RC3H2 (Vector Vector will be determined during the manufacturing process, either pENTR223.1 or pUC,

Length: 1521 Sequence: atgcctgtgc aggcagctca atggacagaa tttctgtcct gtccaatctg ctataatgaa tttgatgaga atgtgcacaa acccatcagt ttaggttgtt cacacactgt ttgcaagacc tgcttgaata aacttcatcg aaaagcttgt ccttttgacc agactgccat caacacagat attgatgtac ttcctgtcaa cttcgcactt ctccagttag ttggagccca ggtaccagat catcagtcaa ttaagttaag taatctaggt gagaataaac actatgaggt tgcaaagaaa tgcgttgagg atttggcact ctacttaaaa ccactaagtg gaggtaaagg tgtagctagc ttgaaccaga gtgcactgag ccgtccaatg caaaggaaac tggtgacact tgtaaattgt caactggtgg aggaagaagg tcgtgtaaga gccatgcgag cagctcgttc ccttggagaa agaactgtaa cagaactgat attacagcac cagaaccctc agcagttgtc tgccaatcta tgggccgctg tcagggctcg aggatgccag tttttagggc cagctatgca agaagaggcc ttgaagctgg tgttactggc attagaagat ggttctgccc tctcaaggaa agttctggta ctttttgttg tgcagagact agaaccaaga tttcctcagg catcaaaaac aagtattggt catgttgtgc aactactgta tcgagcttct tgttttaagg ttaccaaaag agatgaagac tcttccctaa tgcagctgaa ggaggaattt cggagttatg aagcattacg cagagaacat gatgcccaaa ttgttcatat tgccatggaa gcaggactcc gtatttcacc tgaacagtgg tcctctcttt tgtatggtga tttggctcat aaatcacaca tgcagtctat cattgataag ctacagtctc cagagtcatt tgcaaagagt gtccaggaat tgacaattgt tttgcaacga acaggtgacc cagctaactt aaatagactg aggcctcatt tagagcttct tgcaaacata gaccctaatc cagacgctgt ttcaccaact tgggagcagc tggaaaatgc aatggtagct gttaaaacag tagttcatgg ccttgtggac ttcatacaaa attatagtag aaaaggccat gagacccctc agcctcagcc aaacagcaaa tacaagacta gcatgtgccg agatttgcga cagcaagggg gttgtccacg aggaacaaat tgtacatttg cccattctca ggaagagctt gaaaagtgta acccccgtgg actatatctt cattgttgtc tcctttcctc agtggctagc ttgggcactg tacataatga gctctacaag cagcagcatt gtgatagaga gaagataagt gtgtgtgcag tgaaaagagc agaaacatgc ttctcttctc agtttttttc cccacttgaa gagcctagag aagaggatta Protein function: Post-transcriptional repressor of mRNAs containing a conserved stem loop motif, called constitutive decay element (CDE), which is often located in the 3'-UTR, as in HMGXB3, ICOS, IER3, NFKBID, NFKBIZ, PPP1R10, TNF and in many more mRNAs. Binds to CDE and promotes mRNA deadenylation and degradation. This process does not involve miRNAs. In follicular helper T (Tfh) cells, represses of ICOS and TNFRSF4 expression, thus preventing spontaneous Tfh cell differentiation, germinal center B-cell differentiation in the absence of immunization and autoimmunity. In resting or LPS-stimulated macrophages, controls inflammation by suppressing TNF expression. Also recognizes CDE in its own mRNA and in that of paralogous RC3H1, possibly leading to feedback loop regulation. miRNA- binding protein that regulates microRNA homeostasis. Enhances DICER- mediated processing of pre-MIR146a but reduces mature MIR146a levels through an increase of 3' end uridylation. Both inhibits ICOS mRNA expression and they may act together to exert the suppression (PubMed:25697406). Acts as a ubiquitin E3 ligase. Pairs with E2 enzymes UBE2B, UBE2D2, UBE2E2, UBE2E3, UBE2G2, UBE2K and UBE2Q2 and produces polyubiquitin chains (PubMed:26489670). Shows the strongest activity when paired with UBE2N:UBE2V1 or UBE2N:UBE2V2 E2 complexes and generate both short and long polyubiquitin chains (PubMed:26489670). Involved in the ubiquitination of MAP3K5 (PubMed:24448648, PubMed:26489670). Able to interact with double-stranded RNA (dsRNA) (PubMed:26489670). [The UniProt Consortium]