PIP5K1A (Vector Vector will be determined during the manufacturing process, either pENTR223.1 or pUC

Length: 1503 Sequence: atggcgtcgg cctcctccgg gccgtcgtct tcggtcggtt tttcatcctt tgatcccgcg gtcccttcct gtaccttgtc ctcagcatct ggaatcaaga gacccatggc atctgaggtg ccttatgcct ctggcatgcc catcaagaaa ataggccata gaagtgttga ttcctcagga gagacaacat ataaaaagac aacctcatca gccttgaaag gtgccatcca gttaggcatt acccacactg tggggagcct gagtaccaaa ccagagcgtg atgtcctcat gcaagatttc tacgtggttg agagtatctt ctttcccagt gaagggagca acctgacccc tgctcatcac tacaatgact ttcgtttcaa gacctatgca cctgttgcct tccgctactt ccgggagcta tttggtatcc ggcccgatga ttacttgtat tccctctgca gtgagccgct gattgaactc tgtagctctg gagctagtgg ttccctattc tatgtgtcca gcgacgatga gttcattatt aagacagtcc aacataaaga ggcggaattt ctgcagaagc tgcttccagg atactacatg aacctcaacc agaaccctcg gactttgctg cctaaattct atggactgta ctgtgtgcag gcaggtggca agaacattcg gattgtggtg atgaacaatc ttttaccaag atcggtaaaa atgcatatca aatatgacct caaaggctca acctacaaac ggcgggcttc ccagaaagag cgagagaagc ctcttcccac atttaaagac ctagacttct tacaagacat ccctgatggt ctttttttgg atgctgacat gtacaacgct ctctgtaaga ccctgcagcg tgactgtttg gtgctgcaga gcttcaagat aatggattat agcctcttga tgtcaatcca taatatagat catgcacaac gagagccctt aagcagtgaa acacagtact cagttgatac tcgaagaccg gccccccaaa aggctctgta ttccacagcc atggaatcca tccagggaga ggctcgacgg ggtggtacca tggagactga tgaccatatg ggtggcatcc ctgcccggaa tagtaaaggg gaaaggcttc tgctttatat tggcatcatt gacattctac agtcttacag gtttgttaag aagttggagc actcttggaa agccctggta catgacggag acactgtctc agtgcatcgc ccaggcttct acgctgaacg gttccagcgc ttcatgtgca acacagtatt taagaagatt ccctgcgttc accttggtcg tcctgatgtt ttacctcaga ctccaccttt ggaggaaatc agtgagggct cgcctattcc tgaccccagt ttctcacctc tagttggaga gactttgcaa atgctaacta caagtacaac cttggaaaag cttgaagttg cagagtcaga gttcacccat Protein function: Catalyzes the phosphorylation of phosphatidylinositol 4- phosphate (PtdIns(4)P/PI4P) to form phosphatidylinositol 4,5- bisphosphate (PtdIns(4,5)P2/PIP2), a lipid second messenger that regulates several cellular processes such as signal transduction, vesicle trafficking, actin cytoskeleton dynamics, cell adhesion, and cell motility (PubMed:8955136, PubMed:21477596, PubMed:22942276). PtdIns(4,5)P2 can directly act as a second messenger or can be utilized as a precursor to generate other second messengers: inositol 1,4,5- trisphosphate (IP3), diacylglycerol (DAG) or phosphatidylinositol- 3,4,5-trisphosphate (PtdIns(3,4,5)P3/PIP3) (PubMed:19158393, PubMed:20660631). PIP5K1A-mediated phosphorylation of PtdIns(4)P is the predominant pathway for PtdIns(4,5)P2 synthesis. Can also use phosphatidylinositol (PtdIns) as substrate in vitro (PubMed:22942276). Together with PIP5K1C, is required for phagocytosis, both enzymes regulating different types of actin remodeling at sequential steps. Promotes particle ingestion by activating the WAS GTPase-binding protein that induces Arp2/3 dependent actin polymerization at the nascent phagocytic cup. Together with PIP5K1B, is required, after stimulation by G-protein coupled receptors, for the synthesis of IP3 that will induce stable platelet adhesion. Recruited to the plasma membrane by the E-cadherin/beta-catenin complex where it provides the substrate PtdIns(4,5)P2 for the production of PtdIns(3,4,5)P3, IP3 and DAG, that will mobilize internal calcium and drive keratinocyte differentiation (PubMed:19158393). Positively regulates insulin-induced translocation of SLC2A4 to the cell membrane in adipocytes. Together with PIP5K1C has a role during embryogenesis. Independently of its catalytic activity, is required for membrane ruffling formation, actin organization and focal adhesion formation during directional cell migration by controlling integrin-induced translocation of the small GTPase RAC1 to the plasma membrane (PubMed:20660631). Also functions in the nucleus where it acts as an activator of TUT1 adenylyltransferase activity in nuclear speckles, thereby regulating mRNA polyadenylation of a select set of mRNAs (PubMed:18288197). [The UniProt Consortium]