RNF146 (Vector Vector will be determined during the manufacturing process, either pENTR223.1 or pUC,

Length: 1077 Sequence: atggctggct gtggtgaaat tgatcattca ataaacatgc ttcctacaaa caggaaagcg aacgagtcct gttctaatac tgcaccttct ttaaccgtcc ctgaatgtgc catttgtctg caaacatgtg ttcatccagt cagtctgccc tgtaagcacg ttttctgcta tctatgtgta aaaggagctt catggcttgg aaagcggtgt gctctttgtc gacaagaaat tcccgaggat ttccttgaca agccaacctt gttgtcacca gaagaactca aggcagcaag tagaggaaat ggtgaatatg catggtatta tgaaggaaga aatgggtggt ggcagtacga tgagcgcact agtagagagc tggaagatgc tttttccaaa ggtaaaaaga acactgaaat gttaattgct ggctttctgt atgtcgctga tcttgaaaac atggttcaat ataggagaaa tgaacatgga cgtcgcagga agattaagcg agatataata gatataccaa agaagggagt agctggactt aggctagact gtgatgctaa taccgtaaac ctagcaagag agagctctgc tgacggagcg gacagtgtat cagcacagag tggagcttct gttcagcccc tagtgtcttc tgtaaggccc ctaacatcag tagatggtca gttaacaagc cctgcaacac catcccctga tgcaagcact tctctggaag actcttttgc tcatttacaa ctcagtggag acaacacagc tgaaaggagt cataggggag aaggagaaga agatcatgaa tcaccatctt caggcagggt accagcacca gacacctcca ttgaagaaac tgaatcagat gccagtagtg atagtgagga tgtatctgca gttgttgcac agcactcctt gacccaacag agacttttgg tttctaatgc aaaccagaca gtacccgatc gatcagatcg atcgggaact gatcgatcag tagcaggggg tggaacagtg agtgtcagtg tcagatctag aaggcctgat ggacagtgca cagtaactga Protein function: E3 ubiquitin-protein ligase that specifically binds poly-ADP- ribosylated (PARsylated) proteins and mediates their ubiquitination and subsequent degradation. May regulate many important biological processes, such as cell survival and DNA damage response. Acts as an activator of the Wnt signaling pathway by mediating the ubiquitination of PARsylated AXIN1 and AXIN2, 2 key components of the beta-catenin destruction complex. Acts in cooperation with tankyrase proteins (TNKS and TNKS2), which mediate PARsylation of target proteins AXIN1, AXIN2, BLZF1, CASC3, TNKS and TNKS2. Recognizes and binds tankyrase-dependent PARsylated proteins via its WWE domain and mediates their ubiquitination, leading to their degradation. Different ubiquitin linkage types have been observed: TNKS2 undergoes ubiquitination at 'Lys-48' and 'Lys-63', while AXIN1 is only ubiquitinated at 'Lys-48'. May regulate TNKS and TNKS2 subcellular location, preventing aggregation at a centrosomal location. Neuroprotective protein. Protects the brain against N-methyl-D-aspartate (NMDA) receptor- mediated glutamate excitotoxicity and ischemia, by interfering with PAR-induced cell death, called parthanatos. Prevents nuclear translocation of AIFM1 in a PAR-binding dependent manner. Does not affect PARP1 activation. Protects against cell death induced by DNA damaging agents, such as N-methyl-N-nitro-N- nitrosoguanidine (MNNG) and rescues cells from G1 arrest. Promotes cell survival after gamma-irradiation. Facilitates DNA repair. [The UniProt Consortium]