KLHL22 (Vector Vector will be determined during the manufacturing process, either pENTR223.1 or pUC,

Length: 1905 Sequence: atggcagagg agcaggagtt cacccagctc tgcaagttgc ctgcacagcc ctcacaccca cactgcgtga acaacaccta ccgcagcgca cagcactccc aggctctgct ccgagggctg ctggctctcc gggacagcgg aatcctcttc gatgttgtgc tggtggtgga gggcagacac atcgaggccc atcgcatcct gctggctgcg tcctgcgatt acttcagagg aatgtttgct gggggattga aggagatgga acaggaagag gtcctgatcc acggtgtgtc ctacaatgct atgtgccaaa tcctacattt catatacacc tccgagctgg agctcagcct gagcaatgta caagagacac tggtggctgc ctgccagctt cagatcccag aaattatcca tttctgctgt gatttcctca tgtcctgggt ggacgaagag aacattctcg atgtctaccg gctggcagag ctgtttgact tgagccgcct gactgagcaa ctggacacct atatcctcaa aaactttgtg gccttctctc ggactgacaa gtaccgccag cttccattgg agaaggtcta ctccctcctc agcagcaatc gcctggaggt ctcctgcgag accgaggtat atgagggggc ccttctctac cattatagcc tggagcaggt gcaggctgac cagatctcgc tgcacgagcc cccaaagctc cttgagacag tgcggtttcc gctgatggaa gctgaggtcc tgcagcggct gcatgacaag ctggacccca gccctttgag ggacacagtg gccagcgccc tcatgtacca ccggaacgag agcctacagc ccagcctgca gagcccgcaa acggagctgc ggtcggactt ccagtgcgtt gtgggcttcg ggggcattca ctccacgccg tccactgtcc tcagcgacca ggccaagtat ctaaacccct tactgggaga gtggaagcac ttcactgcct ccctggcccc ccgcatgtcc aaccagggca tcgcggtgct caacaacttc gtatacttga ttggagggga caacaatgtc caaggatttc gagcagagtc ccgatgctgg aggtatgacc cacggcacaa ccgctggttc cagatccagt ccctgcagca ggagcacgcc gacctgtccg tgtgtgttgt aggcaggtac atctacgctg tggcgggccg tgactaccac aatgacctga atgctgtgga gcgctacgac cctgccacca actcctgggc atacgtggcc ccactcaaga gggaggtgta tgcccacgca ggcgcgacgc tggaggggaa gatgtatatc acctgcggcc gcagagggga ggattacctg aaagagacac actgctacga tccaggcagc aacacttggc acacactggc tgatgggcct gtgcggcgcg cctggcacgg catggcaacc ctcctcaaca agctgtatgt gatcgggggc agcaacaacg atgccggata caggagggac gtgcaccagg tggcctgcta cagctgcacg tctggacagt ggtcatctgt ctgcccactc cctgctgggc acggtgagcc tggcattgct gtgctggaca acaggatcta tgtgttaggt ggccgctcac acaaccgcgg cagccgcaca ggctacgtgc acatttacga tgtggagaag gactgctggg aggaagggcc ccagctggac aactccatct caggcctggc ggcctgtgtg ctcaccctgc cccgctccct gctccttgag ccgccccgcg ggacccctga ccgcagccag gccgacccgg actttgcctc tgaggtgatg agtgtgtctg actgggagga gtttgacaac tccagtgagg Protein function: Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complex required for chromosome alignment and localization of PLK1 at kinetochores. The BCR(KLHL22) ubiquitin ligase complex mediates monoubiquitination of PLK1, leading to PLK1 dissociation from phosphoreceptor proteins and subsequent removal from kinetochores, allowing silencing of the spindle assembly checkpoint (SAC) and chromosome segregation. Monoubiquitination of PLK1 does not lead to PLK1 degradation (PubMed:19995937, PubMed:23455478). The BCR(KLHL22) ubiquitin ligase complex is also responsible for the amino acid-stimulated 'Lys-48' polyubiquitination and proteasomal degradation of DEPDC5. Through the degradation of DEPDC5, releases the GATOR1 complex-mediated inhibition of the TORC1 pathway. It is therefore an amino acid-dependent activator within the amino acid-sensing branch of the TORC1 pathway, indirectly regulating different cellular processes including cell growth and autophagy (PubMed:29769719). [The UniProt Consortium]