RG9MTD1 (Vector Vector will be determined during the manufacturing process, either pENTR223.1 or pUC

Length: 1194 Sequence: atgagtgtta gtgtcaattt cttcagacct ttcaccaggt ttttggtgcc atttaccctt cataggaaga gaaataactt aacaattttg cagagataca tgtcttccaa aataccagct gttacttatc ctaaaaatga gagtacaccc ccttctgaag agctagagtt ggataagtgg aaaactacca tgaaatctag tgtgcaagaa gaatgtgttt caacaatctc aagcagtaag gatgaagatc ctctagctgc caccagagag ttcattgaga tgtggagatt gcttggcaga gaagtaccag aacacatcac tgaagaagag ctcaaaaccc ttatggaatg tgtttctaac acagcaaaaa aaaaatattt aaaatattta tatacgaagg aaaaagtgaa aaaagctagg caaataaaaa aggaaatgaa agcagcagca agggaagaag caaaaaatat caagctgcta gaaaccactg aggaagataa acagaaaaac tttctatttt tacgactttg ggataggaat atggacatag caatgggctg gaagggtgcc caggccatgc agtttggaca acctttggtt tttgacatgg cttacgaaaa ttatatgaaa cgaaaagaat tgcagaatac tgtttcccag cttttagaaa gtgaaggatg gaacagaaga aatgttgatc ctttccatat ttatttctgc aatctaaaaa tagatggtgc tttgcacaga gagttagtta aacggtatca agaaaaatgg gacaaattgc ttttaacatc aacagaaaag tctcatgtag atttatttcc aaaggacagt attatctatt taactgcaga ttctcccaat gttatgacta ctttcaggca tgacaaagtt tatgtaattg ggtcttttgt tgataagagt atgcagccag gcacatccct agccaaggca aaacggctga acctggcaac tgaatgcctt ccattagata aatatttaca atgggaaatt ggtaacaaaa atctcacctt agatcaaatg atacgtattt tgttatgtct gaaaaacaat ggtaattggc aagaggctct gcaattcgtt cccaagagaa aacatactgg ttttctggag atttctcagc attctcaaga gtttatcaac agactaaaga aggcaaagac Protein function: Mitochondrial tRNA N(1)-methyltransferase involved in mitochondrial tRNA maturation (PubMed:18984158, PubMed:21593607, PubMed:23042678, PubMed:27132592). Component of mitochondrial ribonuclease P, a complex composed of TRMT10C/MRPP1, HSD17B10/MRPP2 and PRORP/MRPP3, which cleaves tRNA molecules in their 5'-ends (PubMed:18984158). Together with HSD17B10/MRPP2, forms a subcomplex of the mitochondrial ribonuclease P, named MRPP1-MRPP2 subcomplex, which displays functions that are independent of the ribonuclease P activity (PubMed:23042678, PubMed:29040705). The MRPP1-MRPP2 subcomplex catalyzes the formation of N(1)-methylguanine and N(1)-methyladenine at position 9 (m1G9 and m1A9, respectively) in tRNAs, TRMT10C/MRPP1 acting as the catalytic N(1)-methyltransferase subunit (PubMed:23042678). The MRPP1-MRPP2 subcomplex also acts as a tRNA maturation platform: following 5'-end cleavage by the mitochondrial ribonuclease P complex, the MRPP1-MRPP2 subcomplex enhances the efficiency of 3'-processing catalyzed by ELAC2, retains the tRNA product after ELAC2 processing and presents the nascent tRNA to the mitochondrial CCA tRNA nucleotidyltransferase TRNT1 enzyme (PubMed:29040705). In addition to tRNA N(1)-methyltransferase activity, TRMT10C/MRPP1 also acts as a mRNA N(1)-methyltransferase by mediating methylation of adenosine residues at the N(1) position of MT-ND5 mRNA (PubMed:29072297). Associates with mitochondrial DNA complexes at the nucleoids to initiate RNA processing and ribosome assembly. [The UniProt Consortium]