MCOLN1 (Vector Vector will be determined during the manufacturing process, either pENTR223.1 or pUC,

Length: 1743 Sequence: atgacagccc cggcgggtcc gcgcggctca gagaccgagc ggcttctgac ccccaacccc gggtatggga cccaggcggg gccttcaccg gcccctccga cacccccaga agaggaagac cttcgccgtc gtctcaaata ctttttcatg agtccctgcg acaagtttcg agccaagggc cgcaagccct gcaagctgat gctgcaagtg gtcaagatcc tggtggtcac ggtgcagctc atcctgtttg ggctcagtaa tcagctggct gtgacattcc gggaagagaa caccatcgcc ttccgacacc tcttcctgct gggctactcg gacggagcgg atgacacctt cgcagcctac acgcgggagc agctgtacca ggccatcttc catgctgtgg accagtacct ggcgttgcct gacgtgtcac tgggccggta tgcgtatgtc cgtggtgggg gtgacccttg gaccaatggc tcagggcttg ctctctgcca gcggtactac caccgaggcc acgtggaccc ggccaacgac acatttgaca ttgatccgat ggtggttact gactgcatcc aggtggatcc ccccgagcgg ccccctccgc cccccagcga cgatctcacc ctcttggaaa gcagctccag ttacaagaac ctcacgctca aattccacaa gctggtcaat gtcaccatcc acttccggct gaagaccatt aacctccaga gcctcatcaa taatgagatc ccggactgct ataccttcag cgtcctgatc acgtttgaca acaaagcaca cagtgggcgg atccccatca gcctggagac ccaggcccac atccaggagt gtaagcaccc cagtgtcttc cagcacggag acaacagctt ccggctcctg tttgacgtgg tggtcatcct cacctgctcc ctgtccttcc tcctctgcgc ccgctcactc cttcgaggct tcctgctgca gaacgagttt gtggggttca tgtggcggca gcggggacgg gtcatcagcc tgtgggagcg gctggaattt gtcaatggct ggtacatcct gctcgtcacc agcgatgtgc tcaccatctc gggcaccatc atgaagatcg gcatcgaggc caagaacttg gcgagctacg acgtctgcag catcctcctg ggcacctcga cgctgctggt gtgggtgggc gtgatccgct acctgacctt cttccacaac tacaatatcc tcatcgccac actgcgggtg gccctgccca gcgtcatgcg cttctgctgc tgcgtggctg tcatctacct gggctactgc ttctgtggct ggatcgtgct ggggccctat catgtgaagt tccgctcact ctccatggtg tctgagtgcc tgttctcgct catcaatggg gacgacatgt ttgtgacgtt cgccgccatg caggcgcagc agggccgcag cagcctggtg tggctcttct cccagctcta cctttactcc ttcatcagcc tcttcatcta catggtgctc agcctcttca tcgcgctcat caccggcgcc tacgacacca tcaagcatcc cggcggcgca ggcgcagagg agagcgagct gcaggcctac atcgcacagt gccaggacag ccccacctcc ggcaagttcc gccgcgggag cggctcggcc tgcagccttc tctgctgctg cggaagggac ccctcggagg agcattcgct gctggtgaat Protein function: Nonselective cation channel probably playing a role in the regulation of membrane trafficking events and of metal homeostasis. Proposed to play a major role in Ca(2+) release from late endosome and lysosome vesicles to the cytoplasm, which is important for many lysosome-dependent cellular events, including the fusion and trafficking of these organelles, exocytosis and autophagy (PubMed:11013137, PubMed:12459486, PubMed:15336987, PubMed:14749347, PubMed:29019983, PubMed:27623384). Required for efficient uptake of large particles in macrophages in which Ca(2+) release from the lysosomes triggers lysosomal exocytosis. May also play a role in phagosome-lysosome fusion. Involved in lactosylceramide trafficking indicative for a role in the regulation of late endocytic membrane fusion/fission events (PubMed:16978393). By mediating lysosomal Ca(2+) release is involved in regulation of mTORC1 signaling and in mTOR/TFEB-dependent lysosomal adaptation to environmental cues such as nutrient levels (PubMed:27787197, PubMed:25733853). Seems to act as lysosomal active oxygen species (ROS) sensor involved in ROS- induced TFEB activation and autophagy (PubMed:27357649). Functions as a Fe(2+) permeable channel in late endosomes and lysosomes (PubMed:18794901). Proposed to play a role in zinc homeostasis probably implicating its association with TMEM163 (PubMed:25130899) In adaptive immunity, TRPML2 and TRPML1 may play redundant roles in the function of the specialized lysosomes of B cells. [The UniProt Consortium]