SETD2 (Vector pUC, Accession No. BC117162)

Length: 6186 Sequence: atggaaagaa gaggcaagta ttcttcaaaa ctagaaagag aatctaaaag gacttcagaa aatgaagcaa ttaaaagatg ttgttctccc cctaatgaac tgggattccg acgagggtca tcatattcta agcatgacag tagtgcttcc cgttataaat ctaccctttc aaaacctata cccaagtctg ataaatttaa aaattctttc tgttgtacag aattaaatga agaaatcaaa cagtctcatt cttttagttt acagacacct tgttcaaaag gtagtgaatt aagaatgatt aataaaaatc ctgaaagaga aaaggctggg tctccagctc catcaaatcg attaaatgat tcacctactt taaaaaagct agatgaattg cctattttta agtccgaatt tataacacat gatagccatg atagtattaa ggaattagac tctttatcta aagtgaagaa tgatcaatta agaagttttt gtcccataga attaaatata aatggatctc ctggggcaga atctgatttg gcaacatttt gcacttctaa aactgatgct gttttaatga cttctgatga tagtgtgact ggatcggaat tatccccttt ggtcaaagca tgcatgcttt catcaaatgg atttcagaat attagtaggt gcaaagaaaa agacttggat gatacctgca tgctgcataa gaagtcagaa agcccattta gagaaacaga acctctggtg tcaccacacc aagataaact catgtctatg ccagttatga ctgtggatta ttccaaaaca gtagttaaag aaccagttga tacgagggtt tcttgctgca aaaccaaaga ttcagacata tactgtactt tgaacgatag caacccttct ttgtgtaact ctgaagctga aaatattgag ccttcagtta tgaagatttc ttcaaatagc tttatgaatg tgcatttgga atcaaaacca gttatatgtg atagtagaaa tttgacagat cactcaaaat ttgcatgtga agaatataag cagagcatcg gtagcactag ttcagcttct gttaatcatt ttgatgattt atatcaacct attgggagtt caggtattgc ttcatctctt cagagtcttc caccaggaat aaaggtggac agtctaactc tcttgaaatg cggagagaac acatctccag ttctggatgc agtgctaaag agtaaaaaaa gttcagagtt tttaaagcat gcagggaaag aaacaatagt agaagtaggt agtgaccttc ctgattcagg aaagggattt gcttccaggg agaacaggcg taataatggg ttatctggga aatgtttgca agaggctcaa gaagaaggga attccatatt gcctgaaaga agaggaagac cagaaatctc tttagatgaa agaggagaag gaggacatgt gcatacttct gatgactcag aagttgtatt ttcttcttgt gatttgaatt taaccatgga agacagtgat ggtgtaactt atgcattaaa gtgtgacagt agtggtcatg ccccagaaat tgtgtctaca gttcatgaag attattctgg ctcttctgaa agttcaaatg atgaaagtga ttcagaagat acagattcgg atgatagcag tattccaaga aaccgtctcc agtctgttgt ggttgtgcca aagaattcta ctttgcccat ggaagaaaca agtccttgtt cttctcggag cagtcaaagt tatagacact attctgacca ttgggaagat gagagattgg agtcaaggag acatttgtat gaggaaaaat ttgaaagtat agcaagtaaa gcctgtcctc aaactgataa gtttttcctt cataaaggaa cagagaagaa tccggaaatt tcttttacac agtccagtag aaaacaaata gataaccgcc tgcctgaact ttctcatcct cagagtgatg gggttgatag tacaagtcat acagatgtga aatctgaccc tctgggtcac ccaaattcag aggaaaccgt gaaagccaaa ataccttcta ggcagcaaga agagctgcca atttattctt ctgattttga agatgtccca aataagtctt ggcaacagac cactttccaa aacaggccag atagtagact gggaaaaaca gaattgagtt tttcttcctc ttgtgagata ccacatgtgg atggcttgca ctcatcagaa gagctcagaa acttaggttg ggacttctct caagaaaagc cttctaccac gtatcagcaa cctgacagta gctatggagc ttgtggtgga cacaagtatc agcaaaatgc agaacagtat ggtgggacac gtgattactg gcaaggcaat ggttactggg atccaagatc aggtagacct cctggaactg gggttgtgta tgatcgaact caaggacaag taccagattc cctaacagat gatcgtgaag aagaggagaa ttgggatcaa caggatggat cccatttttc agaccagtcc gataaatttc ttctatccct tcagaaagac aaggggtcag tgcaagcacc tgaaataagc agcaattcca ttaaggacac tttagctgtg aatgaaaaga aagatttttc aaaaaactta gaaaaaaatg atatcaaaga tagagggcct cttaaaaaaa ggaggcagga aatagagagt gattctgaaa gtgatggtga gcttcaggac agaaagaaag ttagagtgga ggtagagcag ggagagacat cagtgccccc aggttcagca ctggttgggc cctcctgtgt catggatgac ttcagggacc cacagcgatg gaaggaatgt gccaagcaag ggaaaatgcc atgttacttt gatcttattg aagaaaatgt ttatttaaca gaaagaaaga agaataaatc tcatcgagat attaagcgaa tgcagtgtga gtgtacacct ctttctaaag atgaaagagc tcaaggtgaa atagcatgtg gggaagattg tcttaatcgt cttctcatga ttgaatgttc ttctcggtgt ccaaatgggg attattgttc caatagacgg tttcagagaa aacagcatgc agatgtggaa gtcatactca cagaaaagaa aggctggggc ttgagagctg ccaaagacct tccttcgaac acctttgtcc tagaatattg tggagaggta ctcgatcata aagagtttaa agctcgagtg aaggagtatg cacgaaacaa aaacatccat tactatttca tggccctgaa gaatgatgag ataatagatg ccactcaaaa aggaaattgc tctcgtttca tgaatcacag ctgtgaacca aattgtgaaa cccaaaaatg gactgtgaac ggacaactga gggttgggtt ttttaccacc aaactggttc cttcaggctc agagttaacg tttgactatc agttccagag atatggaaaa gaagcccaga aatgtttctg cggatcagcc aattgccggg gttacctggg aggagaaaac agagtcagca tcagagcagc aggagggaaa atgaagaagg aacgatctcg taagaaggat tcagtggatg gagagctaga agctctgatg gaaaatggtg agggtctctc tgataaaaac caggtgctca gcttatcccg gctaatggtt agaattgaaa ctttggagca gaaacttacc tgtctggaac tcatacagaa cacacactca cagtcctgcc tgaagtcctt tctggaacgt catgggctgt ctttgttgtg gatctggatg gcagagctag gtgacggccg ggaaagtaac cagaagcttc aggaagagat tataaagact ttggaacact tgcccattcc tactaaaaat atgttggagg aaagcaaagt acttccaatt attcaacgct ggtctcagac taagactgct gtccctccgt tgagtgaagg agatgggtat tctagtgaga atacatcgcg tgctcataca ccactcaaca cacctgatcc ttccaccaag ctgagcacag aagctgacac agacactccc aagaaactaa tgtttcgcag actgaaaatt ataagtgaaa atagcatgga cagtgcaatc tctgatgcaa ccagtgagct agaaggcaag gatggcaaag aggatcttga tcaattagaa aatgtccctg tagaggaaga ggaagaattg cagtcacaac agctactccc acaacagctg cctgaatgca aagttgatag tgaaaccaac atagaagcta gtaagctacc tacatctgaa ccagaagctg acgctgaaat agagctcaaa gagagcaacg gcacaaaact agaagaacct attaatgaag aaacaccatc ccaagatgaa gaggagggtg tgtctgatgt ggagagtgaa aggagccaag aacagccaga taaaacagtg gatataagtg atttggccac caaactcctg gacagttgga aagacctaaa ggaggtatat cgaattccaa agaaaagtca aactgaaaag gaaaacacaa caactgaacg aggaagggat gctgttggct tcagagatca aacacctgcc ccgaagactc ctaataggtc aagagagaga gacccagaca agcaaactca aaataaagag aaaaggaaac gaagaagctc cctctcacca ccctcttctg cctatgagcg gggaacaaaa aggccagatg acagatatga tacaccaact tctaaaaaga aagtacgaat taaagaccgc aataaacttt ctacagagga acgccggaag ttgtttgagc aagaggtggc tcaacgggag gctcagaaac aacagcaaca gatgcagaac ctgggaatga catcaccact gccctatgac tctcttggtt ataatgcccc gcatcatccc tttgctggtt acccaccagg ttatcccatg caggcctatg tggatcccag caaccctaat gctggaaagg tgctcctgcc cacacccagc atggacccag tgtgttctcc tgctccttat gatcatgctc agcccttggt gggacattct acagaacccc tttctgcccc tccaccagta ccagtggtgc cacatgtggc agctcctgtg gaagtttcca gttcccagta tgtggcccag agtgatggtg tagtacacca agactccagc gttgctgtct tgccagtgcc ggcccccggc ccagttcagg gacagaatta tagtgtttgg gattcaaacc aacagtctgt cagtgtacag cagcagtact ctcctgcaca gtctcaagca accatatatt atcaaggaca gacatgtcca acagtctatg gtgtgacatc accttattca cagacaactc caccaattgt acagagttat gcccagccaa gtcttcagta tatccagggg caacagattt tcacagctca tccacaagga gtggtggtac agccagccgc agcagtgact acaatagttg caccagggca gcctcagccc ttgcagccat ctgaaatggt tgtgacaaat aatctcttgg atctgccgcc cccctctcct cccaaaccaa aaaccattgt cttacctccc aactggaaga cagctcgaga tccagaaggg aagatttatt actaccatgt gatcacaagg cagactcagt gggatcctcc tacttgggaa agcccaggag atgatgccag ccttgagcat gaagctgaga tggacctggg aactccaaca tatgatgaaa accccatgaa ggcctcgaaa aagcccaaga cagcagaagc agacacctcc agtgaactag caaagaaaag caaagaagta ttcagaaaag agatgtccca gttcatcgtc cagtgcctga acccttaccg gaaacctgac tgcaaagtgg gaagaattac cacaactgaa gactttaaac atctggctcg caagctgact cacggtgtta tgaataagga gctgaagtac tgtaagaatc ctgaggacct ggagtgcaat gagaatgtga aacacaaaac caaggagtac attaagaagt acatgcagaa gtttggggct gtttacaaac ccaaagagga cactgaatta Protein function: Histone methyltransferase that specifically trimethylates 'Lys-36' of histone H3 (H3K36me3) using dimethylated 'Lys-36' (H3K36me2) as substrate (PubMed:16118227, PubMed:19141475, PubMed:21526191, PubMed:21792193, PubMed:23043551, PubMed:27474439). It is capable of trimethylating unmethylated H3K36 (H3K36me0) in vitro (PubMed:19332550). Represents the main enzyme generating H3K36me3, a specific tag for epigenetic transcriptional activation. Plays a role in chromatin structure modulation during elongation by coordinating recruitment of the FACT complex and by interacting with hyperphosphorylated POLR2A (PubMed:23325844). Acts as a key regulator of DNA mismatch repair in G1 and early S phase by generating H3K36me3, a mark required to recruit MSH6 subunit of the MutS alpha complex: early recruitment of the MutS alpha complex to chromatin to be replicated allows a quick identification of mismatch DNA to initiate the mismatch repair reaction (PubMed:23622243). Required for DNA double-strand break repair in response to DNA damage: acts by mediating formation of H3K36me3, promoting recruitment of RAD51 and DNA repair via homologous recombination (HR) (PubMed:24843002). Acts as a tumor suppressor (PubMed:24509477). H3K36me3 also plays an essential role in the maintenance of a heterochromatic state, by recruiting DNA methyltransferase DNMT3A (PubMed:27317772). H3K36me3 is also enhanced in intron-containing genes, suggesting that SETD2 recruitment is enhanced by splicing and that splicing is coupled to recruitment of elongating RNA polymerase (PubMed:21792193). Required during angiogenesis. Required for endoderm development by promoting embryonic stem cell differentiation toward endoderm: acts by mediating formation of H3K36me3 in distal promoter regions of FGFR3, leading to regulate transcription initiation of FGFR3. In addition to histones, also mediates methylation of other proteins, such as tubulins and STAT1 (PubMed:27518565, PubMed:28753426). Trimethylates 'Lys-40' of alpha-tubulins such as TUBA1B (alpha- TubK40me3), alpha-TubK40me3 is required for normal mitosis and cytokinesis and may be a specific tag in cytoskeletal remodeling (PubMed:27518565). Involved in interferon-alpha-induced antiviral defense by mediating both monomethylation of STAT1 at 'Lys-525' and catalyzing H3K36me3 on promoters of some interferon-stimulated genes (ISGs) to activate gene transcription (PubMed:28753426). [The UniProt Consortium]