RFWD3 (Vector pUC, Accession No. BC059371)

Length: 2250 Sequence: atggccagca gccaaggggg accagccctc ctccagcctg ttcctgctga tgtggtcagc agccaggggg taccatccat cctccagcca gctcctgctg aggtgatcag cagccaagcg acaccacccc tgctccagcc tgctccgcaa ctgtctgttg acctgacaga agtggaggtc ttgggagaag acactgtgga gaacatcaat ccaagaactt cagaacaaca taggcaggga tctgatggta atcacaccat cccagcatct tcgttgcatt caatgaccaa cttcatcagc ggactgcaga gacttcatgg catgctggaa ttcctgagac cttcatcttc aaaccacagt gtagggccaa tgagaacaag aaggagggta tctgcttcac ggagggcaag agccggaggg tctcagagga cagacagtgc caggttgaga gcaccattgg atgcttactt tcaggtgagc aggacccagc ctgacttgcc agctaccact tatgattcag agactaggaa tcctgtatct gaagagttgc aggtgtctag tagttctgat tctgacagtg acagctctgc agagtatgga ggggttgttg accaggcaga ggaatctgga gctgtcattt tagaagagca actagcaggt gtctcagcag agcaagaagt tacatgtatc gatggaggca agaccctccc caaacagcca tctccccaga agtctgagcc tctgctacct tctgcttcta tggatgagga agaaggggac acttgtacaa tatgtctgga acagtggacc aatgctgggg accaccggct ctcagcatta cgctgtgggc atctctttgg gtataggtgc atttccacgt ggcttaaagg acaagtacga aaatgtcccc agtgcaacaa gaaagccagg cacagtgaca ttgtcgtcct ttatgcccga accctgagag ctttggacac tagtgaacag gagcgcatga aaagttccct actgaaggaa cagatgctaa ggaaacaggc cgagttagaa tcagcacagt gccgactcca actgcaggtc ctcactgata agtgcactaa gcttcaaagg cgtgttcagg acttgcaaaa acttacgtca catcaaagtc agaatttaca gcaacccagg ggctcccaag catgggtcct gagctgctca ccctccagcc agggccagca caagcacaag taccacttcc aaaagacctt cacagtatct caggcaggaa actgccggat catggcatac tgtgatgctc tgagctgcct ggtgatatca cagccttctc ctcaggcctc ttttcttcca ggctttggtg ttaagatgtt gagtactgcc aacatgaaga gcagtcagta cattccgatg catggcaaac agatccgtgg actggcgttt agcagttacc tcagaggctt gctactctct gcttccctag acaacactat taaactgacc agcctggaga caaataccgt ggtccagact tataatgctg gacgtcctgt ctggagctgt tgctggtgtc ttgatgaggc taactacatc tatgctggac tggccaatgg ttcaattctg gtatatgacg tgcgaaacac gagcagtcat gtgcaggagt tagtagctca gaaagccaga tgcccactgg tctccctgtc atacatgccc agagctgcct cagctgcatt tccatatggt ggggtgctgg ctggaacctt ggaggatgct tcattctggg aacagaaaat ggacttttct cattggcctc atgtgctgcc cttggagcca gggggctgca tagactttca gacagagaac agctcccggc actgtcttgt gacctacagg cctgataaaa atcacaccac catacgaagt gtgctgatgg aaatgtccta ccgactggat gacactggaa atccaatctg ctcctgccag cctgtacata cattttttgg aggacctact tgcaaactat tgaccaaaaa tgccattttc caaagcccag agaatgatgg caacatcctg gtgtgtactg gggatgaagc agcaaattct gccctgctgt gggatgctgc cagtggctcg ttgctccagg acctacagac cgatcagcct gtgttggaca tctgcccatt tgaggtgaac cgtaacagct acttggctac cttaacagag aagatggtcc acacctataa Protein function: E3 ubiquitin-protein ligase required for the repair of DNA interstrand cross-links (ICL) in response to DNA damage (PubMed:21504906, PubMed:21558276, PubMed:26474068, PubMed:28575657, PubMed:28575658). Plays a key role in RPA-mediated DNA damage signaling and repair (PubMed:21504906, PubMed:21558276, PubMed:26474068, PubMed:28575657, PubMed:28575658, PubMed:28691929). Acts by mediating ubiquitination of the RPA complex (RPA1, RPA2 and RPA3 subunits) and RAD51 at stalled replication forks, leading to remove them from DNA damage sites and promote homologous recombination (PubMed:26474068, PubMed:28575657, PubMed:28575658). Also mediates the ubiquitination of p53/TP53 in the late response to DNA damage, and acts as a positive regulator of p53/TP53 stability, thereby regulating the G1/S DNA damage checkpoint (PubMed:20173098). May act by catalyzing the formation of short polyubiquitin chains on p53/TP53 that are not targeted to the proteasome (PubMed:20173098). In response to ionizing radiation, interacts with MDM2 and enhances p53/TP53 ubiquitination, possibly by restricting MDM2 from extending polyubiquitin chains on ubiquitinated p53/TP53 (PubMed:20173098). [The UniProt Consortium]