PIWIL2 (Vector Vector will be determined during the manufacturing process, either pENTR223.1 or pUC,

Length: 2922 Sequence: atggatcctt tccgaccatc gttcaggggc cagtctccta tccacccatc ccagtgccag gctgtacgga tgccaggctg ttggccacaa gcttctaaac ctttggaccc agctctgggc aggggagcac ctgcaggcag aggccatgta tttggaaagc cagaggaacc aagcacacag agggggccag cacaaaggga gtctgtgggt ttggtctcca tgttccgagg cctgggcatt gaaacagttt ctaagacccc tctgaaacgg gaaatgcttc catcaggtag aggcatttta ggtcgaggct tgtctgctaa tctggtacgc aaggacaggg aggaactctc tcccactttt tgggatccaa aagtgttggc ggctggggac agcaagatgg cagagacctc cgttggttgg agtaggacgc ttggaagagg gagttcagat gcgtctttat taccactggg aagagcagca ggtggtatca gcagagaagt ggacaagcct ccctgtacct tcagcacacc gtcccggggt cccccgcagc tgtcatcacc accagctctg ccccagtctc ccctgcactc tccagatcgc cctctggtcc tgactgtgga acacaaggaa aaagagctta ttgtgaagca aggatcaaaa ggaacacctc agtctttggg actgaacctc gtcaaaatac agtgtcataa tgaagcagtt tatcaatatc atgtgacttt cagccccaat gtggagtgca aaagcatgag gttcggcatg ttgaaggacc atcaagctgt caccggcaac gtcactgcgt ttgatggatc tattctctat ctgcctgtta agcttcaaca agttcttgag ttaaaaagtc aaaggaaaac agacagtgct gaaatcagca ttaagattca gatgacaaag atcctggagc cctgctctga cctgtgcatt cccttctaca atgttgtttt ccgtcgggta atgaaacttt tagatatgaa gcttgtgggg agaaactttt atgaccctac aagtgctatg gtactacagc aacacagatt gcagatctgg ccaggctatg cagctagcat ccgaaggaca gatggagggc tcttcctgct agctgatgtc tcccataagg tcattcggaa tgactgtgtg ctggatgtca tgcatgccat ttatcagcag aataaagaac acttccagga tgagtgtact aagcttctgg ttggcaatat tgttatcacc cgatataaca atcgtaccta tcgtattgat gatgtggatt ggaataagac tccaaaggat agcttcacga tgtctgatgg gaaagagatc acattcttgg aatactacag caaaaattat gggatcacag ttaaggaaga ggaccagcca ttgctgattc acaggcccag tgagagacag gataatcatg ggatgctgct aaaaggggaa atcctgctgc tgcctgagct ttcttttatg accggaatcc cagagaagat gaagaaggac ttcagagcca tgaaggattt ggctcagcaa atcaatctga gccccaagca acaccatagt gctttggaat gcttgctgca aagaattgca aagaacgagg cagccaccaa tgaactgatg cgttgggggc tccgtctgca aaaggatgta cataagattg aaggacgtgt tctgccaatg gaaagaatta acttaaaaaa tacttcgttt atcacatctc aggaactaaa ctgggttaag gaagtaacca gagacccttc catcttgact atccccatgc atttctgggc acttttttac ccaaagagag caatggacca ggctcgagaa ctggtcaaca tgttggagaa gatagccggc cccattggca tgcgtatgag cccaccggcc tgggttgaac taaaggatga ccgaatagag acttatgtca gaaccattca atccacgtta ggagctgagg ggaagataca gatggttgtt tgcatcatca tgggcccacg tgatgatctc tatggggcca tcaagaagct gtgctgtgtg cagtccccag tgccctccca ggttgtcaat gttcgaacca ttggtcagcc caccaggctt cggagtgtgg cccagaagat tttacttcag attaactgta aattgggtgg tgagctctgg ggagtggata ttcctctgaa acagttaatg gtgatcggga tggatgttta ccatgacccc agtagaggca tgcgctccgt ggttggcttc gtggcaagca tcaatctcac cctcacaaaa tggtattccc gggtggtgtt ccagatgccg catcaggaga ttgtggacag cctgaagcta tgcctcgtgg gctccttaaa aaagttttat gaggtgaacc actgtctacc agagaagatt gtggtgtacc gtgatggagt gtctgatggc caactgaaga cagttgccaa ctatgagatt cctcaactac agaagtgttt tgaagctttt gagaattatc agcccaagat ggtggtgttt gtagttcaga agaaaatcag tactaatcta tatctggctg ctcctcagaa ctttgtaact cccactcctg gaactgtggt agatcataca ataacaagct gtgagtgggt ggatttctat cttcttgccc atcatgtacg gcagggctgt ggcattccta cgcattatgt ctgtgttctc aacaccgcaa acctgagccc tgatcatatg cagaggctga ctttcaaact gtgccacatg tactggaatt ggcctggcac catcagagtt ccagctcctt gcaagtatgc ccacaagcta gctttcctgt caggacacat cttgcatcat gaaccagcca tccagctgtg cgagaacctg ttcttcctgt Protein function: Endoribonuclease that plays a central role during spermatogenesis by repressing transposable elements and preventing their mobilization, which is essential for the germline integrity. Plays an essential role in meiotic differentiation of spermatocytes, germ cell differentiation and in self-renewal of spermatogonial stem cells. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and govern the methylation and subsequent repression of transposons. During piRNA biosynthesis, plays a key role in the piRNA amplification loop, also named ping-pong amplification cycle, by acting as a 'slicer-competent' piRNA endoribonuclease that cleaves primary piRNAs, which are then loaded onto 'slicer-incompetent' PIWIL4. PIWIL2 slicing produces a pre-miRNA intermediate, which is then processed in mature piRNAs, and as well as a 16 nucleotide by- product that is degraded. Required for PIWIL4/MIWI2 nuclear localization and association with secondary piRNAs antisense. Besides their function in transposable elements repression, piRNAs are probably involved in other processes during meiosis such as translation regulation. Indirectly modulates expression of genes such as PDGFRB, SLC2A1, ITGA6, GJA7, THY1, CD9 and STRA8. When overexpressed, acts as an oncogene by inhibition of apoptosis and promotion of proliferation in tumors (PubMed:16377660). Represses circadian rhythms by promoting the stability and activity of core clock components ARNTL/BMAL1 and CLOCK by inhibiting GSK3B-mediated phosphorylation and ubiquitination- dependent degradation of these proteins (PubMed:28903391). [The UniProt Consortium]