ITSN1 (Vector pUC, Accession No. BC116186)

Length: 5166 Sequence: ATGGCTCAGT TTCCAACACC TTTTGGTGGC AGCCTGGATA TCTGGGCCAT AACTGTAGAG GAAAGAGCGA AGCATGATCA GCAGTTCCAT AGTTTAAAGC CAATATCTGG ATTCATTACT GGTGATCAAG CTAGAAACTT TTTTTTTCAA TCTGGGTTAC CTCAACCTGT TTTAGCACAG ATATGGGCAC TAGCTGACAT GAATAATGAT GGAAGAATGG ATCAAGTGGA GTTTTCCATA GCTATGAAAC TTATCAAACT GAAGCTACAA GGATATCAGC TACCCTCTGC ACTTCCCCCT GTCATGAAAC AGCAACCAGT TGCTATTTCT AGCGCACCAG CATTTGGTAT GGGAGGTATC GCCAGCATGC CACCGCTTAC AGCTGTTGCT CCAGTGCCAA TGGGATCCAT TCCAGTTGTT GGAATGTCTC CAACCCTAGT ATCTTCTGTT CCCACAGCAG CTGTGCCCCC CCTGGCTAAC GGGGCTCCCC CTGTTATACA ACCTCTGCCT GCATTTGCTC ATCCTGCAGC CACATTGCCA AAGAGTTCTT CCTTTAGTAG ATCTGGTCCA GGGTCACAAC TAAACACTAA ATTACAAAAG GCACAGTCAT TTGATGTGGC CAGTGTCCCA CCAGTGGCAG AGTGGGCTGT TCCTCAGTCA TCAAGACTGA AATACAGGCA ATTATTCAAT AGTCATGACA AAACTATGAG TGGACACTTA ACAGGTCCCC AAGCAAGAAC TATTCTTATG CAGTCAAGTT TACCACAGGC TCAGCTGGCT TCAATATGGA ATCTTTCTGA CATTGATCAA GATGGAAAAC TTACAGCAGA GGAATTTATC CTGGCAATGC ACCTCATTGA TGTAGCTATG TCTGGCCAAC CACTGCCACC TGTCCTGCCT CCAGAATACA TTCCACCTTC TTTTAGAAGA GTTCGATCTG GCAGTGGTAT ATCTGTCATA AGCTCAACAT CTGTAGATCA GAGGCTACCA GAGGAACCAG TTTTAGAAGA TGAACAACAA CAATTAGAAA AGAAATTACC TGTAACGTTT GAAGATAAGA AGCGGGAGAA CTTTGAACGT GGCAACCTGG AACTGGAGAA ACGAAGGCAA GCTCTCCTGG AACAGCAGCG CAAGGAGCAG GAGCGCCTGG CCCAGCTGGA GCGGGCGGAG CAGGAGAGGA AGGAGCGTGA GCGCCAGGAG CAAGAGCGCA AAAGACAACT GGAACTGGAG AAGCAACTGG AAAAGCAGCG GGAGCTAGAA CGGCAGAGAG AGGAGGAGAG GAGGAAAGAA ATTGAGAGGC GAGAGGCTGC AAAACGGGAA CTTGAAAGGC AACGACAACT TGAGTGGGAA CGGAATCGAA GGCAAGAACT ACTAAATCAA AGAAACAAAG AACAAGAGGA CATAGTTGTA CTGAAAGCAA AGAAAAAGAC TTTGGAATTT GAATTAGAAG CTCTAAATGA TAAAAAGCAT CAACTAGAAG GGAAACTTCA AGATATCAGA TGTCGATTGA CCACCCAAAG GCAAGAAATT GAGAGCACAA ACAAATCTAG AGAGTTGAGA ATTGCCGAAA TCACCCATCT ACAGCAACAA TTACAGGAAT CTCAGCAAAT GCTTGGAAGA CTTATTCCAG AAAAACAGAT ACTCAATGAC CAATTAAAAC AAGTTCAGCA GAACAGTTTG CACAGAGATT CACTTGTTAC ACTTAAAAGA GCCTTAGAAG CAAAAGAACT AGCTCGGCAG CACCTACGAG ACCAACTGGA TGAAGTGGAG AAAGAAACTA GATCAAAACT ACAGGAGATT GATATTTTCA ATAATCAGCT GAAGGAACTA AGAGAAATAC ACAATAAGCA ACAACTCCAG AAGCAAAAGT CCATGGAGGC TGAACGACTG AAACAGAAAG AACAAGAACG AAAGATCATA GAATTAGAAA AACAAAAAGA AGAAGCCCAA AGACGAGCTC AGGAAAGGGA CAAGCAGTGG CTGGAGCATG TGCAGCAGGA GGACGAGCAT CAGAGACCAA GAAAACTCCA CGAAGAGGAA AAACTGAAAA GGGAGGAGAG TGTCAAAAAG AAGGATGGCG AGGAAAAAGG CAAACAGGAA GCACAAGACA AGCTGGGTCG GCTTTTCCAT CAACACCAAG AACCAGCTAA GCCAGCTGTC CAGGCACCCT GGTCCACTGC AGAAAAAGGT CCACTTACCA TTTCTGCACA GGAAAATGTA AAAGTGGTGT ATTACCGGGC ACTGTACCCC TTTGAATCCA GAAGCCATGA TGAAATCACT ATCCAGCCAG GAGACATAGT CATGGTTAAA GGGGAATGGG TGGATGAAAG CCAAACTGGA GAACCCGGCT GGCTTGGAGG AGAATTAAAA GGAAAGACAG GGTGGTTCCC TGCAAACTAT GCAGAGAAAA TCCCAGAAAA TGAGGTTCCC GCTCCAGTGA AACCAGTGAC TGATTCAACA TCTGCCCCTG CCCCCAAACT GGCCTTGCGT GAGACCCCCG CCCCTTTGGC AGTAACCTCT TCAGAGCCCT CCACGACCCC TAATAACTGG GCCGACTTCA GCTCCACGTG GCCCACCAGC ACGAATGAGA AACCAGAAAC GGATAACTGG GATGCATGGG CAGCCCAGCC CTCTCTCACC GTTCCAAGTG CCGGCCAGTT AAGGCAGAGG TCCGCCTTTA CTCCAGCCAC GGCCACTGGC TCCTCCCCGT CTCCTGTGCT AGGCCAGGGT GAAAAGGTGG AGGGGCTACA AGCTCAAGCC CTATATCCTT GGAGAGCCAA AAAAGACAAC CACTTAAATT TTAACAAAAA TGATGTCATC ACCGTCCTGG AACAGCAAGA CATGTGGTGG TTTGGAGAAG TTCAAGGTCA GAAGGGTTGG TTCCCCAAGT CTTACGTGAA ACTCATTTCA GGGCCCATAA GGAAGTCTAC AAGCATGGAT TCTGGTTCTT CAGAGAGTCC TGCTAGTCTA AAGCGAGTAG CCTCTCCAGC AGCCAAGCCG GTCGTTTCGG GAGAAGAATT TATTGCCATG TACACTTACG AGAGTTCTGA GCAAGGAGAT TTAACCTTTC AGCAAGGGGA TGTGATTTTG GTTACCAAGA AAGATGGTGA CTGGTGGACA GGAACAGTGG GCGACAAGGC CGGAGTCTTC CCTTCTAACT ATGTGAGGCT TAAAGATTCA GAGGGCTCTG GAACTGCTGG GAAAACAGGG AGTTTAGGAA AAAAACCTGA AATTGCCCAG GTTATTGCCT CATACACCGC CACCGGCCCC GAGCAGCTCA CTCTCGCCCC TGGTCAGCTG ATTTTGATCC GAAAAAAGAA CCCAGGTGGA TGGTGGGAAG GAGAGCTGCA AGCACGTGGG AAAAAGCGCC AGATAGGCTG GTTCCCAGCT AATTATGTAA AGCTTCTAAG CCCTGGGACG AGCAAAATCA CTCCAACAGA GCCACCTAAG TCAACAGCAT TAGCGGCAGT GTGCCAGGTG ATTGGGATGT ACGACTACAC CGCGCAGAAT GACGATGAGC TGGCCTTCAA CAAGGGCCAG ATCATCAACG TCCTCAACAA GGAGGACCCT GACTGGTGGA AAGGAGAAGT CAATGGACAA GTGGGGCTCT TCCCATCCAA TTATGTGAAG CTGACCACAG ACATGGACCC AAGCCAGCAA TGGTGTTCAG ACTTACATCT CTTGGATATG TTGACCCCAA CTGAAAGAAA GCGACAAGGA TACATCCACG AGCTCATTGT CACCGAGGAG AACTATGTGA ATGACCTGCA GCTGGTCACA GAGATTTTTC AAAAACCCCT GATGGAGTCT GAGCTGCTGA CAGAAAAAGA GGTTGCTATG ATTTTTGTGA ACTGGAAGGA GCTGATTATG TGTAATATCA AACTACTAAA AGCGCTGAGA GTCCGCAAGA AGATGTCCGG GGAGAAGATG CCTGTGAAGA TGATTGGAGA CATCCTGAGC GCACAGCTGC CGCACATGCA GCCCTACATC CGCTTCTGCA GCCGCCAGCT CAACGGGGCT GCCCTGATCC AGCAGAAGAC GGACGAGGCC CCAGACTTCA AGGAGTTCGT CAAAAGATTG GCAATGGATC CTCGGTGTAG AGGGATGCCA CTCTCTAGTT TTATACTGAA GCCTATGCAA CGGGTAACAA GATACCCACT GATCATTAAA AATATCCTGG AAAACACCCC TGAAAACCAC CCGGACCACA GCCACTTGAA GCACGCCCTG GAGAAGGCGG AAGAGCTCTG TTCCCAGGTG AACGAAGGGG TGCGGGAGAA GGAGAACTCT GACCGGCTGG AGTGGATCCA GGCCCACGTG CAGTGTGAAG GCCTGTCTGA GCAACTTGTG TTCAATTCAG TGACCAATTG CTTGGGGCCG CGCAAATTTC TGCACAGTGG GAAGCTCTAC AAGGCCAAGA GCAACAAGGA GCTGTATGGC TTCCTTTTCA ACGACTTCCT CCTGCTGACT CAGATCACGA AGCCTTTGGG GTCTTCTGGC ACCGACAAAG TCTTCAGCCC CAAATCAAAC CTGCAGTATA AAATGTATAA AACACCTATT TTCCTAAATG AGGTTCTAGT AAAATTACCC ACCGACCCTT CTGGAGACGA GCCCATCTTC CACATCTCCC ACATTGACCG CGTCTATACT CTCCGAGCAG AAAGCATAAA TGAAAGGACT GCCTGGGTGC AGAAAATCAA AGCTGCTTCT GAACTCTACA TAGAGACTGA GAAAAAGAAG CGCGAGAAAG CGTACCTGGT CCGTTCCCAA AGGGCAACAG GCATTGGAAG GTTGATGGTG AACGTGGTTG AAGGCATCGA GTTGAAACCC TGTCGGTCAC ATGGAAAGAG CAACCCGTAC TGTGAGGTGA CCATGGGTTC CCAGTGCCAC ATCACCAAGA CGATCCAGGA CACTCTGAAC CCCAAGTGGA ATTCCAACTG CCAGTTCTTC ATCCGAGACC TGGAGCAGGA AGTCCTCTGC ATCACTGTGT TCGAGAGGGA CCAGTTCTCA CCAGATGATT TTTTGGGTCG GACGGAGATC CGTGTGGCGG ACATCAAGAA AGACCAGGGC TCCAAAGGTC CAGTTACGAA GTGTCTTCTG CTGCACGAAG TCCCCACGGG AGAGATTGTG GTCCGCTTGG ACCTGCAGTT GTTTGATGAG Protein function: Adapter protein that provides a link between the endocytic membrane traffic and the actin assembly machinery (PubMed:11584276, PubMed:29887380). Acts as guanine nucleotide exchange factor (GEF) for CDC42, and thereby stimulates actin nucleation mediated by WASL and the ARP2/3 complex (PubMed:11584276). Plays a role in the assembly and maturation of clathrin-coated vesicles. Recruits FCHSD2 to clathrin-coated pits (PubMed:29887380). Involved in endocytosis of activated EGFR, and probably also other growth factor receptors. Involved in endocytosis of integrin beta-1 (ITGB1) and transferrin receptor (TFR), internalization of ITGB1 as DAB2-dependent cargo but not TFR may involve association with DAB2 (PubMed:22648170). Promotes ubiquitination and subsequent degradation of EGFR, and thereby contributes to the down-regulation of EGFR-dependent signaling pathways. In chromaffin cells, required for normal exocytosis of catecholamines. Required for rapid replenishment of release-ready synaptic vesicles at presynaptic active zones. Inhibits ARHGAP31 activity toward RAC1 (PubMed:11744688). [The UniProt Consortium]