USP44 (Vector pENTR223.1, Accession No. BC030704)

Length: 2139 Sequence: atgctagcaa tggatacgtg caaacatgtt gggcagctgc agcttgctca agaccattcc agcctcaacc ctcagaaatg gcactgtgtg gactgcaaca cgaccgagtc catttgggct tgccttagct gctcccatgt tgcctgtgga agatatattg aagagcatgc actcaagcac tttcaagaaa gcagtcatcc tgttgcattg gaggtgaatg agatgtacgt tttttgttac ctttgtgatg attatgttct gaatgataac gcaactggag acctgaagtt actacgacgt acattaagtg ccatcaaaag tcaaaattat cactgcacaa ctcgtagtgg gaggttttta cggtccatgg gtacaggtga tgattcttat ttcttacatg acggtgccca atctctgctt caaagtgaag atcaactgta tactgctctt tggcacagga gaaggatact aatgggtaaa atctttcgaa catggtttga acaatcaccc attggaagaa aaaagcaaga agaaccattt caggaaaaaa tagtagtaaa aagagaagta aagaaaagac ggcaggaatt ggagtatcaa gttaaagcag aattggaaag tatgcctcca agaaagagtt tacgtttaca agggctcgct cagtcgacca taatagaaat agtttctgtt caggtgccag cacaaacgcc agcatcacca gcaaaagata aagtactctc tacctcagaa aatgaaatat ctcaaaaagt cagtgactcc tcagttaaac gaaggccaat agtaactcct ggtgtaacag gattgagaaa tttgggaaat acttgctata tgaattctgt tcttcaggta ttgagtcatt tacttatttt tcgacaatgt tttttaaagc ttgatctgaa ccaatggctg gctatgactg ctagcgagaa gacaagatct tgtaagcatc caccagtcac agatacagta gtatatcaaa tgaatgaatg tcaggaaaaa gatacaggtt ttgtttgctc cagacaatca agtctgtcat caggactaag tggtggagca tcaaaaggta gaaagatgga acttattcag ccaaaggagc caacttcaca gtacatttct ctttgtcatg aattgcatac tttgttccaa gtcatgtggt ctggaaagtg ggcgttggtc tcaccatttg ctatgctaca ctcagtgtgg agactcattc ctgcctttcg tggttacgcc caacaagacg ctcaggaatt tctttgtgaa cttttagata aaatacaacg tgaattagag acaactggta ccagtttacc agctcttatc cccacttctc aaaggaaact catcaaacaa gttctgaatg ttgtaaataa catttttcat ggacaacttc ttagtcaggt tacatgtctt gcatgtgaca acaaatcaaa taccatagaa cctttctggg acttgtcatt ggagtttcca gaaaggtatc aatgcagtgg aaaagatatt gcttcccagc catgtctggt tactgaaatg ttggccaaat ttacagaaac tgaagcttta gaaggaaaaa tctacgtatg tgaccagtgt aactcaaagc gtagaaggtt ttcctccaaa ccagttgtac tcacagaagc ccagaaacaa cttatgatat gccacctacc tcaggttctc agactgcacc tcaaacgatt caggtggtca ggacgtaata accgagagaa gattggtgtt catgttggct ttgaggaaat cttaaacatg gagccctatt gctgcaggga gaccctgaaa tccctcagac cagaatgctt tatctatgac ttgtccgcgg tggtgatgca ccatgggaaa ggatttggct cagggcacta cactgcctac tgctataatt ctgaaggagg gttctgggta cactgcaatg attccaaact aagcatgtgc actatggacg aagtatgcaa ggctcaagct tatatcttgt tttataccca acgagttact gagaatggac attctaaact tttgcctcca gagctcctgt tggggagcca acatcccaat gaagacgctg atacctcgtc taatgaaatc Protein function: Deubiquitinase that plays a key regulatory role in the spindle assembly checkpoint or mitotic checkpoint by preventing premature anaphase onset. Acts by specifically mediating deubiquitination of CDC20, a negative regulator of the anaphase promoting complex/cyclosome (APC/C). Deubiquitination of CDC20 leads to stabilize the MAD2L1-CDC20-APC/C ternary complex (also named mitotic checkpoint complex), thereby preventing premature activation of the APC/C. Promotes association of MAD2L1 with CDC20 and reinforces the spindle assembly checkpoint. Acts as a negative regulator of histone H2B (H2BK120ub1) ubiquitination. [The UniProt Consortium]