QTRT1 (Vector Vector will be determined during the manufacturing process, either pENTR223.1 or pUC,

Length: 1077 Sequence: atgccagtgg gcacgcaggc caccatgaag ggcatcacga ccgaacagct ggacgctctg ggttgccgca tctgcctggg caatacctac catctgggtc taaggccggg acccgagctg atccagaaag ccaacggtct ccacggcttc atgaattggc ctcataatct gctaacggac agcggcggtt tccagatggt gtcgctggtg tctctgtccg aggtgacgga ggagggcgtc cgcttccgct ccccctacga cggcaatgag accctgctga gcccggagaa atccgtgcag atccagaatg cgctgggctc ggacatcatc atgcagctgg acgacgtggt tagcagtact gtgactgggc cacgtgtgga ggaggccatg tacaggtcaa tccgctggct ggaccggtgc attgcagccc atcagcggcc ggacaagcag aacctcttcg ccattatcca gggtgggctg gacgcagatc tccgggccac ctgccttgaa gagatgacca agcgagacgt gcctggcttc gccatcgggg gcctgagcgg gggtgagagc aagtcgcagt tctggcggat ggtggcgctg agcacctctc ggctgccgaa ggacaagccc cgatatctga tgggggttgg ctatgccact gatctggtag tctgcgtggc tcttggatgt gacatgttcg actgcgtctt ccccacacgg acagcgcgct ttggctctgc cctggtgccc actgggaacc tgcagttgag gaagaaggtg tttgagaagg acttcggccc catagacccg gagtgcacct gccccacgtg ccaaaagcac agccgcgcct tcctgcacgc actgctgcac agtgacaaca cggccgcgct gcaccacctc acggtccaca acatcgccta ccagctgcag ctcatgagcg ccgtccgcac cagcatcgtg gagaagcgct tcccggactt cgtgcgggac ttcatgggcg ccatgtacgg ggatcccacc ctctgtccca cctgggccac tgacgctctg gcctctgtgg gaatcacact Protein function: Catalytic subunit of the queuine tRNA-ribosyltransferase (TGT) that catalyzes the base-exchange of a guanine (G) residue with queuine (Q) at position 34 (anticodon wobble position) in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr), resulting in the hypermodified nucleoside queuosine (7-(((4,5-cis-dihydroxy-2- cyclopenten-1-yl)amino)methyl)-7-deazaguanosine) (PubMed:11255023, PubMed:20354154). Catalysis occurs through a double-displacement mechanism. The nucleophile active site attacks the C1' of nucleotide 34 to detach the guanine base from the RNA, forming a covalent enzyme-RNA intermediate. The proton acceptor active site deprotonates the incoming queuine, allowing a nucleophilic attack on the C1' of the ribose to form the product. [The UniProt Consortium]