VPS4B (Vector Vector will be determined during the manufacturing process, either pENTR223.1 or pUC,

Length: 1335 Sequence: atgtcatcca cttcgcccaa cctccagaaa gcgatagatc tggctagcaa agcagcgcaa gaagacaagg ctgggaacta cgaagaagcc cttcagctct atcagcatgc tgtgcagtat tttcttcatg tcgttaaata tgaagcacag ggtgataaag ccaagcaaag tatcagggca aagtgtacag aatatcttga tagagcagaa aaactaaagg agtacctgaa aaataaagag aaaaaagcac agaagccagt gaaagaagga cagccgagtc cagcagatga gaaggggaat gacagtgatg gggaaggaga atctgatgat cctgaaaaaa agaaactaca gaatcaactt caaggtgcca ttgttataga acgaccaaat gtgaaatgga gtgacgttgc tggacttgaa ggagccaaag aagcactgaa agaggctgtg atactgccta ttaaatttcc tcatcttttt acaggcaaga gaacaccttg gaggggaatc ctattatttg ggccgcctgg aacaggaaag tcctacttag ccaaagctgt agcaacagaa gccaacaact caacattttt ttcaatatct tcctctgatc ttgtttctaa gtggctaggt gaaagtgaaa aactggttaa gaatttattc caacttgcca gagagaacaa gccctccatt atcttcattg atgaaattga ttctctctgt ggttcaagaa gtgaaaatga aagtgaagcc gcacgtagaa ttaagacgga gttcctagtg caaatgcaag gggttggtgt agacaatgat ggaattttgg ttctgggagc tacaaatata ccctgggttc tggattctgc cattaggcga agatttgaga aacgaattta tattcccttg ccggaacccc atgcccgagc agcaatgttt aaactgcacc tagggaccac tcagaacagt ctcacggaag cagactttcg ggaacttggg aggaaaacag atggttattc aggggcagat ataagtatca ttgtacgtga tgcccttatg cagcctgtta ggaaagtaca gtcagctact cattttaaaa aggttcgcgg accttcccga gctgatccta accatcttgt agatgatctg ctaacacctt gctctccagg tgaccctggt gccattgaaa tgacatggat ggatgtccct ggagataaac ttttggagcc agttgtttcc atgtcggata tgttgcggtc actatctaac acaaaaccta cagtcaatga acatgacttg ttgaaattaa agaagtttac agaagatttt ggtcaagaag Protein function: Involved in late steps of the endosomal multivesicular bodies (MVB) pathway. Recognizes membrane-associated ESCRT-III assemblies and catalyzes their disassembly, possibly in combination with membrane fission. Redistributes the ESCRT-III components to the cytoplasm for further rounds of MVB sorting. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. In conjunction with the ESCRT machinery also appears to function in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and enveloped virus budding (HIV-1 and other lentiviruses). VPS4A/B are required for the exosomal release of SDCBP, CD63 and syndecan (PubMed:22660413). [The UniProt Consortium]