LAPTM4B (Vector Vector will be determined during the manufacturing process, either pENTR223.1 or pUC

Length: 681 Sequence: atgaagatgg tcgcgccctg gacgcggttc tactccaaca gctgctgctt gtgctgccat gtccgcaccg gcaccatcct gctcggcgtc tggtatctga tcatcaatgc tgtggtactg ttgattttat tgagtgccct ggctgatccg gatcagtata acttttcaag ttctgaactg ggaggtgact ttgagttcat ggatgatgcc aacatgtgca ttgccattgc gatttctctt ctcatgatcc tgatatgtgc tatggctact tacggagcgt acaagcaacg cgcagcctgg atcatcccat tcttctgtta ccagatcttt gactttgccc tgaacatgtt ggttgcaatc actgtgctta tttatccaaa ctccattcag gaatacatac ggcaactgcc tcctaatttt ccctacagag atgatgtcat gtcagtgaat cctacctgtt tggtccttat tattcttctg tttattagca ttatcttgac ttttaagggt tacttgatta gctgtgtttg gaactgctac cgatacatca atggtaggaa ctcctctgat gtcctggttt atgttaccag caatgacact acggtgctgc tacccccgta tgatgatgcc actgtgaatg gtgctgccaa ggagccaccg ccaccttacg tgtctgccta Protein function: Required for optimal lysosomal function (PubMed:21224396). Blocks EGF-stimulated EGFR intraluminal sorting and degradation. Conversely by binding with the phosphatidylinositol 4,5-bisphosphate, regulates its PIP5K1C interaction, inhibits HGS ubiquitination and relieves LAPTM4B inhibition of EGFR degradation (PubMed:25588945). Recruits SLC3A2 and SLC7A5 (the Leu transporter) to the lysosome, promoting entry of leucine and other essential amino acid (EAA) into the lysosome, stimulating activation of proton-transporting vacuolar (V)-ATPase protein pump (V-ATPase) and hence mTORC1 activation (PubMed:25998567). Plays a role as negative regulator of TGFB1 production in regulatory T cells (PubMed:26126825). Binds ceramide and facilitates its exit from late endosome in order to control cell death pathways (PubMed:26280656). [The UniProt Consortium]