MAGEF1 (Vector Vector will be determined during the manufacturing process, either pENTR223.1 or pUC,

Length: 927 Sequence: atgttgcaga caccagagag cagggggctc ccggtcccgc aggccgaggg ggagaaggat ggcggccatg atggtgagac ccgggccccg accgcctcgc aggagcgccc caaggaggag cttggcgccg ggagggagga gggggctgcg gagcccgccc tcacccggaa aggcgcgagg gccttggcgg ccaaatcctt ggcaaggcgc agggcctacc gccggctgaa tcggacggtg gcggagttgg tgcagttcct cctggtgaaa gacaagaaga agagtcccat cacacgctcg gagatggtga aatacgttat tggagacttg aagattctgt tcccggacat catcgcaagg gccgcagagc atctgcggta tgtctttggt tttgagctga aacagtttga ccgcaagcac cacacttaca tcctgatcaa caaactaaaa cctctggagg aggaggagga ggaggaggat ctgggaggag atggccccag attgggtctg ttaatgatga tcctgggcct tatctatatg agaggtaata gcgccaggga ggcccaggtc tgggagatgc tgcgtcggtt gggggtgcaa ccctcaaagt atcatttcct ctttgggtat ccgaagaggc ttattatgga agattttgtg cagcagcgat atctcagtta caggcgggtg cctcacacca atccaccagc atatgaattc tcttggggtc cccgaagcaa cctggaaatc agcaagatgg aagtcctggg gttcgtggcc aaactgcata agaaggaacc gcagcactgg ccagtgcagt accgtgaggc cctagcagac gaggccgaca gggccagagc caaggccaga gctgaagcca gtatgagggc cagggccagt gctagggccg gcatccacct Protein function: Enhances ubiquitin ligase activity of RING-type zinc finger- containing E3 ubiquitin ligases. Proposed to act through recruitment and/or stabilization of the E2 ubiquitin-conjugating enzyme at the E3:substrate complex. MAGEF1-NSMCE1 ubiquitin ligase complex promotes proteasomal degradation of MMS19, a key component of the cytosolic iron-sulfur protein assembly (CIA) machinery. Down-regulation of MMS19 impairs the activity of several DNA repair and metabolism enzymes such as ERCC2/XPD, FANCJ, RTEL1 and POLD1 that require iron-sulfur clusters as cofactors. May negatively regulate genome integrity by inhibiting homologous recombination-mediated double-strand break DNA repair (PubMed:29225034). [The UniProt Consortium]