DCLRE1C (Vector Vector will be determined during the manufacturing process, either pENTR223.1 or pUC

Length: 1734 Sequence: atgaagcatc aggagaggtt tttatttcag ggcaataatg gaactgtcct gtacacagga gacttcagat tggcgcaagg agaagctgct agaatggagc ttctgcactc cgggggcaga gtcaaagaca tccaaagtgt atatttggat actacgttct gtgatccaag attttaccaa attccaagtc gggaggagtg tttaagtgga gtcttagagc tggtccgaag ctggatcact cggagcccgt accatgttgt gtggctgaac tgcaaagcgg cttatggcta tgaatatctg ttcaccaacc ttagtgaaga attaggagtc caggttcatg tgaataagct agacatgttt aggaacatgc ctgagatcct tcatcatctc acaacagacc gcaacactca gatccatgca tgccggcatc ccaaggcaga ggaatatttt cagtggagca aattaccctg tggaattact tccagaaata gaattccact ccacataatc agcattaagc catccaccat gtggtttgga gaaaggagca gaaaaacaaa tgtaattgtg aggactggag agagttcata cagagcttgt ttttcttttc actcctccta cagtgagatt aaagatttct tgagctacct ctgtcctgtg aacgcatatc caaatgtcat tccagttggc acaactatgg ataaagttgt cgaaatctta aagcctttat gccggtcttc ccaaagtacg gagccaaagt ataaaccact gggaaaactg aagagagcta gaacagttca ccgagactca gaggaggaag atgactatct ctttgatgat cctctgccaa tacctttaag gcacaaagtt ccatacccgg aaacttttca ccctgaggta ttttcaatga ctgcagtatc agaaaagcag cctgaaaaac tgagacaaac cccaggatgc tgcagagcag agtgtatgca gagctctcgt ttcacaaact ttgtagattg tgaagaatcc aacagtgaaa gtgaagaaga agtaggaatc ccagcttcac tgcaaggaga tctgggctct gtacttcacc tgcaaaaggc tgatggggat gtaccccagt gggaagtatt ctttaaaaga aatgatgaaa tcacagatga gagtttggaa aacttccctt cctccacagt ggcaggggga tctcagtcac caaagctttt cagtgactct gatggagaat caactcacat ctcctcccag aattcttccc agtcaacaca cataacagaa caaggaagtc aaggctggga cagccaatct gatactgttt tgttatcttc ccaagagaga aacagtgggg atattacttc cttggacaaa gctgactaca gaccaacaat caaagagaat attcctgcct ctctcatgga acaaaatgta atttgcccaa aggatactta ctctgatttg aaaagcagag ataaagatgt gacaatagtt cctagtactg gagaaccaac tactctaagc agtgagacac atatacccga ggaaaaaagt ttgctaaatc ttagcacaaa tgcagattcc cagagctctt ctgattttga agttccctca actccagaag ctgagttacc taaacgagag catttacaat atttatatga gaagctggca actggtgaga gtatagcagt caaaaaaaga aaatgctcac tcttagatac Protein function: Required for V(D)J recombination, the process by which exons encoding the antigen-binding domains of immunoglobulins and T-cell receptor proteins are assembled from individual V, (D), and J gene segments. V(D)J recombination is initiated by the lymphoid specific RAG endonuclease complex, which generates site specific DNA double strand breaks (DSBs). These DSBs present two types of DNA end structures: hairpin sealed coding ends and phosphorylated blunt signal ends. These ends are independently repaired by the non homologous end joining (NHEJ) pathway to form coding and signal joints respectively. This protein exhibits single-strand specific 5'-3' exonuclease activity in isolation and acquires endonucleolytic activity on 5' and 3' hairpins and overhangs when in a complex with PRKDC. The latter activity is required specifically for the resolution of closed hairpins prior to the formation of the coding joint. May also be required for the repair of complex DSBs induced by ionizing radiation, which require substantial end-processing prior to religation by NHEJ. [The UniProt Consortium]