NEK2 (Vector Vector will be determined during the manufacturing process, either pENTR223.1 or pUC, A

Length: 645 Sequence: atgccttccc gggctgagga ctatgaagtg ttgtacacca ttggcacagg ctcctacggc cgctgccaga agatccggag gaagagtgat ggcaagatat tagtttggaa agaacttgac tatggctcca tgacagaagc tgagaaacag atgcttgttt ctgaagtgaa tttgcttcgt gaactgaaac atccaaacat cgttcgttac tatgatcgga ttattgaccg gaccaataca acactgtaca ttgtaatgga atattgtgaa ggaggggatc tggctagtgt aattacaaag ggaaccaagg aaaggcaata cttagatgaa gagtttgttc ttcgagtgat gactcagttg actctggccc tgaaggaatg ccacagacga agtgatggtg gtcataccgt attgcatcgg gatctgaaac cagccaatgt tttcctggat ggcaagcaaa acgtcaagct tggagacttt gggctagcta gaatattaaa ccacgacacg agttttgcaa aaacatttgt tggcacacct tattacatgt ctcctgaaca aatgaatcgc atgtcctaca atgagaaatc agatatctgg tcattgggct gcttgctgta tgagttatgt gcattaatct Protein function: Protein kinase which is involved in the control of centrosome separation and bipolar spindle formation in mitotic cells and chromatin condensation in meiotic cells. Regulates centrosome separation (essential for the formation of bipolar spindles and high-fidelity chromosome separation) by phosphorylating centrosomal proteins such as CROCC, CEP250 and NINL, resulting in their displacement from the centrosomes. Regulates kinetochore microtubule attachment stability in mitosis via phosphorylation of NDC80. Involved in regulation of mitotic checkpoint protein complex via phosphorylation of CDC20 and MAD2L1. Plays an active role in chromatin condensation during the first meiotic division through phosphorylation of HMGA2. Phosphorylates: PPP1CC, SGO1, NECAB3 and NPM1. Essential for localization of MAD2L1 to kinetochore and MAPK1 and NPM1 to the centrosome. Phosphorylates CEP68 and CNTLN directly or indirectly (PubMed:24554434). NEK2-mediated phosphorylation of CEP68 promotes CEP68 dissociation from the centrosome and its degradation at the onset of mitosis (PubMed:25704143). Involved in the regulation of centrosome disjunction (PubMed:26220856). [The UniProt Consortium]