SCAP (Vector Vector will be determined during the manufacturing process, either pENTR223.1 or pUC, A

Length: 2718 Sequence: atgaccctga ctgaaaggct gcgtgagaag atatctcggg ccttctacaa ccatgggctc ctctgtgcat cctatcccat ccccatcatc ctcatcggct acttcaccct agtgcccgcc atccaggagt tctgtctctt tgctgtcgtg gggctggtgt ctgacttctt ccttcagatg ctgtttttca ccactgtcct gtccattgac attcgccgga tggagctagc agacctgaac aagcgactgc cccctgaggc ctgcctgccc tcagccaagc cagtggggca gccaacgcgc tacgagcggc agctggctgt gaggccgtcc acaccccaca ccatcacgtt gcagccgtct tccttccgaa acctgcggct ccccaagagg ctgcgtgttg tctacttcct ggcccgcacc cgcctggcac agcgcctcat catggctggc accgttgtct ggattggcat cctggtatac acagacccag cagggctgcg caactacctc gctgcccagg tgacggaaca gagcccattg ggtgagggag ccctggctcc catgcccgtg cctagtggca tgctgccccc cagccacccg gaccctgcct tctccatctt cccacctgat gcccctaagc tacctgagaa ccagacgtcg ccaggcgagt cacctgagcg tggaggtcca gcagaggttg tccatgacag cccagtccca gaggtaacct gggggcctga ggatgaggaa ctttggagga aattgtcctt ccgccactgg ccgacgctct tcagctatta caacatcaca ctggccaaga ggtacatcag cctgctgccc gtcatcccag tcacgctccg cctgaacccg agggaggctc tggagggccg gcaccctcag gacggccgca gtgcctggcc cccaccgggg cccatacctg ctgggcactg ggaagcagga cccaagggcc caggtggggt gcaggcccat ggagacgtca cgctgtacaa ggtggcggcg ctgggcctgg ccaccggcat cgtcttggtg ctgctgctgc tctgcctcta ccgcgtgcta tgcccgcgca actacgggca gctgggtggt gggcccgggc ggcggaggcg cggggagctg ccctgcgacg actacggcta tgcgccaccc gagacggaga tcgtgccgct tgtgctgcgc ggccacctca tggacatcga gtgcctggcc agcgacggca tgctgctggt gagctgctgc ctggcaggcc acatctgcgt gtgggacgcg cagaccgggg attgcctaac gcgcattccg cgcccagggc agcgccggga cagtggcgtg ggcagcgggc ttgaggctca ggagagctgg gaacgacttt cagatggtgg gaaggctggt ccagaggagc ctggggacag ccctcccctg agacaccgcc cccggggccc tccgccgcct tccctcttcg gggaccagcc tgacctcacc tgcttaattg acaccaactt ttcagcgcag cctcggtcct cacagcccac tcagcccgag ccccggcacc gggcggtctg tggccgctct cgggactccc caggctatga cttcagctgc ctggtgcagc gggtgtacca ggaggagggg ctggcggccg tctgcacacc agccctgcgc ccaccctcgc ctgggccggt gctgtcccag gcccctgagg acgagggtgg ctcccccgag aaaggctccc cttccctcgc ctgggccccc agtgccgagg gttccatctg gagcttggag ctgcagggca acctcatcgt ggtggggcgg agcagcggcc ggctggaggt gtgggacgcc attgaagggg tgctgtgctg cagcagcgag gaggtctcct caggcattac cgctctggtg ttcttggaca aaaggattgt ggctgcacgg ctcaacggtt cccttgattt cttctccttg gagacccaca ctgccctcag ccccctgcag tttagaggga ccccagggcg gggcagttcc cctgcctctc cagtgtacag cagcagcgac acagtggcct gtcacctgac ccacacagtg ccctgtgcac accaaaaacc catcacagcc ctgaaagccg ctgctgggcg cttggtgact gggagccaag accacacact gagagtgttc cgtctggagg actcgtgctg cctcttcacc cttcagggcc actcaggggc catcacgacc gtgtacattg accagaccat ggtgctggcc agtggaggac aagatggggc catctgcctg tgggatgtac tgactggcag ccgggtcagc catgtgtttg ctcaccgtgg ggatgtcacc tcccttacct gtaccacctc ctgtgtcatc agcagtggcc tggatgacct catcagcatc tgggaccgca gcacaggcat caagttctac tccattcagc aggacctggg ctgtggtgca agcttgggtg tcatctcaga caacctgctg gtgactggcg gccagggctg tgtctccttt tgggacctaa actacgggga cctgttacag acagtctacc tggggaagaa cagtgaggcc cagcctgccc gccagatcct ggtgctggac aacgctgcca ttgtctgcaa ctttggcagt gagctcagcc tggtgtatgt gccctctgtg ctggagaagc Protein function: Escort protein required for cholesterol as well as lipid homeostasis. Regulates export of the SCAP-SREBP complex from the endoplasmic reticulum to the Golgi upon low cholesterol, thereby regulating the processing of sterol regulatory element-binding proteins (SREBPs) SREBF1/SREBP1 and SREBF2/SREBP2. At high sterol concentrations, formation of a ternary complex with INSIG (INSIG1 or INSIG2) leads to mask the ER export signal in SCAP, promoting retention of the complex in the endoplasmic reticulum. Low sterol concentrations trigger release of INSIG, a conformational change in the SSD domain of SCAP, unmasking of the ER export signal, promoting recruitment into COPII-coated vesicles and transport of the SCAP-SREBP to the Golgi: in the Golgi, SREBPs are then processed, releasing the transcription factor fragment of SREBPs from the membrane, its import into the nucleus and up-regulation of LDLR, INSIG1 and the mevalonate pathway. Binds cholesterol via its SSD domain. [The UniProt Consortium]