UPF3B (Vector pUC, Accession No. BC121017)

Length: 1413 Sequence: ATGAAGGAAG AGAAGGAGCA CAGGCCTAAG GAGAAGCGAG TAACCCTGTT AACCCCCGCC GGGGCCACAG GCAGCGGTGG TGGGACCTCG GGGGACAGCT CCAAGGGGGA AGATAAGCAG GATCGCAACA AGGAGAAGAA AGAAGCGCTG AGCAAGGTGG TAATTCGAAG ATTACCTCCC ACTTTGACCA AGGAGCAGCT TCAGGAACAT CTTCAACCTA TGCCTGAGCA TGATTATTTT GAGTTTTTTT CTAATGATAC GAGTTTGTAT CCTCATATGT ATGCCAGAGC ATACATCAAC TTTAAAAACC AAGAGGACAT TATTTTGTTC AGGGATCGCT TTGATGGTTA TGTATTCCTT GACAATAAAG GTCAGGAATA TCCCGCTATA GTAGAATTTG CACCTTTTCA AAAAGCTGCA AAAAAGAAGA CTAAGAAAAG AGATACCAAA GTCGGGACTA TCGATGATGA TCCAGAATAT AGAAAGTTTT TGGAAAGTTA TGCCACAGAT AATGAGAAAA TGACATCTAC TCCAGAGACA CTGCTAGAGG AAATAGAAGC AAAAAATAGA GAATTAATAG CTAAAAAGAC AACCCCACTT TTGAGCTTCC TGAAAAACAA GCAGAGAATG AGAGAAGAAA AGAGAGAAGA AAGGAGGAGG AGAGAAATAG AAAGAAAAAG ACAAAGAGAA GAAGAGAGGA GGAAATGGAA AGAAGAAGAG AAACGAAAAA GGAAAGATAT AGAAAAGCTA AAGAAGATAG ACAGAATTCC AGAAAGGGAC AAATTAAAGG ATGAACCAAA GATTAAGCTG CTCAAGAAGC CAGAAAAAGG AGATGAAAAA GAATTGGACA AAAGAGAAAA AGCCAAGAAA TTGGACAAAG AGAATCTCAG TGATGAAAGA GCCAGTGGGC AAAGTTGTAC ATTGCCCAAG CGTTCTGATA GCGAACTTAA AGATGAAAAA CCAAAGAGAC CTGAAGATGA GAGCGGCAGA GACTATAGGG AGAGGGAACG GGAATATGAA CGAGATCAGG AGCACATACT TCGAGAAAGA GAGAGGCTGA AGCGGCAAGA AGAAGAGCGC CGTAGGCAGA AGGAGCGCTA TGAGAAAGAG AAGACTTTTA AGAGAAAAGA AGAAGAAATG AAAAAAGAGA AAGACACACT TCGGGATAAA GGAAAGAAGG CTGAAAGTAC AGAATCAATA GGCAGCTCAG AAAAAACTGA AAAGAAAGAA GAAGTGGTCA AGAGAGATCG AATAAGAAAC AAGGATCGTC CAGCGATGCA GCTTTACCAA CCAGGAGCTC GAAGCCGAAA TCGACTCTGT CCCCCTGATG ACAGCACCAA GTCTGGAGAT TCAGCAGCAG AAAGGAAGCA GGAAAGTGGT ATTAGCCATA GAAAAGAAGG AGGAGAGGAG Protein function: Involved in nonsense-mediated decay (NMD) of mRNAs containing premature stop codons by associating with the nuclear exon junction complex (EJC) and serving as link between the EJC core and NMD machinery. Recruits UPF2 at the cytoplasmic side of the nuclear envelope and the subsequent formation of an UPF1-UPF2-UPF3 surveillance complex (including UPF1 bound to release factors at the stalled ribosome) is believed to activate NMD. In cooperation with UPF2 stimulates both ATPase and RNA helicase activities of UPF1. Binds spliced mRNA upstream of exon-exon junctions. In vitro, stimulates translation, the function is independent of association with UPF2 and components of the EJC core. [The UniProt Consortium]