H6PD (Vector pUC, Accession No. BC081559)

Length: 2376 Sequence: atgtggaata tgctcatagt ggcgatgtgc ttggcccttc tgggctgcct gcaagcccag gagctccagg gacatgtctc cataatcctg ctgggagcaa ctggggacct ggctaagaag tacttatggc agggactgtt ccagctgtac ctggatgaag cggggagggg tcacagtttt agcttccatg gagctgctct gacagccccc aagcagggtc aagagctcat ggccaaggcc ctggaatccc tctcctgccc caaggacatg gcacccagtc actgtgcaga gcacaaggat cagttcctgc agctgagcca gtaccgccaa ctgaagacgg ccgaggacta tcaggccctg aacaaggaca tcgaggcaca gctccagcac gcaggcctcc gggaggctgg caggatcttc tacttctcag tgccaccctt cgcctatgaa gacattgccc gcaacatcaa cagtagctgc cggccaggcc cgggcgcctg gctgcgggtt gtccttgaga aaccctttgg ccatgaccac ttctcagccc agcagctggc cacagaactc gggacctttt tccaggagga ggagatgtac cgggtggacc attacttagg caagcaggct gtggcacaga tcctgccttt ccgagaccag aaccgcaagg ctttggacgg cctctggaac cggcaccatg tggagcgggt ggagatcatc atgaaagaga ccgtggatgc cgaaggccgc accagcttct atgaggagta cggtgtcatt cgcgacgtcc tccagaacca tctgacggag gtcctcaccc tcgtggccat ggagctgccc cacaatgtca gcagtgcgga ggctgtgctg cggcacaagc ttcaggtctt ccaggcgctg cggggcctgc agaggggcag tgccgtcgtg ggccagtacc agtcttacag tgagcaggtg cgcagagagc tgcagaagcc agacagcttc cacagcctga cgccgacctt cgcagccgtc ctagtgcaca ttgacaacct tcgctgggag ggcgtgcctt tcatcctgat gtctggcaaa gccttggacg agagagtggg ctacgctcgg atcttgttca agaaccaggc ctgctgtgtg cagagcgaaa agcactgggc cgcggcgcag agccagtgcc tgccccggca gctcgtcttc cacatcggcc atggcgacct gggcagccct gccgtgctgg tcagcaggaa cctgttcagg ccctccctgc cctccagctg gaaggaaatg gagggaccac ctgggctccg ccttttcggc agccctctgt ccgattacta cgcctacagc cctgtgcagg agcgggacgc ccactccgtc ctcttatccc atatcttcca tggccggaag aatttcttca tcaccacaga gaacttgctg gcctcctgga acttctggac ccctctgctg gagagcctgg cccataaggc cccacgcctc taccctggag gagctgagaa tggccgtctg ttggactttg agttcagtag cggccggttg ttcttttccc agcagcagcc ggagcagctg gtgccagggc cagggccggc cccaatgccc agtgacttcc aggtcctcag ggccaagtac cgagagagcc cgctggtctc cgcctggtcc gaggagctga tctctaagct ggctaatgac atcgaggcca ccgctgtgcg agccgtgcgg cgctttggcc agttccacct ggcactgtcg gggggctcga gccccgtggc cctgttccag cagctggcca cggcgcacta tggcttcccc tgggcccaca cgcacctgtg gctggttgac gagcgctgcg tcccactctc agacccggag tccaacttcc agggcctgca ggcccacctg ctgcagcacg tccggatccc ctactacaac atccacccca tgcctgtgca cctgcagcag cggctctgcg ccgaggagga ccagggcgcc cagatctatg ccagggagat ctcagccctg gtggccaaca gcagcttcga cctggtgctg ctgggcatgg gtgccgacgg gcacacagcc tccctcttcc cacagtcacc cactggcctg gatggcgagc agctggtcgt gctgaccacg agcccctccc agccacaccg ccgcatgagc cttagcctgc ctctcatcaa ccgcgccaag aaggtggcag tcctggtcat gggcaggatg aagcgtgaga tcaccacgct ggtgagccgg gtgggccatg agcccaagaa gtggcccatc tcgggtgtcc tgccgcactc cggccagctg gtgtggtaca tggactacga cgccttcctg Protein function: Bifunctional enzyme localized in the lumen of the endoplasmic reticulum that catalyzes the first two steps of the oxidative branch of the pentose phosphate pathway/shunt, an alternative to glycolysis and a major source of reducing power and metabolic intermediates for biosynthetic processes. Has a hexose-6-phosphate dehydrogenase activity, with broad substrate specificity compared to glucose-6-phosphate 1-dehydrogenase/G6PD, and catalyzes the first step of the pentose phosphate pathway (PubMed:12858176, PubMed:18628520, PubMed:23132696). In addition, acts as a 6-phosphogluconolactonase and catalyzes the second step of the pentose phosphate pathway. May have a dehydrogenase activity for alternative substrates including glucosamine 6-phosphate and glucose 6-sulfate. The main function of this enzyme is to provide reducing equivalents such as NADPH to maintain the adequate levels of reductive cofactors in the oxidizing environment of the endoplasmic reticulum (PubMed:12858176, PubMed:18628520, PubMed:23132696). By producing NADPH that is needed by reductases of the lumen of the endoplasmic reticulum like corticosteroid 11-beta-dehydrogenase isozyme 1/HSD11B1, indirectly regulates their activity (PubMed:18628520). [The UniProt Consortium]