FZR1 (Vector Vector will be determined during the manufacturing process, either pENTR223.1 or pUC, A

Length: 1482 Sequence: atggaccagg actatgagcg gcgcctgctt cgccagatcg tcatccagaa tgagaacacg atgccacgcg tcacagagat gcggcggacc ctgacgcctg ccagctcccc agtgtcctcg cccagcaagc acggagaccg cttcatcccc tccagagccg gagccaactg gagcgtgaac ttccacagga ttaacgagaa tgagaagtct cccagtcaga accggaaagc caaggacgcc acctcagaca acggcaaaga cggcctggcc tactctgccc tgctcaagaa tgagctgctg ggtgccggca tcgagaaggt gcaggacccg cagactgagg accgcaggct gcagccctcc acgcctgaga agaagggtct gttcacgtat tcccttagca ccaagcgctc cagccccgat gacggcaacg atgtgtctcc ctactccctg tctcccgtca gcaacaagag ccagaagctg ctccggtccc cccggaaacc cacccgcaag atctccaaga tccccttcaa ggtgctggac gcgcccgagc tgcaggacga cttctacctc aatctggtgg actggtcgtc cctcaatgtg ctcagcgtgg ggctaggcac ctgcgtgtac ctgtggagtg cctgtaccag ccaggtgacg cggctctgtg acctctcagt ggaaggggac tcagtgacct ccgtgggctg gtctgagcgg gggaacctgg tggcggtggg cacacacaag ggcttcgtgc agatctggga cgcagccgca gggaagaagc tgtccatgtt ggagggccac acggcacgcg tcggggcgct ggcctggaat gctgagcagc tgtcgtccgg gagccgcgac cgcatgatcc tgcagaggga catccgcacc ccgccactgc agtcggagcg gcggctgcag ggccaccggc aggaggtgtg cgggctcaag tggtccacag accaccagct cctcgcctcg gggggcaacg acaacaagct gctggtctgg aatcactcga gcctgagccc cgtgcagcag tacacggagc acctggcggc cgtgaaggcc atcgcctggt ccccacatca gcacgggctg ctggcctcgg ggggcggcac agctgaccgc tgtatccgct tctggaacac gctgacagga caaccactgc agtgtatcga cacgggctcc caagtgtgca atctggcctg gtccaagcac gccaacgagc tggtgagcac gcacggctac tcacagaacc agatccttgt ctggaagtac ccctccctga cccaggtggc caagctgacc gggcactcct accgcgtgct gtacctggca atgtcccctg atggggaggc catcgtcact ggtgctggag acgagaccct gaggttctgg aacgtcttta gcaaaacccg ttcgacaaag gagtctgtgt ctgtgctcaa cctcttcacc aggatccggt Protein function: Substrate-specific adapter for the anaphase promoting complex/cyclosome (APC/C) E3 ubiquitin-protein ligase complex. Associates with the APC/C in late mitosis, in replacement of CDC20, and activates the APC/C during anaphase and telophase. The APC/C remains active in degrading substrates to ensure that positive regulators of the cell cycle do not accumulate prematurely. At the G1/S transition FZR1 is phosphorylated, leading to its dissociation from the APC/C. Following DNA damage, it is required for the G2 DNA damage checkpoint: its dephosphorylation and reassociation with the APC/C leads to the ubiquitination of PLK1, preventing entry into mitosis. Acts as an adapter for APC/C to target the DNA-end resection factor RBBP8/CtIP for ubiquitination and subsequent proteasomal degradation. Through the regulation of RBBP8/CtIP protein turnover, may play a role in DNA damage response, favoring DNA double-strand repair through error-prone non-homologous end joining (NHEJ) over error-free, RBBP8-mediated homologous recombination (HR) (PubMed:25349192). [The UniProt Consortium]