GLI3 (Vector pUC, Accession No. BC113616)

Length: 4743 Sequence: atggaggccc agtcccacag ctccacgacc actgaaaaga aaaaagttga gaattccata gtgaagtgct ccactcgaac agatgtgagc gagaaagccg ttgcctccag caccacttct aatgaggatg aaagtcctgg acagacttat cacagagaga gaagaaacgc aatcactatg cagccacaga atgtccaggg gctcagcaaa gtcagtgagg aaccttcaac atcgagtgac gagagggcct cattgatcaa gaaagagatc catgggtccc tgccacacgt ggcggagccc tctgtgccgt accgcgggac ggtgtttgcc atggacccca ggaatggtta catggagccc cactaccacc ctcctcatct tttccctgcc ttccatcctc ctgtaccaat tgatgccaga catcatgagg gccgttacca ttacgatcca tctccgattc ctccattgca tatgacttcc gccttatcta gtagccctac gtatccggac ctgcccttca ttaggatctc cccacaccgg aaccccgctg ctgcttccga gtctcccttc agccctccac atccctacat taatccctac atggactata tccgctcctt gcacagcagc ccatcgctct ccatgatctc agcaacccgt gggctgagcc ctacagatgc gccccatgca ggagtcagcc cagcagaata ctatcatcag atggccctgc taactggcca gcgcagcccc tatgcagaca ttattccctc agctgccacc gccggcacgg gggccatcca catggaatat cttcatgcta tggatagcac cagattctcc agccccaggc tgtcagccag gccgagccga aaacgtacac tgtccatatc accactctcc gatcatagct ttgaccttca gaccatgata aggacgtctc ccaactcctt ggtcacgatt ctcaataatt cccgtagcag ctcttcagca agtggctcct atggtcactt atctgcaagt gcaatcagcc ctgccttgag cttcacctac tcttccgcgc ccgtctctct ccacatgcat cagcagatcc taagccgaca acagagctta ggttcagcct ttggacacag ccctccactc atccaccctg ccccaacttt tccaacacag aggcctattc cagggatccc tacggttctg aaccccgtcc aggtcagctc cggcccttct gagtcctcac agaacaagcc cacgagtgag tctgcagtga gcagcactgg tgacccgatg cacaacaaga ggtccaagat caaacccgat gaagacctcc ccagcccagg ggctcggggg cagcaggaac agcccgaagg aacaaccctt gtcaaggagg aaggggacaa agatgaaagc aaacaggagc ctgaagtcat ctatgagaca aactgccact gggaaggctg cgcgagggag ttcgacaccc aagagcagct tgtgcaccat ataaataacg accatattca tggagagaag aaggagttcg tgtgcaggtg gctggactgc tcaagagagc agaaaccctt caaagcccag tatatgttgg tagtgcatat gagaagacac acgggcgaga agcctcacaa atgcactttt gaaggttgca caaaggccta ctcgagacta gaaaacttga aaacacactt gagatctcac actggagaga aaccatacgt ctgtgagcac gaaggttgca acaaggcttt ctcaaatgcc tctgatcgcg ccaaacacca aaacagaacg cattccaatg agaaaccata tgtgtgcaaa atcccaggct gcactaagcg ttacacagac ccaagctccc tccggaaaca tgtgaagaca gtgcatggcc cagaggctca tgtcaccaag aagcagcgag gggacatcca tcctcggccg ccacccccga gagattccgg cagccattca cagtccaggt cgcctggccg accgactcag ggagcccttg gtgagcagca ggacctcagc aacactacct caaagcggga agaatgcctc caggtgaaaa ccgtcaaggc agagaagcca atgacatctc agccaagccc tggtggtcag tcttcatgca gcagccaaca gtcccccatc agcaactatt ccaacagtgg gctcgagctt cctctgaccg atggaggtag tataggagac ctcagtgcca tcgatgaaac cccaatcatg gactcaacca tttccactgc aaccacagcc cttgctttgc aagccaggag aaacccggca gggaccaaat ggatggagca cgtaaaacta gaaaggctaa aacaagtgaa tggaatgttt ccgcgactga accccattct accccctaaa gcccctgcgg tctctcctct cataggaaat ggcacacagt ccaacaacac ctgcagcttg ggtgggccca tgacgcttct cccgggcaga agcgacctct ctggggtgga cgtcactatg ctgaacatgc tcaacagaag ggacagcagc gccagcacca tcagctcggc ctacctgagc agccgccgct cctcagggat ctcgccctgc ttctccagcc gccgctccag cgaggcgtca caggccgagg gccggccgca gaacgtgagc gtggccgact cctacgaccc catctccacc gacgcctcgc gccgctccag cgaagccagc cagagcgacg gcctgcccag cctgctcagc ctcacgcccg cccagcagta ccgcctcaag gccaagtacg cggctgccac aggagggccg ccgccgacgc ccctgcccaa catggagagg atgagcctga agacgcgcct ggcgctgctc ggggatgccc tcgagcctgg cgtggccctg cctccagttc atgccccgag gaggtgcagc gacgggggag cccacggcta cgggcggcgc cacctgcagc cgcacgatgc gctgggccac ggcgtgagga gggccagcga cccggtgcgg acaggctccg agggcctggc cctgcctcgt gtgccgcgct tcagcagcct cagcagctgc aaccccccgg cgatggccac gtccgcggag aagcgcagtc tcgtgcttca gaattacacg cggcccgagg gcggccagtc ccgaaacttc cactcgtccc cctgtcctcc cagcatcacc gagaacgtca ccctggagtc cctgaccatg gacgctgatg ccaacctgaa cgatgaggat ttcctgccgg acgacgtggt gcagtattta aattcccaga accaagcagg gtacgagcag cacttcccca gcgccctccc ggacgacagc aaagtgcccc acgggcccgg tgactttgac gcgcccgggc tgccagacag ccacgctggc cagcagttcc atgccctcga gcagccctgc cccgagggca gcaaaaccga cctgcccatt cagtggaacg aagtcagctc cggaagcgcc gacctgtcct cctccaagct caagtgtggg ccgcggcccg ctgtgccgca gactcgcgcc tttgggttct gcaacggcat ggtcgtccac ccgcagaacc ccttgaggag cgggcctgct gggggctatc agaccctcgg ggagaacagc aacccctacg gtggcccaga gcacttgatg ctccacaaca gccccggaag tggcaccagt ggaaacgcct tccatgaaca gccctgtaag gccccgcagt atgggaactg tctcaacagg cagccagtgg cccctggtgc actcgacggt gcctgtggtg ccgggattca agcctcaaag ctgaagagca cccccatgca agggagcggg ggccagctga atttcggcct gccggtagcg ccaaatgagt cagctggcag catggtgaat ggcatgcaga accaggaccc agtgggacag gggtacctgg ctcaccagct cctcggcgac agcatgcagc acccgggggc aggccgcccc ggtcagcaga tgcttgggca gattagtgct acctcacaca tcaacatcta ccaagggcca gagagctgcc tgccaggggc tcacggcatg ggcagccagc cgtcaagctt ggcagttgtc aggggctacc agccatgtgc cagctttggg ggcagcaggc gccaggctat gccgagggac agccttgctc tgcagtcagg acagctcagt gacacaagtc agacctgcag ggtgaatggt atcaagatgg agatgaaagg gcagccccat ccgctgtgct ctaatctgca gaattactct ggtcagttct atgaccaaac cgtgggcttc agtcagcaag acacgaaagc tggttcattc tctatttcag acgccagctg cctgctacag gggaccagcg ccaaaaactc tgagttactt tccccaggtg ctaatcaggt gacaagcaca gtggacagcc tcgacagcca tgacctggaa ggggtacaga ttgacttcga tgccatcata gacgatgggg accactccag cctgatgtcg ggggccctga gcccaagtat cattcagaac ctttcccata gctcctcccg cctcaccacg cctcgggcgt ccctcccatt cccagcgctg tccatgagca ccaccaacat ggctatcggg gacatgagtt ctttgctgac ctccctagcg gaagaaagca aattccttgc agttatgcaa Protein function: Has a dual function as a transcriptional activator and a repressor of the sonic hedgehog (Shh) pathway, and plays a role in limb development. The full-length GLI3 form (GLI3FL) after phosphorylation and nuclear translocation, acts as an activator (GLI3A) while GLI3R, its C-terminally truncated form, acts as a repressor. A proper balance between the GLI3 activator and the repressor GLI3R, rather than the repressor gradient itself or the activator/repressor ratio gradient, specifies limb digit number and identity. In concert with TRPS1, plays a role in regulating the size of the zone of distal chondrocytes, in restricting the zone of PTHLH expression in distal cells and in activating chondrocyte proliferation. Binds to the minimal GLI- consensus sequence 5'-GGGTGGTC-3'. [The UniProt Consortium]