INSIG2 (Vector Vector will be determined during the manufacturing process, either pENTR223.1 or pUC,

Length: 678 Sequence: atggcagaag gagagacaga gtcacctggg cccaaaaagt gtggcccata tatttcatct gtcactagcc agagtgtgaa cttgatgatt cgaggagtag tgctattttt tattggagta tttcttgcat tagtgttaaa tttacttcag attcagagaa atgtgacgct ctttccacct gatgtgattg caagcatctt ttcttctgca tggtgggtac ccccatgctg tggcacggct tcagctgtga ttgggttatt atacccctgc attgacagac atctaggaga accacataaa tttaaaagag agtggtccag tgtaatgcgg tgtgtagcag tctttgttgg tataaatcat gccagtgcta aagtggattt cgataacaac atacagttgt ctctcacact ggctgcacta tccattggac tgtggtggac ttttgataga tctagaagtg gttttggcct tggagtagga attgccttct tggcaactgt ggtcactcaa ctgctagtat ataatggtgt ttaccaatat acatctccag atttcctcta tgttcgttct tggttaccat gtatattttt tgctggaggc ataacaatgg gaaacattgg tcgacaactg gcaatgtacg aatgtaaagt tatcgcagaa aaatctcatc Protein function: Oxysterol-binding protein that mediates feedback control of cholesterol synthesis by controlling both endoplasmic reticulum to Golgi transport of SCAP and degradation of HMGCR (PubMed:12242332, PubMed:16606821, PubMed:32322062). Acts as a negative regulator of cholesterol biosynthesis by mediating the retention of the SCAP-SREBP complex in the endoplasmic reticulum, thereby blocking the processing of sterol regulatory element-binding proteins (SREBPs) SREBF1/SREBP1 and SREBF2/SREBP2 (PubMed:32322062). Binds oxysterol, including 22- hydroxycholesterol, 24-hydroxycholesterol, 25-hydroxycholesterol and 27-hydroxycholesterol, regulating interaction with SCAP and retention of the SCAP-SREBP complex in the endoplasmic reticulum (PubMed:26160948, PubMed:17428920, PubMed:32322062). In presence of oxysterol, interacts with SCAP, retaining the SCAP-SREBP complex in the endoplasmic reticulum, thereby preventing SCAP from escorting SREBF1/SREBP1 and SREBF2/SREBP2 to the Golgi (PubMed:32322062). Sterol deprivation or phosphorylation by PCK1 reduce oxysterol-binding, disrupting the interaction between INSIG2 and SCAP, thereby promoting Golgi transport of the SCAP-SREBP complex, followed by processing and nuclear translocation of SREBF1/SREBP1 and SREBF2/SREBP2 (PubMed:32322062). Also regulates cholesterol synthesis by regulating degradation of HMGCR: initiates the sterol-mediated ubiquitin-mediated endoplasmic reticulum-associated degradation (ERAD) of HMGCR via recruitment of the reductase to the ubiquitin ligase RNF139 (PubMed:16606821, PubMed:22143767). [The UniProt Consortium]