STT3B (Vector Vector will be determined during the manufacturing process, either pENTR223.1 or pUC,

Length: 678 Sequence: atgtcttggt gggattatgg ctatcagata gctggaatgg ctaatagaac tacgttggtg gataataaca cctggaataa cagccacata gcactggtgg gaaaagctat gtcttctaat gaaacagcag cctataaaat catgaggact ctagatgtag attatgtttt ggttattttt ggaggggtta ttggctattc tggtgatgat atcaacaaat ttctctggat ggttaggata gctgaaggag aacatcccaa agacattcgg gaaagtgact attttacccc acagggagaa ttccgtgtag acaaagcagg atcccctact ttgttgaatt gccttatgta taaaatgtca tactacagat ttggagaaat gcagctggat tttcgtacac ccccaggttt tgaccgaaca cgtaatgctg agattggaaa taaggacatt aaattcaaac atttggaaga agcctttaca tcagaacact ggcttgttag gatatataaa gtaaaagcac ctgataacag ggagacatta gatcacaaac ctcgagtcac caacattttc ccaaaacaga agtatttgtc aaagaagact accaaaagga agcgtggcta cattaaaaat aagctggttt ttaagaaagg caagaaaata tttaagaaga Protein function: Catalytic subunit of the oligosaccharyl transferase (OST) complex that catalyzes the initial transfer of a defined glycan (Glc(3)Man(9)GlcNAc(2) in eukaryotes) from the lipid carrier dolichol- pyrophosphate to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains, the first step in protein N-glycosylation. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). All subunits are required for a maximal enzyme activity. This subunit contains the active site and the acceptor peptide and donor lipid-linked oligosaccharide (LLO) binding pockets. STT3B is present in a small subset of OST complexes and mediates both cotranslational and post-translational N- glycosylation of target proteins: STT3B-containing complexes are required for efficient post-translational glycosylation and while they are less competent than STT3A-containing complexes for cotranslational glycosylation, they have the ability to mediate glycosylation of some nascent sites that are not accessible for STT3A. STT3B-containing complexes also act post-translationally and mediate modification of skipped glycosylation sites in unfolded proteins. Plays a role in ER- associated degradation (ERAD) pathway that mediates ubiquitin-dependent degradation of misfolded endoplasmic reticulum proteins by mediating N- glycosylation of unfolded proteins, which are then recognized by the ERAD pathway and targeted for degradation. Mediates glycosylation of the disease variant AMYL-TTR 'Asp-38' of TTR at 'Asn-118', leading to its degradation (PubMed:19167329, PubMed:22607976). [The UniProt Consortium]