GBF1 (Vector pUC, Accession No. BC117681)

Length: 5568 Sequence: ATGGTGGATA AGAATATTTA CATCATTCAA GGGGAGATTA ACATTGTGGT TGGGGCCATC AAACGAAATG CCCGATGGAG CACCCATACA CCACTGGATG AAGAACGGGA TCCTCTGCTG CATAGTTTCG GTCATCTAAA GGAGGTTTTA AACAGTATAA CAGAACTCTC AGAAATTGAG CCCAATGTAT TCCTTCGACC TTTTCTGGAA GTGATTCGCT CTGAAGATAC CACTGGCCCT ATCACTGGAC TGGCACTCAC CTCTGTCAAC AAGTTCCTGT CCTATGCACT CATAGATCCC ACCCATGAGG GCACAGCAGA GGGCATGGAG AACATGGCAG ATGCTGTCAC CCATGCTCGT TTTGTGGGCA CGGATCCTGC CAGTGATGAA GTTGTCCTGA TGAAAATCCT TCAGGTTCTA CGGACTCTGC TGCTAACCCC AGTGGGTGCC CACCTAACCA ATGAATCTGT GTGTGAGATT ATGCAGTCTT GCTTCCGGAT CTGCTTTGAA ATGAGGCTCA GTGAGTTATT GAGAAAATCC GCAGAGCACA CTCTCGTAGA CATGGTGCAG CTGCTCTTCA CAAGGTTACC TCAGTTTAAA GAAGAACCCA AGAACTATGT GGGGACCAAC ATGAAGAAGC TGAAAATGAG AGCCGGAGGC ATGAGTGATT CATCCAAATG GAAGAAACAG AAGAGATCCC CTCGGCCCCC ACGCCATATG ACCAAAGTCA CACCAGGTTC AGAGCTGCCC ACTCCCAATG GAACCACCTT ATCATCTAAC CTCACTGGTG GCATGCCCTT CATTGATGTG CCCACTCCCA TCTCCTCTGC AAGTTCAGAA GCTGCCTCAG CAGTGGTCAG TCCCTCTACA GACAGTGGCC TGGAATTCTC CTCCCAAACC ACTTCCAAGG AAGACCTTAC TGATCTAGAG CAACCTGGCT CTCCAGGGTA CAGCACAGCT ACAGAGCCTG GAAGCAGTGA GCTAGGTGTT CCCGAGCAGC CTGACCTCCA GGAAGGGACC CATGTGGAAA AGTCCCAGTC AGCATCTGTG GAGTCCATCC CTGAAGTGTT AGAGGAGTGC ACGTCCCCTG CCGACCACTC TGACTCTGCC TCTGTCCATG ACATGGATTA CGTCAATCCC CGGGGCGTGC GCTTTACACA GTCCTCCCAG AAAGAAGGCA CAGCTTTGGT CCCCTATGGT CTTCCCTGCA TCCGCGAGCT CTTCCGCTTC CTCATCTCCC TCACCAATCC ACACGACCGC CATAACTCAG AGGTTATGAT TCACATGGGA CTGCATTTGC TGACAGTGGC CCTTGAGTCA GCCCCTGTAG CCCAGTGCCA GACCCTCTTG GGCCTCATCA AGGATGAGAT GTGCCGTCAC TTATTCCAGC TACTCAGCAT AGAGCGACTA AACCTTTATG CTGCTTCCCT GCGAGTATGC TTCCTACTGT TTGAGAGCAT GCGAGAGCAC CTCAAGTTCC AAATGGAGAT GTACATCAAA AAGCTTATGG AGATCATCAC TGTGGAGAAC CCCAAGATGC CTTATGAGAT GAAGGAGATG GCACTGGAGG CCATTGTGCA GCTCTGGTGC ATCCCCAGCT TTGTCACAGA GCTCTACATC AACTATGATT GTGACTACTA CTGTTCCAAC CTCTTTGAGG AACTCACAAA GCTGCTGTCC AAGAATGCCT TCCCTGTGTC TGGTCAACTC TATACAACAC ACCTACTATC TCTTGATGCC CTATTGACAG TGATTGACAG CACCGAGGCC CACTGCCAGG CTAAAGTCCT CAACAGCCTC ACCCAGCAAG AGAAGAAGGA GACAGCCAGA CCAAGCTGTG AGATAGTAGA TGGCACCCGA GAAGCTAGCA ATACTGAGAG AACTGCCAGC GATGGGAAAG CTGTAGGCAT GGCCTCAGAC ATCCCAGGCC TGCATCTGCC AGGTGGAGGG CGGCTGCCAC CAGAACATGG GAAATCAGGA TGCAGTGATC TGGAGGAAGC TGTTGACTCT GGGGCTGACA AAAAGTTTGC CCGGAAGCCA CCCCGATTTT CCTGTCTCCT GCCAGATCCA CGGGAACTAA TTGAAATTAA AAACAAAAAG AAGCTGCTAA TCACTGGCAC AGAGCAGTTC AATCAGAAAC CAAAGAAGGG GATTCAGTTT CTGCAAGAGA AAGGCCTCCT CACCATCCCA ATGGACAACA CAGAGGTTGC TCAGTGGCTC CGAGAGAACC CTCGGCTGGA CAAGAAGATG ATTGGAGAGT TTGTGAGTGA CCGCAAAAAC ATTGACCTGT TGGAGAGCTT TGTGAGCACC TTCAGTTTTC AGGGTCTGCG ACTGGACGAA GCCCTCCGCC TCTACCTGGA AGCCTTCCGT TTGCCTGGGG AAGCACCAGT CATCCAGAGG TTGCTGGAGG CATTCACAGA GCGTTGGATG AATTGTAATG GCTCCCCATT TGCCAATAGC GATGCCTGCT TTTCCCTGGC CTATGCTGTC ATCATGCTTA ATACTGACCA GCACAACCAC AATGTTCGTA AACAGAATGC ACCCATGACC CTGGAGGAGT TTCGCAAAAA TCTGAAAGGT GTGAATGGAG GCAAGGACTT TGAGCAAGAC ATCCTGGAGG ACATGTACCA TGCCATCAAG AATGAGGAAA TTGTAATGCC TGAGGAGCAG ACAGGCTTGG TTCGGGAGAA CTATGTGTGG AATGTGCTGC TTCATCGAGG TGCCACCCCT GAGGGCATAT TCCTGCGTGT GCCTACTGCC AGCTATGATC TTGACCTCTT CACCATGACC TGGGGCCCCA CTATTGCTGC TCTCTCTTAT GTCTTTGACA AAAGCCTTGA GGAGACAATC ATCCAGAAAG CCATCTCAGG CTTCAGGAAG TGCGCCATGA TCTCCGCCCA CTATGGCCTC AGCGATGTGT TTGACAATCT CATCATCTCT CTATGCAAAT TCACAGCTCT CAGCAGTGAG TCTATTGAGA ACCTGCCCAG TGTATTTGGA AGCAACCCTA AAGCCCATAT TGCAGCCAAG ACAGTATTCC ATTTGGCCCA TCGTCATGGT GACATCCTGC GGGAGGGCTG GAAGAATATC ATGGAGGCCA TGCTGCAGCT CTTCCGAGCC CAACTACTGC CCAAGGCTAT GATAGAGGTA GAAGATTTCG TGGATCCCAA TGGCAAGATC TCTCTACAGC GGGAAGAGAC ACCATCAAAC CGAGGAGAGT CAACAGTGCT GAGCTTTGTG AGCTGGCTAA CACTGAGTGG TCCTGAGCAG TCTAGTGTTC GGGGCCCATC CACTGAAAAC CAAGAGGCCA AGAGAGTGGC CTTAGAGTGT ATAAAGCAAT GTGACCCAGA AAAAATGATC ACAGAAAGCA AGTTCCTCCA GCTGGAGTCA CTACAGGAGC TCATGAAGGC TCTGGTCTCA GTGACACCAG ATGAAGAGAC ATATGATGAG GAAGATGCTG CTTTCTGCCT AGAGATGCTG CTAAGGATTG TGTTGGAGAA CAGGGATCGT GTGGGCTGTG TGTGGCAGAC TGTTCGAGAC CATCTATACC ACCTCTGTGT TCAGGCACAA GATTTCTGCT TCCTTGTGGA GCGGGCAGTG GTGGGGTTGC TACGCCTGGC CATTCGGCTT CTCCGGAGAG AAGAGATCAG TGCTCAGGTG CTGCTCTCCC TGCGCATTTT GCTACTGATG AAGCCCAGTG TGCTATCCCG AGTCAGCCAC CAGGTTGCGT ATGGGCTCCA TGAACTCCTG AAGACCAATG CAGCCAACAT CCACTCAGGT GATGACTGGG CCACACTCTT CACACTGCTG GAGTGCATCG GCTCAGGTGT GAAGCCTCCA GCTGCTCTGC AGGCCACAGC CAGGGCAGAT GCACCTGATG CCGGGGCCCA GTCAGATAGT GAGCTCCCAT CCTACCATCA GAATGACGTG AGCCTGGATC GAGGGTACAC TTCCGACTCA GAGGTCTACA CTGACCATGG CAGGCCGGGC AAGATACACC GATCAGCCAC AGATGCCGAT GTGGTCAACA GTGGTTGGTT AGTGGTTGGG AAGGATGACG TTGATAACTC CAAGCCAGGG CCCAGCCGCC CAGGCCCTTC ACCCCTGATC AATCAATACA GCCTAACAGT GGGACTGGAT TTGGGGCCAC ACGACACTAA GTCTCTGCTT AAGTGTGTGG AATCGCTGTC CTTCATCGTG CGTGATGCTG CCCACATCAC ACCTGACAAC TTTGAGCTCT GCGTCAAGAC TCTCCGGATC TTTGTGGAGG CCAGTCTGAA TGGCGGATGC AAGTCCCAGG AGAAACGTGG CAAGAGTCAC AAATATGACA GCAAAGGGAA CCGCTTCAAG AAGAAATCCA AAGAGGGATC AATGCTTCGC CGGCCTCGAA CCTCCAGCCA ACATGCCTCT CGGGGCGGGC AGAGTGATGA TGATGAGGAC GAAGGCGTGC CTGCCAGCTA CCATACGGTG TCTTTACAGT TGCTAGACCT GATGCACACC CTGCACACGC GGGCAGCCTC TATCTACAGC TCATGGGCGG AGGAGCAACG CCACCTGGAG ACAGGTGGCC AGAAGATTGA AGCTGATTCT CGCACCCTCT GGGCCCACTG CTGGTGCCCT TTACTGCAGG GTATTGCCTG CCTGTGCTGC GATGCCCGGC GCCAGGTACG GATGCAGGCA CTGACCTATC TGCAGCGAGC ACTACTTGTA CATGATCTGC AAAAGCTAGA TGCCCTGGAA TGGGAGTCCT GTTTTAACAA GGTGCTGTTT CCTCTACTTA CCAAGCTCTT GGAGAACATC AGCCCTGCAG ATGTGGGTGG GATGGAGGAG ACCCGGATGA GGGCTTCCAC ATTGCTCTCT AAGGTCTTCC TGCAGCACCT GTCTCCACTG CTGTCACTCT CTACCTTTGC GGCCCTCTGG CTCACCATCT TGGACTTCAT GGACAAGTAC ATGCACGCAG GCTCCAGCGA CTTACTGTCA GAGGCGATCC CTGAGTCTCT GAAGAACATG CTTCTGGTGA TGGACACAGC GGAGATTTTC CACAGTGCAG ATGCACGGGG AGGCGGCCCC TCGGCCCTCT GGGAGATCAC CTGGGAACGC ATTGACTGTT TTCTCCCTCA CCTACGAGAT GAACTCTTCA AGCAGACCGT CATCCAGGAC CCCATGCCCA TGGAGCCTCA AGGCCAAAAG CCTCTCGCCT CAGCCCACCT GACTTCCGCT GCTGGCGACA CTAGGACACC TGGCCATCCA CCGCCCCCAG AGATTCCATC TGAGCTGGGG GCCTGTGACT TTGAGAAGCC CGAGAGCCCC CGAGCCGCCA GCAGCAGCTC CCCAGGATCA CCAGTGGCCT CAAGCCCCAG CAGGCTGAGC CCCACCCCCG ACGGGCCTCC ACCCTTGGCT CAGCCCCCAC TGATCCTGCA GCCCTTGGCC TCCCCACTGC AGGTGGGCGT GCCACCTATG ACTCTGCCCA TCATCCTCAA CCCTGCGCTC ATCGAGGCCA CCTCACCAGT GCCCCTCCTG GCCACACCCC GCCCCACAGA TCCCATACCC ACCTCTGAGG Protein function: Guanine-nucleotide exchange factor (GEF) for members of the Arf family of small GTPases involved in trafficking in the early secretory pathway, its GEF activity initiates the coating of nascent vesicles via the localized generation of activated ARFs through replacement of GDP with GTP. Recruitment to cis-Golgi membranes requires membrane association of Arf-GDP and can be regulated by ARF1, ARF3, ARF4 and ARF5. Involved in the recruitment of the COPI coat complex to the endoplasmic reticulum exit sites (ERES), and the endoplasmic reticulum-Golgi intermediate (ERGIC) and cis-Golgi compartments which implicates ARF1 activation. Involved in COPI vesicle-dependent retrograde transport from the ERGIC and cis-Golgi compartments to the endoplasmic reticulum (ER) (PubMed:16926190, PubMed:17956946, PubMed:18003980, PubMed:12047556, PubMed:12808027, PubMed:19039328, PubMed:24213530). Involved in the trans-Golgi network recruitment of GGA1, GGA2, GGA3, BIG1, BIG2, and the AP-1 adapter protein complex related to chlathrin-dependent transport, the function requires its GEF activity (probably at least in part on ARF4 and ARF5) (PubMed:23386609). Has GEF activity towards ARF1 (PubMed:15616190). Has in vitro GEF activity towards ARF5. Involved in the processing of PSAP (PubMed:17666033). Required for the assembly of the Golgi apparatus (PubMed:12808027, PubMed:18003980). The AMPK- phosphorylated form is involved in Golgi disassembly during mitotis and under stress conditions (PubMed:18063581, PubMed:23418352). May be involved in the COPI vesicle-dependent recruitment of PNPLA2 to lipid droplets, however, this function is under debate (PubMed:19461073, PubMed:22185782). In neutrophils, involved in G protein-coupled receptor (GPCR)-mediated chemotaxis und superoxide production. Proposed to be recruited by phosphatidylinositol-phosphates generated upon GPCR stimulation to the leading edge where it recruits and activates ARF1, and is involved in recruitment of GIT2 and the NADPH oxidase complex (PubMed:22573891). Plays a role in maintaining mitochondrial morphology (PubMed:25190516). [The UniProt Consortium]