LARP1 (Vector pUC, Accession No. BC001460)

Length: 3060 Sequence: atgctttgga gggtgctttt gtcaaagagg cctcctttcc ctcacccaga gctggatttc caagaggctc ccatacctag ctgccctggc agactcccag ggaggaaaaa cagcgtggcc ttggcagctg ccccgaggaa ggagcccaca ggtgacaggg agaagccatt gccattccct gtcctggccc ccttcagcaa ccctgaacac tctgctccag ccaaggtggt gagggcagct gttcctaaac agcgcaaagg cagcaaggtt ggtgactttg gagatgcaat caattggccc acacctggag agatagccca caagagtgtt cagccacagt cccacaagcc tcagcctacc cgtaaactgc cacccaagaa ggacatgaag gaacaggaga aaggagaagg gagtgatagt aaggagagtc caaaaaccaa atcagatgaa tcaggggagg aaaagaatgg agatgaggat tgccagcgag gcgggcagaa gaagaaagga aacaaacaca agtgggttcc attacaaata gacatgaagc ctgaagtgcc cagagagaaa ctggcttcac gccccactcg cccaccggag cctagacaca tacctgccaa tcgcggagag atcaaagggt ctgagtctgc cacctacgtg cccgtggccc cccccacccc agcctggcaa ccagagatca aaccggagcc tgcctggcac gaccaggatg agacatcgag tgtgaagagt gatggggctg gtggggcgcg ggcttccttc cgtggccgtg gacgggggcg tggtcgcggc cggggacgcg gccggggtgg cactcgaacc cattttgact accagtttgg ctaccgaaag tttgatggtg tggaggggcc tcgtacgccc aagtacatga acaacatcac ctactacttt gacaatgtca gcagcaccga gctttacagt gtggatcagg aactgctcaa agactacatc aagcgccaga ttgaatacta cttcagcgtg gacaatttag agcgagactt cttcctgcga aggaaaatgg atgctgatgg tttcctaccc atcaccctta ttgcttcctt ccaccgagtg caggccctta ccactgacat ttcactcatc tttgcggccc taaaggacag caaggtggtg gagatcgttg atgagaaagt tcgtaggagg gaggaaccag aaaagtggcc tcttccccca atagtggatt attcacagac tgatttctcc cagcttctca actgccctga atttgttccc cgtcagcact accaaaagga gacagagtcg gcacctggct ctcctcgtgc agtcacccca gtgccaacca aaacagagga ggtcagcaac ctaaagacac tacccaaggg cctgtctgcc agcctgcctg acctggattc tgagaactgg attgaagtga agaagaggcc tcggccatcc ccagcacggc ccaagaagtc agaggagtcc agattttccc acctgacctc cctgcctcag cagctgcctt cccagcagct gatgtccaag gatcaggatg agcaagagga actggatttt ctgtttgacg aggagatgga gcagatggat gggcggaaga acaccttcac tgcctggtct gatgaggaat ctgactatga gattgatgac agggatgtca acaagatcct cattgtcacc cagacaccac attacatgcg ccggcaccca gggggggacc gcacaggcaa ccacacctcg cgtgccaaga tgagcgccga actggccaag gtcattaatg atggcctctt ctactatgag caggacctgt gggctgaaaa gtttgaacct gagtattccc agatcaagca agaagtcgag aacttcaaaa aggtcaatat gatcagccgg gagcagtttg acacactgac ccctgagccc cctgtggatc ccaaccagga agttcctcct gggccacctc ggttccagca agttcctacg gatgccctgg ccaacaagtt gtttggtgct cctgagccct ccaccatcgc ccgctctcta ccaaccactg tcccagagtc accaaactac cgcaacacca ggacccctcg cactccccgg acaccacagc tcaaagactc aagccagaca tcacggtttt acccagtggt gaaagaagga cggacactgg atgccaagat gcctcgaaaa agaaagacaa gacacagttc aaacccaccc ttggagagcc atgtgggctg ggtgatggat tcccgtgagc acaggccccg tactgcttcc atcagctcca gcccctcaga agggacgcct acagttggca gctatggctg tacccctcag tcattgccca agttccagca tccttcccat gaactgctca aggaaaatgg cttcacacaa cacgtctacc ataagtatcg taggcgctgc cttaatgagc ggaaacgctt gggcattggc cagtctcagg agatgaacac actcttccgc ttctggtcct tcttcctccg agatcacttc aacaaaaaga tgtatgagga gttcaagcag ctggctctgg aggacgccaa agaaggctac agatatggtt tggagtgcct ttttcgatac tacagttatg gcctggaaaa gaagttccgg ctggacatat tcaaggattt tcaggaggaa acggtgaagg actatgaagc tggccaactg tatgggctgg agaagttctg ggccttcttg aaatattcca aagccaaaaa tttggacatt gaccccaaac tgcaagaata cctcggcaaa ttccgacgtc ttgaagactt ccgagtagat ccccccatgg gtgaggaggg caaccacaag cgacactcag tggtagcagg aggtggcggc ggtgagggca ggaagcggtg cccctcccag tcttccagca ggcctgctgc catgatcagc caacccccta caccacccac cggccagcct gtccgggaag atgccaaatg gacaagccag cactcgaaca cacagacttt Protein function: RNA-binding protein that regulates the translation of specific target mRNA species downstream of the mTORC1 complex, in function of growth signals and nutrient availability (PubMed:20430826, PubMed:23711370, PubMed:24532714, PubMed:25940091, PubMed:28650797, PubMed:28673543, PubMed:29244122). Interacts on the one hand with the 3' poly-A tails that are present in all mRNA molecules, and on the other hand with the 7-methylguanosine cap structure of mRNAs containing a 5' terminal oligopyrimidine (5'TOP) motif, which is present in mRNAs encoding ribosomal proteins and several components of the translation machinery (PubMed:23711370, PubMed:25940091, PubMed:28650797, PubMed:29244122, PubMed:26206669, PubMed:28379136). The interaction with the 5' end of mRNAs containing a 5'TOP motif leads to translational repression by preventing the binding of EIF4G1 (PubMed:25940091, PubMed:28650797, PubMed:29244122, PubMed:28379136). When mTORC1 is activated, LARP1 is phosphorylated and dissociates from the 5' untranslated region (UTR) of mRNA (PubMed:25940091, PubMed:28650797). Does not prevent binding of EIF4G1 to mRNAs that lack a 5'TOP motif (PubMed:28379136). Interacts with the free 40S ribosome subunit and with ribosomes, both monosomes and polysomes (PubMed:20430826, PubMed:24532714, PubMed:25940091, PubMed:28673543). Under normal nutrient availability, interacts primarily with the 3' untranslated region (UTR) of mRNAs encoding ribosomal proteins and increases protein synthesis (PubMed:23711370, PubMed:28650797). Associates with actively translating ribosomes and stimulates translation of mRNAs containing a 5'TOP motif, thereby regulating protein synthesis, and as a consequence, cell growth and proliferation (PubMed:20430826, PubMed:24532714). Stabilizes mRNAs species with a 5'TOP motif, which is required to prevent apoptosis (PubMed:20430826, PubMed:23711370, PubMed:25940091, PubMed:28673543). [The UniProt Consortium]