MOCS3 (Vector Vector will be determined during the manufacturing process, either pENTR223.1 or pUC,

Length: 1383 Sequence: atggcttccc gggaggaggt actcgcctta caagctgaag ttgcccaacg tgaggaggaa ttgaattcgc tgaagcagaa gctggcgtcg gctcttttgg ctgagcagga accgcagcca gaacggctgg ttccggtgtc gccgctgccg ccgaaggccg ctctgtcccg agatgagatt ctgcgctata gccggcagct agtgctgccc gagctgggcg tgcacggaca gctgcgcctg gggaccgcgt gcgtgctaat cgtgggctgc ggtggactcg gctgtccact agcgcagtac ttggcagcgg ccggcgtggg ccgccttggc cttgtggact atgacgtggt agagatgagc aacctggccc gccaagtgct gcatggcgag gcactggctg gccaggccaa ggccttttcg gccgccgcct cgctgcgccg cctcaattcg gcagtggaat gcgtgccgta cactcaggcc cttacgccag ccactgccct agacctggtc cgccgatatg atgtggtggc tgactgctcg gacaacgtgc ccactcgcta cctggttaat gacgcatgtg tgctggcggg tcggcccctc gtgtctgcca gtgccttgcg cttcgagggc caaatcacag tctaccatta tgacggtggc ccttgctatc gctgcatatt cccccaacca cccccagcgg agacagtgac caactgcgcg gacggcgggg tgctcggtgt cgttaccggg gtcctgggct gcctgcaggc cttggaagtg ctgaaaatcg ctgcgggtct gggcccctct tacagtggca gcttgttgct ctttgatgcc ctgagagggc atttccgctc tattcggctg cggagccgca ggctcgactg tgcagcttgc ggggaacggc ccactgtgac tgatctgctg gactatgaag ccttctgtgg ctcctcagcc actgataaat gccgctccct gcaactactg agcccagagg agcgtgtttc tgtcaccgac tataagcgac tgctggattc tggggcattc cacctgttgc tggacgtcag gcctcaggtg gaggtggaca tttgtcgttt gcctcatgcc ctacacatcc ctctgaaaca tttggaacgc agggatgcgg agagcctgaa actcttaaaa gaagcaatct gggaagagaa gcagggcaca caagaagggg ctgctgtccc catttatgtg atttgcaaac tgggaaatga ctcacagaaa gccgtgaaga tcctccagtc cttatcagca gctcaagagt tagacccttt aacagttcgg gatgttgtgg ggggcctcat ggcctgggct gccaaaatcg atggaacatt tccacagtac Protein function: Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of cytosolic tRNA(Lys), tRNA(Glu) and tRNA(Gln). Also essential during biosynthesis of the molybdenum cofactor. Acts by mediating the C-terminal thiocarboxylation of sulfur carriers URM1 and MOCS2A. Its N-terminus first activates URM1 and MOCS2A as acyl- adenylates (-COAMP), then the persulfide sulfur on the catalytic cysteine is transferred to URM1 and MOCS2A to form thiocarboxylation (- COSH) of their C-terminus. The reaction probably involves hydrogen sulfide that is generated from the persulfide intermediate and that acts as nucleophile towards URM1 and MOCS2A. Subsequently, a transient disulfide bond is formed. Does not use thiosulfate as sulfur donor, NFS1 probably acting as a sulfur donor for thiocarboxylation reactions. [The UniProt Consortium]