DCLRE1C (Vector Vector will be determined during the manufacturing process, either pENTR223.1 or pUC

Length: 1305 Sequence: atgagttctt tcgaggggca gatggccgag tatccaacta tctccataga ccgcttcgat agggagaacc tgagggcccg cgcctacttc ctgtcccact gccacaaaga tcacatgaaa ggattaagag cccctacctt gaaaagaagg ttggagtgca gcttgaaggt ttatctatac tgttcacctg tgactaagga gttgttgtta acgagcccga aatacagatt ttggaagaaa cgaattatat ctattgaaat cgagactcct acccagatat ctttagtgga tgaagcatca ggagagaagg aagagattgt tgtgactctc ttaccagctg gtcactgtcc gggatcagtt atgtttttat ttcagggcaa taatggaact gtcctgtaca caggagactt cagattggcg caaggagaag ctgctagaat ggagcttctg cactccgggg gcagagtcaa agacatccaa agtgtatatt tggatactac gttctgtgat ccaagatttt accaaattcc aagtcgggag gagtgtttaa gtggagtctt agagctggtc cgaagctgga tcactcggag cccgtaccat gttgtgtggc tgaactgcaa agcggcttat ggctatgaat atttgttcac caaccttagt gaagaattag gagtccaggt tcatgtgaat aagctagaca tgtttaggaa catgcctgag atccttcgtc atctcacaac agaccgcaac actcagatcc atgcatgccg gcatcccaag gcagaggaat attttcagtg gagcaaatta ccctgtggaa ttacttccag aaatagaatt ccactccaca taatcagcat taagccatcc accatgtggt ttggagaaag gagcagaaaa acaaatgtaa ttgtgaggac tggagagagt tcatacagag cttgtttttc ttttcactcc tcctacagtg agattaaaga tttcttgagc tacctctgtc ctgtgaacgc atatccaaat gtcattccag ttggcacaac tatggataaa gttgtcgaaa tcttaaagcc tttatgccgg tcttcccaaa gtacggagcc aaagtataaa ccactgggaa aactgaagag agctagaaca gttcaccgag actcagggtc tcactctgtc actcaggcta gaatgcggtg gtgccatcat gactccctgt atcctttaac tcctgggatc aagcgatctt cctgcctcag cctcctgact agctggatca caggtgcata ccgccatgcc cagctaatga Protein function: Required for V(D)J recombination, the process by which exons encoding the antigen-binding domains of immunoglobulins and T-cell receptor proteins are assembled from individual V, (D), and J gene segments. V(D)J recombination is initiated by the lymphoid specific RAG endonuclease complex, which generates site specific DNA double strand breaks (DSBs). These DSBs present two types of DNA end structures: hairpin sealed coding ends and phosphorylated blunt signal ends. These ends are independently repaired by the non homologous end joining (NHEJ) pathway to form coding and signal joints respectively. This protein exhibits single-strand specific 5'-3' exonuclease activity in isolation and acquires endonucleolytic activity on 5' and 3' hairpins and overhangs when in a complex with PRKDC. The latter activity is required specifically for the resolution of closed hairpins prior to the formation of the coding joint. May also be required for the repair of complex DSBs induced by ionizing radiation, which require substantial end-processing prior to religation by NHEJ. [The UniProt Consortium]