GPS2 (Vector pENTR223.1, Accession No. BC013652)

Length: 984 Sequence: atgcccgcac tcctggagcg ccccaagctt tccaacgcca tggccagggc gctgcaccgg cacattatga tggagcggga gcgcaagcgg caggaggaag aagaggtgga taagatgatg gaacagaaga tgaaggaaga acaggagaga aggaagaaaa aggagatgga agagagaatg tcattagagg agaccaagga acaaattctg aagttggagg agaagctttt ggctctacag gaagagaagc accagctttt cctgcagctc aagaaagttt tacatgagga agaaaaacgg aggcgaaagg aacagagtga cctgaccacc ctaacatcag ctgcatacca gcagagcctg actgttcaca caggaactca tctcctcagc atgcagggga gccctggagg acacaatcgc ccaggcaccc tcatggcagc tgacagagcc aaacaaatgt ttggacccca agtgcttacg acccggcact acgtgggctc agcagctgct tttgcaggga caccagagca tggacaattc caaggcagtc ctggtggtgc ctatgggact gctcagcccc cacctcacta tgggcccaca cagccagctt atagtcctag tcagcagctc agagctcctt cggcattccc tgcagtgcag tacctatctc agccacagcc acagccctat gctgtgcatg gccactttca gcccactcag acaggtttcc tccagcctgg tggtgccctg tccttgcaaa agcagatgga acatgctaac cagcagactg gcttctccga ctcatcctct ctgcgcccca tgcaccccca ggctctgcat ccagcccctg gactccttgc ttccccccag ctccctgtgc agatgcagcc agcaggaaag tcgggctttg cagctaccag ccaacctggc cctcggctcc ccttcatcca acacagccag aacccgcgat tctaccacaa Protein function: Key regulator of inflammation, lipid metabolism and mitochondrion homeostasis that acts by inhibiting the activity of the ubiquitin-conjugating enzyme UBE2N/Ubc13, thereby inhibiting 'Lys-63'- linked ubiquitination. In the nucleus, can both acts as a corepressor and coactivator of transcription, depending on the context (PubMed:24943844). Acts as a transcription coactivator in adipocytes by promoting the recruitment of PPARG to promoters: acts by inhibiting the activity of the ubiquitin-conjugating enzyme UBE2N/Ubc13, leading to stabilization of KDM4A and subsequent histone H3 'Lys-9' (H3K9) demethylation. Promotes cholesterol efflux by acting as a transcription coactivator (PubMed:19481530). Acts as a regulator of B-cell development by inhibiting UBE2N/Ubc13, thereby restricting the activation of Toll-like receptors (TLRs) and B-cell antigen receptors (BCRs) signaling pathways. Acts as a key mediator of mitochondrial stress response: in response to mitochondrial depolarization, relocates from the mitochondria to the nucleus following desumoylation and specifically promotes expression of nuclear-encoded mitochondrial genes (PubMed:29499132). Promotes transcription of nuclear-encoded mitochondrial genes by inhibiting UBE2N/Ubc13 (PubMed:29499132). Can also act as a corepressor as part of the N-Cor repressor complex by repressing active PPARG (PubMed:19858209, PubMed:24943844). Plays an anti-inflammatory role in macrophages and is required for insulin sensitivity by acting as a corepressor. Plays an anti-inflammatory role during the hepatic acute phase response by interacting with sumoylated NR1H2 and NR5A2 proteins, thereby preventing N-Cor corepressor complex dissociation (PubMed:20159957). In the cytosol, also plays a non- transcriptional role by regulating insulin signaling and pro- inflammatory pathways. In the cytoplasm, acts as a negative regulator of inflammation by inhibiting the proinflammatory TNF-alpha pathway, acts by repressing UBE2N/Ubc13 activity. In the cytoplasm of adipocytes, restricts the activation of insulin signaling via inhibition of UBE2N/Ubc13-mediated ubiquitination of AKT. Able to suppress G-protein- and mitogen-activated protein kinase-mediated signal transduction (PubMed:8943324). Acts as a tumor-suppressor in liposarcoma (PubMed:27460081). [The UniProt Consortium]