PAN3 (Vector pUC, Accession No. BC128180)

Length: 2064 Sequence: ATGGATGGAG GTGCTTTAAC TGATACAAGT CTCACAGATT CCTATTTTAG CACCAGCTTT ATTGGAGTCA ATGGATTTGG AAGCCCTGTA GAAACAAAAT ATCCCCTGAT GCAGAGAATG ACTAATAGTA GCAGCTCCCC AAGCCTTCTA AATGACAGTG CCAAGCCATA TTCAGCCCAT GATCCTCTAA CATCACCTGC TTCATCCTTG TTTAATGACT TTGGTGCCCT CAACATCTCT CAGAGACGAA AGACACCAAA TCCTACTGCA AGCGAGTTTA TTCCTAAAGG AGGATCAACC TCCAGGCTGA GTAACGTGTC CCAGTCAAAT ATGTCTGCCT TCTCTCAAGT TTTCTCTCAC CCATCCATGG GAAGCCCTGC TACTGCTGGA TTAGCGCCAG GAATGTCGTT GTCTGCTGGG TCTTCCCCTC TTCATTCCCC CAAGATTACT CCACATACTT CTCCTGCTCC CAGAAGAAGA AGTCACACTC CAAATCCAGC AAGTTACATG GTGCCTTCTA GTGCCTCTAC ATCTGTTAAT AATCCTGTTT CTCAGACTCC GTCTTCTGGT CAGGTGATCC AAAAGGAAAC TGTTGGTGGG ACGACTTACT TCTATACAGA CACAACTCCA GCACCTTTGA CTGGAATGGT GTTTCCAAAC TATCATATTT ATCCTCCAAC TGCACCTCAC GTTGCTTATA TGCAACCGAA AGCAAACGCA CCTTCCTTCT TCATGGCTGA TGAACTCCGA CAGGAGCTGA TCAACAGACA TTTAATAACA ATGGCTCAAA TTGATCAAGC AGATATGCCA GCAGTTCCTA CAGAGGTTGA CAGCTACCAT AGCCTATTCC CTCTAGAACC ACTGCCACCT CCCAACCGGA TACAGAAATC AAGTAATTTT GGATATATTA CATCTTGCTA CAAAGCTGTA AACAGCAAAG ATGATCTGCC ATATTGCCTT CGGAGGATAC ATGGTTTTCG TCTTGTTAAC ACAAAGTGCA TGGTGTTGGT CGACATGTGG AAGAAAATTC AACACTCAAA TATCGTAACT TTGCGTGAAG TATTTACCAC TAAAGCATTT GCTGAGCCCT CTCTTGTGTT TGCACATGAT TTCCATGCTG GAGGAGAAAC TATGATGAGC AGACACTTTA ATGACCCTAA TGCTGATGCC TACTTCACCA AGAGAAAGTG GGGTCAGCAC GAGGGACCAT TGCCCAGGCA GCATGCTGGA TTATTGCCAG AATCTCTTAT TTGGGCATAT ATTGTCCAAC TAAGTTCTGC ATTGCGTACC ATTCATACAG CAGGTTTGGC ATGTCGAGTT ATGGATCCAA CAAAGATTCT GATAACTGGC AAAACAAGGT TGCGAGTAAA TTGTGTTGGA GTTTTTGATG TTTTAACATT TGATAACAGT CAAAATAATA ATCCATTGGC ATTAATGGCC CAGTACCAGC AAGCAGATCT GATATCATTA GGAAAAGTTG TGTTGGCTTT GGCTTGCAAC TCTTTGGCAG GAATTCAGCG AGAGAATTTA CAGAAAGCCA TGGAACTGGT GACAATCAAC TATTCCTCTG ACCTGAAGAA TCTGATTTTG TATTTGTTGA CTGACCAAAA CAGGATGCGA AGTGTAAATG ACATCATGCC CATGATTGGT GCTCGATTTT ATACTCAATT GGATGCTGCT CAAATGAGAA ATGATGTCAT AGAGGAAGAC CTTGCAAAGG AGGTTCAAAA TGGAAGACTG TTTAGGCTCC TAGCAAAATT GGGAACAATC AATGAGAGGC CGGAGTTTCA GAAGGATCCC ACTTGGTCAG AGACTGGAGA CCGTTATCTG TTGAAACTCT TTAGGGATCA TCTTTTTCAT CAGGTGACAG AAGCAGGTGC TCCCTGGATT GACCTCAGTC ATATAATTTC TTGTCTTAAC AAGCTAGATG CTGGTGTGCC AGAAAAAATA AGCCTGATTT CCAGAGATGA GAAGAGTGTA CTTGTGGTGA CCTACAGTGA CTTAAAGCGC TGCTTTGAAA ATACTTTTCA AGAACTGATT GCAGCTGCAA ATGGTCAGTT Protein function: Regulatory subunit of the poly(A)-nuclease (PAN) deadenylation complex, one of two cytoplasmic mRNA deadenylases involved in general and miRNA-mediated mRNA turnover. PAN specifically shortens poly(A) tails of RNA and the activity is stimulated by poly(A)-binding protein (PABP). PAN deadenylation is followed by rapid degradation of the shortened mRNA tails by the CCR4-NOT complex. Deadenylated mRNAs are then degraded by two alternative mechanisms, namely exosome-mediated 3'-5' exonucleolytic degradation, or deadenlyation-dependent mRNA decaping and subsequent 5'-3' exonucleolytic degradation by XRN1. PAN3 acts as a positive regulator for PAN activity, recruiting the catalytic subunit PAN2 to mRNA via its interaction with RNA and PABP, and to miRNA targets via its interaction with GW182 family proteins. [The UniProt Consortium]