywords: p21, CAP20, MDA-6, CDKN1A, CDK-interacting protein 1, Cyclin-dependent kinase inhibitor 1, Melanoma...
Application: RNA quantification
45.00€ *
Review
CLDN1, Human claudin 1, Real Time PCR Primer Set
CLDN1, Human claudin 1, Real Time PCR Primer Set

Item number: VHPS-1999

Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with water as needed prior to use. This amount is sufficient for 1000 x 20µl PCR reactions assuming a final primer concentration of 0.1 µM. Add cDNA template. Recommended...
Keywords: CLD1, CLDN1, Claudin-1, Senescence-associated epithelial membrane protein
Application: RNA quantification
45.00€ *
Review
CLDN2, Human claudin 2, Real Time PCR Primer Set
CLDN2, Human claudin 2, Real Time PCR Primer Set

Item number: VHPS-2008

Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with water as needed prior to use. This amount is sufficient for 1000 x 20µl PCR reactions assuming a final primer concentration of 0.1 µM. Add cDNA template. Recommended...
Keywords: SP82, CLDN2, PSEC0059, Claudin-2
Application: RNA quantification
45.00€ *
Review
AFP, Human alpha-fetoprotein, Real Time PCR Primer Set
AFP, Human alpha-fetoprotein, Real Time PCR Primer Set

Item number: VHPS-217

Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with water as needed prior to use. This amount is sufficient for 1000 x 20µl PCR reactions assuming a final primer concentration of 0.1 µM. Add cDNA template. Recommended...
Keywords: AFP, HPAFP, Alpha-fetoprotein, Alpha-fetoglobulin, Alpha-1-fetoprotein
Application: RNA quantification
45.00€ *
Review
CS, Human citrate synthase, Real Time PCR Primer Set
CS, Human citrate synthase, Real Time PCR Primer Set

Item number: VHPS-2250

Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with water as needed prior to use. This amount is sufficient for 1000 x 20µl PCR reactions assuming a final primer concentration of 0.1 µM. Add cDNA template. Recommended...
Keywords: CS, EC=2.3.3.1, Citrate synthase, mitochondrial
Application: RNA quantification
45.00€ *
Review
CXCL2, Human chemokine (C-X-C motif) ligand 2, Real Time PCR Primer Set
CXCL2, Human chemokine (C-X-C motif) ligand 2, Real Time...

Item number: VHPS-2354

Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with water as needed prior to use. This amount is sufficient for 1000 x 20µl PCR reactions assuming a final primer concentration of 0.1 µM. Add cDNA template. Recommended...
Keywords: HSF, GRO2, CXCL2, Gro-beta, SB-251353, MIP2-alpha, GRO-beta-T, GRO-beta(5-73), C-X-C motif chemokine 2, Growth-regulated...
Application: RNA quantification
45.00€ *
Review
DPP4, Human dipeptidyl-peptidase 4, Real Time PCR Primer Set
DPP4, Human dipeptidyl-peptidase 4, Real Time PCR Primer Set

Item number: VHPS-2720

Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with water as needed prior to use. This amount is sufficient for 1000 x 20µl PCR reactions assuming a final primer concentration of 0.1 µM. Add cDNA template. Recommended...
Keywords: CD26, DPP4, ADCP2, ADABP, TP103, ADCP-2, DPP IV, EC=3.4.14.5, Dipeptidyl peptidase 4, Dipeptidyl peptidase IV, T-cell...
Application: RNA quantification
45.00€ *
Review
DSG2, Human desmoglein 2, Real Time PCR Primer Set
DSG2, Human desmoglein 2, Real Time PCR Primer Set

Item number: VHPS-2756

Primers are provided as a 40 µl solution containing both primers at a final concentration of 50 µM in 10 mM Tris-HCl (pH 7.5), 0.1 mM EDTA. Dilute with water as needed prior to use. This amount is sufficient for 1000 x 20µl PCR reactions assuming a final primer concentration of 0.1 µM. Add cDNA template. Recommended...
Keywords: DSG2, HDGC, CDHF5, Desmoglein-2, Cadherin family member 5
Application: RNA quantification
45.00€ *
Review
10 from 2073 pages